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Do Rutin and Quercetin Retain Their Structure and Radical Scavenging Activity after Exposure to Radiation?
The influence of ionizing radiation on the physicochemical properties of quercetin and rutin in the solid state was studied. Quercetin and rutin were irradiated with the standard recommended radiation dose (25 kGy) according to EN 522 standard. The samples were irradiated by electron beam radiation....
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10053567/ https://www.ncbi.nlm.nih.gov/pubmed/36985686 http://dx.doi.org/10.3390/molecules28062713 |
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author | Rosiak, Natalia Cielecka-Piontek, Judyta Skibiński, Robert Lewandowska, Kornelia Bednarski, Waldemar Zalewski, Przemysław |
author_facet | Rosiak, Natalia Cielecka-Piontek, Judyta Skibiński, Robert Lewandowska, Kornelia Bednarski, Waldemar Zalewski, Przemysław |
author_sort | Rosiak, Natalia |
collection | PubMed |
description | The influence of ionizing radiation on the physicochemical properties of quercetin and rutin in the solid state was studied. Quercetin and rutin were irradiated with the standard recommended radiation dose (25 kGy) according to EN 522 standard. The samples were irradiated by electron beam radiation. EPR studies indicate the formation of a small number of free radicals due to irradiation. Moreover, some radicals recombined with the mean lifetime of 1200 and 93 h, and a stable radical concentration reached only 0.29 and 0.90 ppm for quercetin and rutin, respectively. The performed spectroscopic study (FT-IR) confirmed the radiostability of the flavonoids tested. Chromatographic tests (HPLC, HPLC-MS) showed that irradiation of quercetin and rutin with a 25 kGy dose did not change the physicochemical properties of the tested compounds. Degradation products were not observed. The antioxidant activities were determined by the 2,2-diphenyl-1-pycrylhydrazyl (DPPH) free radical scavenging activity assay, ABTS Radical Scavenging Assay (ABTS), Ferric Reducing Antioxidant Power Assay (FRAP), Cupric Ion Reducing Antioxidant Capacity Assay (CUPRAC). The conducted research confirmed that exposure to ionizing radiation does not change the chemical structure of tested flavonoids and their antioxidant properties. |
format | Online Article Text |
id | pubmed-10053567 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100535672023-03-30 Do Rutin and Quercetin Retain Their Structure and Radical Scavenging Activity after Exposure to Radiation? Rosiak, Natalia Cielecka-Piontek, Judyta Skibiński, Robert Lewandowska, Kornelia Bednarski, Waldemar Zalewski, Przemysław Molecules Article The influence of ionizing radiation on the physicochemical properties of quercetin and rutin in the solid state was studied. Quercetin and rutin were irradiated with the standard recommended radiation dose (25 kGy) according to EN 522 standard. The samples were irradiated by electron beam radiation. EPR studies indicate the formation of a small number of free radicals due to irradiation. Moreover, some radicals recombined with the mean lifetime of 1200 and 93 h, and a stable radical concentration reached only 0.29 and 0.90 ppm for quercetin and rutin, respectively. The performed spectroscopic study (FT-IR) confirmed the radiostability of the flavonoids tested. Chromatographic tests (HPLC, HPLC-MS) showed that irradiation of quercetin and rutin with a 25 kGy dose did not change the physicochemical properties of the tested compounds. Degradation products were not observed. The antioxidant activities were determined by the 2,2-diphenyl-1-pycrylhydrazyl (DPPH) free radical scavenging activity assay, ABTS Radical Scavenging Assay (ABTS), Ferric Reducing Antioxidant Power Assay (FRAP), Cupric Ion Reducing Antioxidant Capacity Assay (CUPRAC). The conducted research confirmed that exposure to ionizing radiation does not change the chemical structure of tested flavonoids and their antioxidant properties. MDPI 2023-03-17 /pmc/articles/PMC10053567/ /pubmed/36985686 http://dx.doi.org/10.3390/molecules28062713 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Rosiak, Natalia Cielecka-Piontek, Judyta Skibiński, Robert Lewandowska, Kornelia Bednarski, Waldemar Zalewski, Przemysław Do Rutin and Quercetin Retain Their Structure and Radical Scavenging Activity after Exposure to Radiation? |
title | Do Rutin and Quercetin Retain Their Structure and Radical Scavenging Activity after Exposure to Radiation? |
title_full | Do Rutin and Quercetin Retain Their Structure and Radical Scavenging Activity after Exposure to Radiation? |
title_fullStr | Do Rutin and Quercetin Retain Their Structure and Radical Scavenging Activity after Exposure to Radiation? |
title_full_unstemmed | Do Rutin and Quercetin Retain Their Structure and Radical Scavenging Activity after Exposure to Radiation? |
title_short | Do Rutin and Quercetin Retain Their Structure and Radical Scavenging Activity after Exposure to Radiation? |
title_sort | do rutin and quercetin retain their structure and radical scavenging activity after exposure to radiation? |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10053567/ https://www.ncbi.nlm.nih.gov/pubmed/36985686 http://dx.doi.org/10.3390/molecules28062713 |
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