Cargando…
Amantadine Toxicity in Apostichopus japonicus Revealed by Proteomics
Amantadine exposure can alter biological processes in sea cucumbers, which are an economically important seafood in China. In this study, amantadine toxicity in Apostichopus japonicus was analyzed by oxidative stress and histopathological methods. Quantitative tandem mass tag labeling was used to ex...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10053536/ https://www.ncbi.nlm.nih.gov/pubmed/36976991 http://dx.doi.org/10.3390/toxics11030226 |
_version_ | 1785015436952207360 |
---|---|
author | Zhao, Junqiang Chen, Jianqiang Tian, Xiuhui Jiang, Lisheng Cui, Qingkui Sun, Yanqing Wu, Ningning Liu, Ge Ding, Yuzhu Wang, Jing Liu, Yongchun Han, Dianfeng Xu, Yingjiang |
author_facet | Zhao, Junqiang Chen, Jianqiang Tian, Xiuhui Jiang, Lisheng Cui, Qingkui Sun, Yanqing Wu, Ningning Liu, Ge Ding, Yuzhu Wang, Jing Liu, Yongchun Han, Dianfeng Xu, Yingjiang |
author_sort | Zhao, Junqiang |
collection | PubMed |
description | Amantadine exposure can alter biological processes in sea cucumbers, which are an economically important seafood in China. In this study, amantadine toxicity in Apostichopus japonicus was analyzed by oxidative stress and histopathological methods. Quantitative tandem mass tag labeling was used to examine changes in protein contents and metabolic pathways in A. japonicus intestinal tissues after exposure to 100 µg/L amantadine for 96 h. Catalase activity significantly increased from days 1 to 3 of exposure, but it decreased on day 4. Superoxide dismutase and glutathione activities were inhibited throughout the exposure period. Malondialdehyde contents increased on days 1 and 4 but decreased on days 2 and 3. Proteomics analysis revealed 111 differentially expressed proteins in the intestines of A. japonicus after amantadine exposure compared with the control group. An analysis of the involved metabolic pathways showed that the glycolytic and glycogenic pathways may have increased energy production and conversion in A. japonicus after amantadine exposure. The NF-κB, TNF, and IL-17 pathways were likely induced by amantadine exposure, thereby activating NF-κB and triggering intestinal inflammation and apoptosis. Amino acid metabolism analysis showed that the leucine and isoleucine degradation pathways and the phenylalanine metabolic pathway inhibited protein synthesis and growth in A. japonicus. This study investigated the regulatory response mechanisms in A. japonicus intestinal tissues after exposure to amantadine, providing a theoretical basis for further research on amantadine toxicity. |
format | Online Article Text |
id | pubmed-10053536 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100535362023-03-30 Amantadine Toxicity in Apostichopus japonicus Revealed by Proteomics Zhao, Junqiang Chen, Jianqiang Tian, Xiuhui Jiang, Lisheng Cui, Qingkui Sun, Yanqing Wu, Ningning Liu, Ge Ding, Yuzhu Wang, Jing Liu, Yongchun Han, Dianfeng Xu, Yingjiang Toxics Article Amantadine exposure can alter biological processes in sea cucumbers, which are an economically important seafood in China. In this study, amantadine toxicity in Apostichopus japonicus was analyzed by oxidative stress and histopathological methods. Quantitative tandem mass tag labeling was used to examine changes in protein contents and metabolic pathways in A. japonicus intestinal tissues after exposure to 100 µg/L amantadine for 96 h. Catalase activity significantly increased from days 1 to 3 of exposure, but it decreased on day 4. Superoxide dismutase and glutathione activities were inhibited throughout the exposure period. Malondialdehyde contents increased on days 1 and 4 but decreased on days 2 and 3. Proteomics analysis revealed 111 differentially expressed proteins in the intestines of A. japonicus after amantadine exposure compared with the control group. An analysis of the involved metabolic pathways showed that the glycolytic and glycogenic pathways may have increased energy production and conversion in A. japonicus after amantadine exposure. The NF-κB, TNF, and IL-17 pathways were likely induced by amantadine exposure, thereby activating NF-κB and triggering intestinal inflammation and apoptosis. Amino acid metabolism analysis showed that the leucine and isoleucine degradation pathways and the phenylalanine metabolic pathway inhibited protein synthesis and growth in A. japonicus. This study investigated the regulatory response mechanisms in A. japonicus intestinal tissues after exposure to amantadine, providing a theoretical basis for further research on amantadine toxicity. MDPI 2023-02-27 /pmc/articles/PMC10053536/ /pubmed/36976991 http://dx.doi.org/10.3390/toxics11030226 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhao, Junqiang Chen, Jianqiang Tian, Xiuhui Jiang, Lisheng Cui, Qingkui Sun, Yanqing Wu, Ningning Liu, Ge Ding, Yuzhu Wang, Jing Liu, Yongchun Han, Dianfeng Xu, Yingjiang Amantadine Toxicity in Apostichopus japonicus Revealed by Proteomics |
title | Amantadine Toxicity in Apostichopus japonicus Revealed by Proteomics |
title_full | Amantadine Toxicity in Apostichopus japonicus Revealed by Proteomics |
title_fullStr | Amantadine Toxicity in Apostichopus japonicus Revealed by Proteomics |
title_full_unstemmed | Amantadine Toxicity in Apostichopus japonicus Revealed by Proteomics |
title_short | Amantadine Toxicity in Apostichopus japonicus Revealed by Proteomics |
title_sort | amantadine toxicity in apostichopus japonicus revealed by proteomics |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10053536/ https://www.ncbi.nlm.nih.gov/pubmed/36976991 http://dx.doi.org/10.3390/toxics11030226 |
work_keys_str_mv | AT zhaojunqiang amantadinetoxicityinapostichopusjaponicusrevealedbyproteomics AT chenjianqiang amantadinetoxicityinapostichopusjaponicusrevealedbyproteomics AT tianxiuhui amantadinetoxicityinapostichopusjaponicusrevealedbyproteomics AT jianglisheng amantadinetoxicityinapostichopusjaponicusrevealedbyproteomics AT cuiqingkui amantadinetoxicityinapostichopusjaponicusrevealedbyproteomics AT sunyanqing amantadinetoxicityinapostichopusjaponicusrevealedbyproteomics AT wuningning amantadinetoxicityinapostichopusjaponicusrevealedbyproteomics AT liuge amantadinetoxicityinapostichopusjaponicusrevealedbyproteomics AT dingyuzhu amantadinetoxicityinapostichopusjaponicusrevealedbyproteomics AT wangjing amantadinetoxicityinapostichopusjaponicusrevealedbyproteomics AT liuyongchun amantadinetoxicityinapostichopusjaponicusrevealedbyproteomics AT handianfeng amantadinetoxicityinapostichopusjaponicusrevealedbyproteomics AT xuyingjiang amantadinetoxicityinapostichopusjaponicusrevealedbyproteomics |