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Amantadine Toxicity in Apostichopus japonicus Revealed by Proteomics

Amantadine exposure can alter biological processes in sea cucumbers, which are an economically important seafood in China. In this study, amantadine toxicity in Apostichopus japonicus was analyzed by oxidative stress and histopathological methods. Quantitative tandem mass tag labeling was used to ex...

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Autores principales: Zhao, Junqiang, Chen, Jianqiang, Tian, Xiuhui, Jiang, Lisheng, Cui, Qingkui, Sun, Yanqing, Wu, Ningning, Liu, Ge, Ding, Yuzhu, Wang, Jing, Liu, Yongchun, Han, Dianfeng, Xu, Yingjiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10053536/
https://www.ncbi.nlm.nih.gov/pubmed/36976991
http://dx.doi.org/10.3390/toxics11030226
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author Zhao, Junqiang
Chen, Jianqiang
Tian, Xiuhui
Jiang, Lisheng
Cui, Qingkui
Sun, Yanqing
Wu, Ningning
Liu, Ge
Ding, Yuzhu
Wang, Jing
Liu, Yongchun
Han, Dianfeng
Xu, Yingjiang
author_facet Zhao, Junqiang
Chen, Jianqiang
Tian, Xiuhui
Jiang, Lisheng
Cui, Qingkui
Sun, Yanqing
Wu, Ningning
Liu, Ge
Ding, Yuzhu
Wang, Jing
Liu, Yongchun
Han, Dianfeng
Xu, Yingjiang
author_sort Zhao, Junqiang
collection PubMed
description Amantadine exposure can alter biological processes in sea cucumbers, which are an economically important seafood in China. In this study, amantadine toxicity in Apostichopus japonicus was analyzed by oxidative stress and histopathological methods. Quantitative tandem mass tag labeling was used to examine changes in protein contents and metabolic pathways in A. japonicus intestinal tissues after exposure to 100 µg/L amantadine for 96 h. Catalase activity significantly increased from days 1 to 3 of exposure, but it decreased on day 4. Superoxide dismutase and glutathione activities were inhibited throughout the exposure period. Malondialdehyde contents increased on days 1 and 4 but decreased on days 2 and 3. Proteomics analysis revealed 111 differentially expressed proteins in the intestines of A. japonicus after amantadine exposure compared with the control group. An analysis of the involved metabolic pathways showed that the glycolytic and glycogenic pathways may have increased energy production and conversion in A. japonicus after amantadine exposure. The NF-κB, TNF, and IL-17 pathways were likely induced by amantadine exposure, thereby activating NF-κB and triggering intestinal inflammation and apoptosis. Amino acid metabolism analysis showed that the leucine and isoleucine degradation pathways and the phenylalanine metabolic pathway inhibited protein synthesis and growth in A. japonicus. This study investigated the regulatory response mechanisms in A. japonicus intestinal tissues after exposure to amantadine, providing a theoretical basis for further research on amantadine toxicity.
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spelling pubmed-100535362023-03-30 Amantadine Toxicity in Apostichopus japonicus Revealed by Proteomics Zhao, Junqiang Chen, Jianqiang Tian, Xiuhui Jiang, Lisheng Cui, Qingkui Sun, Yanqing Wu, Ningning Liu, Ge Ding, Yuzhu Wang, Jing Liu, Yongchun Han, Dianfeng Xu, Yingjiang Toxics Article Amantadine exposure can alter biological processes in sea cucumbers, which are an economically important seafood in China. In this study, amantadine toxicity in Apostichopus japonicus was analyzed by oxidative stress and histopathological methods. Quantitative tandem mass tag labeling was used to examine changes in protein contents and metabolic pathways in A. japonicus intestinal tissues after exposure to 100 µg/L amantadine for 96 h. Catalase activity significantly increased from days 1 to 3 of exposure, but it decreased on day 4. Superoxide dismutase and glutathione activities were inhibited throughout the exposure period. Malondialdehyde contents increased on days 1 and 4 but decreased on days 2 and 3. Proteomics analysis revealed 111 differentially expressed proteins in the intestines of A. japonicus after amantadine exposure compared with the control group. An analysis of the involved metabolic pathways showed that the glycolytic and glycogenic pathways may have increased energy production and conversion in A. japonicus after amantadine exposure. The NF-κB, TNF, and IL-17 pathways were likely induced by amantadine exposure, thereby activating NF-κB and triggering intestinal inflammation and apoptosis. Amino acid metabolism analysis showed that the leucine and isoleucine degradation pathways and the phenylalanine metabolic pathway inhibited protein synthesis and growth in A. japonicus. This study investigated the regulatory response mechanisms in A. japonicus intestinal tissues after exposure to amantadine, providing a theoretical basis for further research on amantadine toxicity. MDPI 2023-02-27 /pmc/articles/PMC10053536/ /pubmed/36976991 http://dx.doi.org/10.3390/toxics11030226 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhao, Junqiang
Chen, Jianqiang
Tian, Xiuhui
Jiang, Lisheng
Cui, Qingkui
Sun, Yanqing
Wu, Ningning
Liu, Ge
Ding, Yuzhu
Wang, Jing
Liu, Yongchun
Han, Dianfeng
Xu, Yingjiang
Amantadine Toxicity in Apostichopus japonicus Revealed by Proteomics
title Amantadine Toxicity in Apostichopus japonicus Revealed by Proteomics
title_full Amantadine Toxicity in Apostichopus japonicus Revealed by Proteomics
title_fullStr Amantadine Toxicity in Apostichopus japonicus Revealed by Proteomics
title_full_unstemmed Amantadine Toxicity in Apostichopus japonicus Revealed by Proteomics
title_short Amantadine Toxicity in Apostichopus japonicus Revealed by Proteomics
title_sort amantadine toxicity in apostichopus japonicus revealed by proteomics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10053536/
https://www.ncbi.nlm.nih.gov/pubmed/36976991
http://dx.doi.org/10.3390/toxics11030226
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