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Expression of Androgen and Estrogen Receptors in the Human Lacrimal Gland

Lacrimal gland dysfunction causes dry eye disease (DED) due to decreased tear production. Aqueous-deficient DED is more prevalent in women, suggesting that sexual dimorphism of the human lacrimal gland could be a potential cause. Sex steroid hormones are a key factor in the development of sexual dim...

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Autores principales: Hat, Koraljka, Planinić, Ana, Ježek, Davor, Kaštelan, Snježana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10053362/
https://www.ncbi.nlm.nih.gov/pubmed/36982683
http://dx.doi.org/10.3390/ijms24065609
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author Hat, Koraljka
Planinić, Ana
Ježek, Davor
Kaštelan, Snježana
author_facet Hat, Koraljka
Planinić, Ana
Ježek, Davor
Kaštelan, Snježana
author_sort Hat, Koraljka
collection PubMed
description Lacrimal gland dysfunction causes dry eye disease (DED) due to decreased tear production. Aqueous-deficient DED is more prevalent in women, suggesting that sexual dimorphism of the human lacrimal gland could be a potential cause. Sex steroid hormones are a key factor in the development of sexual dimorphism. This study aimed to quantify estrogen receptor (ER) and androgen receptor (AR) expression in the human lacrimal gland and compare it between sexes. RNA was isolated from 35 human lacrimal gland tissue samples collected from 19 cornea donors. AR, ERα, and ERβ mRNA was identified in all samples, and their expression was quantified using qPCR. Immunohistochemical staining was performed on selected samples to evaluate protein expression of the receptors. ERα mRNA expression was significantly higher than the expression of AR and ERβ. No difference in sex steroid hormone (SSH) receptor mRNA expression was observed between sexes, and no correlation was observed with age. If ERα protein expression is found to be concordant with mRNA expression, it should be investigated further as a potential target for hormone therapy of DED. Further research is needed to elucidate the role of sex steroid hormone receptors in sex-related differences of lacrimal gland structure and disease.
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spelling pubmed-100533622023-03-30 Expression of Androgen and Estrogen Receptors in the Human Lacrimal Gland Hat, Koraljka Planinić, Ana Ježek, Davor Kaštelan, Snježana Int J Mol Sci Article Lacrimal gland dysfunction causes dry eye disease (DED) due to decreased tear production. Aqueous-deficient DED is more prevalent in women, suggesting that sexual dimorphism of the human lacrimal gland could be a potential cause. Sex steroid hormones are a key factor in the development of sexual dimorphism. This study aimed to quantify estrogen receptor (ER) and androgen receptor (AR) expression in the human lacrimal gland and compare it between sexes. RNA was isolated from 35 human lacrimal gland tissue samples collected from 19 cornea donors. AR, ERα, and ERβ mRNA was identified in all samples, and their expression was quantified using qPCR. Immunohistochemical staining was performed on selected samples to evaluate protein expression of the receptors. ERα mRNA expression was significantly higher than the expression of AR and ERβ. No difference in sex steroid hormone (SSH) receptor mRNA expression was observed between sexes, and no correlation was observed with age. If ERα protein expression is found to be concordant with mRNA expression, it should be investigated further as a potential target for hormone therapy of DED. Further research is needed to elucidate the role of sex steroid hormone receptors in sex-related differences of lacrimal gland structure and disease. MDPI 2023-03-15 /pmc/articles/PMC10053362/ /pubmed/36982683 http://dx.doi.org/10.3390/ijms24065609 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hat, Koraljka
Planinić, Ana
Ježek, Davor
Kaštelan, Snježana
Expression of Androgen and Estrogen Receptors in the Human Lacrimal Gland
title Expression of Androgen and Estrogen Receptors in the Human Lacrimal Gland
title_full Expression of Androgen and Estrogen Receptors in the Human Lacrimal Gland
title_fullStr Expression of Androgen and Estrogen Receptors in the Human Lacrimal Gland
title_full_unstemmed Expression of Androgen and Estrogen Receptors in the Human Lacrimal Gland
title_short Expression of Androgen and Estrogen Receptors in the Human Lacrimal Gland
title_sort expression of androgen and estrogen receptors in the human lacrimal gland
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10053362/
https://www.ncbi.nlm.nih.gov/pubmed/36982683
http://dx.doi.org/10.3390/ijms24065609
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