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Adaptation of the Porcine Pituitary Transcriptome, Spliceosome and Editome during Early Pregnancy

The physiological mechanisms of the porcine reproduction are relatively well-known. However, transcriptomic changes and the mechanisms accompanying transcription and translation processes in various reproductive organs, as well as their dependence on hormonal status, are still poorly understood. The...

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Autores principales: Makowczenko, Karol G., Jastrzebski, Jan P., Kiezun, Marta, Paukszto, Lukasz, Dobrzyn, Kamil, Smolinska, Nina, Kaminski, Tadeusz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10053595/
https://www.ncbi.nlm.nih.gov/pubmed/36983019
http://dx.doi.org/10.3390/ijms24065946
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author Makowczenko, Karol G.
Jastrzebski, Jan P.
Kiezun, Marta
Paukszto, Lukasz
Dobrzyn, Kamil
Smolinska, Nina
Kaminski, Tadeusz
author_facet Makowczenko, Karol G.
Jastrzebski, Jan P.
Kiezun, Marta
Paukszto, Lukasz
Dobrzyn, Kamil
Smolinska, Nina
Kaminski, Tadeusz
author_sort Makowczenko, Karol G.
collection PubMed
description The physiological mechanisms of the porcine reproduction are relatively well-known. However, transcriptomic changes and the mechanisms accompanying transcription and translation processes in various reproductive organs, as well as their dependence on hormonal status, are still poorly understood. The aim of this study was to gain a principal understanding of alterations within the transcriptome, spliceosome and editome occurring in the pituitary of the domestic pig (Sus scrofa domestica L.), which controls basic physiological processes in the reproductive system. In this investigation, we performed extensive analyses of data obtained by high-throughput sequencing of RNA from the gilts’ pituitary anterior lobes during embryo implantation and the mid-luteal phase of the estrous cycle. During analyses, we obtained detailed information on expression changes of 147 genes and 43 long noncoding RNAs, observed 784 alternative splicing events and also found the occurrence of 8729 allele-specific expression sites and 122 RNA editing events. The expression profiles of the selected 16 phenomena were confirmed by PCR or qPCR techniques. As a final result of functional meta-analysis, we acquired knowledge regarding intracellular pathways that induce changes in the processes accompanying transcription and translation regulation, which may induce modifications in the secretory activity of the porcine adenohypophyseal cells.
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spelling pubmed-100535952023-03-30 Adaptation of the Porcine Pituitary Transcriptome, Spliceosome and Editome during Early Pregnancy Makowczenko, Karol G. Jastrzebski, Jan P. Kiezun, Marta Paukszto, Lukasz Dobrzyn, Kamil Smolinska, Nina Kaminski, Tadeusz Int J Mol Sci Article The physiological mechanisms of the porcine reproduction are relatively well-known. However, transcriptomic changes and the mechanisms accompanying transcription and translation processes in various reproductive organs, as well as their dependence on hormonal status, are still poorly understood. The aim of this study was to gain a principal understanding of alterations within the transcriptome, spliceosome and editome occurring in the pituitary of the domestic pig (Sus scrofa domestica L.), which controls basic physiological processes in the reproductive system. In this investigation, we performed extensive analyses of data obtained by high-throughput sequencing of RNA from the gilts’ pituitary anterior lobes during embryo implantation and the mid-luteal phase of the estrous cycle. During analyses, we obtained detailed information on expression changes of 147 genes and 43 long noncoding RNAs, observed 784 alternative splicing events and also found the occurrence of 8729 allele-specific expression sites and 122 RNA editing events. The expression profiles of the selected 16 phenomena were confirmed by PCR or qPCR techniques. As a final result of functional meta-analysis, we acquired knowledge regarding intracellular pathways that induce changes in the processes accompanying transcription and translation regulation, which may induce modifications in the secretory activity of the porcine adenohypophyseal cells. MDPI 2023-03-21 /pmc/articles/PMC10053595/ /pubmed/36983019 http://dx.doi.org/10.3390/ijms24065946 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Makowczenko, Karol G.
Jastrzebski, Jan P.
Kiezun, Marta
Paukszto, Lukasz
Dobrzyn, Kamil
Smolinska, Nina
Kaminski, Tadeusz
Adaptation of the Porcine Pituitary Transcriptome, Spliceosome and Editome during Early Pregnancy
title Adaptation of the Porcine Pituitary Transcriptome, Spliceosome and Editome during Early Pregnancy
title_full Adaptation of the Porcine Pituitary Transcriptome, Spliceosome and Editome during Early Pregnancy
title_fullStr Adaptation of the Porcine Pituitary Transcriptome, Spliceosome and Editome during Early Pregnancy
title_full_unstemmed Adaptation of the Porcine Pituitary Transcriptome, Spliceosome and Editome during Early Pregnancy
title_short Adaptation of the Porcine Pituitary Transcriptome, Spliceosome and Editome during Early Pregnancy
title_sort adaptation of the porcine pituitary transcriptome, spliceosome and editome during early pregnancy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10053595/
https://www.ncbi.nlm.nih.gov/pubmed/36983019
http://dx.doi.org/10.3390/ijms24065946
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