Cargando…

The Immunosuppressive Niche Established with a Curcumin-Loaded Electrospun Nanofibrous Membrane Promotes Cartilage Regeneration in Immunocompetent Animals

Inflammatory cells mount an immune response against in vitro engineered cartilage implanted into immunocompetent animals, consequently limiting the usage of tissue-engineered cartilage to repair cartilage defects. In this study, curcumin (Cur)—an anti-inflammatory agent—was mixed with poly(lactic-co...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Yu, Cai, Renzhong, Li, Jun, Wu, Xu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10053658/
https://www.ncbi.nlm.nih.gov/pubmed/36984722
http://dx.doi.org/10.3390/membranes13030335
_version_ 1785015466070114304
author Zhang, Yu
Cai, Renzhong
Li, Jun
Wu, Xu
author_facet Zhang, Yu
Cai, Renzhong
Li, Jun
Wu, Xu
author_sort Zhang, Yu
collection PubMed
description Inflammatory cells mount an immune response against in vitro engineered cartilage implanted into immunocompetent animals, consequently limiting the usage of tissue-engineered cartilage to repair cartilage defects. In this study, curcumin (Cur)—an anti-inflammatory agent—was mixed with poly(lactic-co-glycolic acid) (PLGA) to develop a Cur/PLGA nanofibrous membrane with nanoscale pore size and anti-inflammatory properties. Fourier-transform infrared spectroscopy and high-performance liquid chromatography analyses confirmed the successful loading of Cur into the Cur/PLGA nanofibrous membrane. The results of the in vitro assay demonstrated the sustained release kinetics and enhanced stability of Cur in the Cur/PLGA nanofibrous membrane. Western blotting and enzyme-linked immunosorbent assay analyses revealed that the Cur/PLGA nanofibrous membrane significantly downregulated the expression of inflammatory cytokines (IL-1β, IL-6, and TNF-α). A chondrocyte suspension was seeded into a porous PLGA scaffold, and the loaded scaffold was cultured for 3 weeks in vitro to engineer cartilage tissues. The cartilage was packed with the in vitro engineered Cur/PLGA nanofibrous membrane and subcutaneously implanted into rats to generate an immunosuppressive niche. Compared with those in the PLGA-implanted and pure cartilage (without nanofibrous membrane package)-implanted groups, the cartilage was well preserved and the inflammatory response was suppressed in the Cur/PLGA-implanted group at weeks 2 and 4 post-implantation. Thus, this study demonstrated that packaging the cartilage with the Cur/PLGA nanofibrous membrane effectively generated an immunosuppressive niche to protect the cartilage against inflammatory invasion. These findings enable the clinical translation of tissue-engineered cartilage to repair cartilage defects.
format Online
Article
Text
id pubmed-10053658
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-100536582023-03-30 The Immunosuppressive Niche Established with a Curcumin-Loaded Electrospun Nanofibrous Membrane Promotes Cartilage Regeneration in Immunocompetent Animals Zhang, Yu Cai, Renzhong Li, Jun Wu, Xu Membranes (Basel) Article Inflammatory cells mount an immune response against in vitro engineered cartilage implanted into immunocompetent animals, consequently limiting the usage of tissue-engineered cartilage to repair cartilage defects. In this study, curcumin (Cur)—an anti-inflammatory agent—was mixed with poly(lactic-co-glycolic acid) (PLGA) to develop a Cur/PLGA nanofibrous membrane with nanoscale pore size and anti-inflammatory properties. Fourier-transform infrared spectroscopy and high-performance liquid chromatography analyses confirmed the successful loading of Cur into the Cur/PLGA nanofibrous membrane. The results of the in vitro assay demonstrated the sustained release kinetics and enhanced stability of Cur in the Cur/PLGA nanofibrous membrane. Western blotting and enzyme-linked immunosorbent assay analyses revealed that the Cur/PLGA nanofibrous membrane significantly downregulated the expression of inflammatory cytokines (IL-1β, IL-6, and TNF-α). A chondrocyte suspension was seeded into a porous PLGA scaffold, and the loaded scaffold was cultured for 3 weeks in vitro to engineer cartilage tissues. The cartilage was packed with the in vitro engineered Cur/PLGA nanofibrous membrane and subcutaneously implanted into rats to generate an immunosuppressive niche. Compared with those in the PLGA-implanted and pure cartilage (without nanofibrous membrane package)-implanted groups, the cartilage was well preserved and the inflammatory response was suppressed in the Cur/PLGA-implanted group at weeks 2 and 4 post-implantation. Thus, this study demonstrated that packaging the cartilage with the Cur/PLGA nanofibrous membrane effectively generated an immunosuppressive niche to protect the cartilage against inflammatory invasion. These findings enable the clinical translation of tissue-engineered cartilage to repair cartilage defects. MDPI 2023-03-14 /pmc/articles/PMC10053658/ /pubmed/36984722 http://dx.doi.org/10.3390/membranes13030335 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Yu
Cai, Renzhong
Li, Jun
Wu, Xu
The Immunosuppressive Niche Established with a Curcumin-Loaded Electrospun Nanofibrous Membrane Promotes Cartilage Regeneration in Immunocompetent Animals
title The Immunosuppressive Niche Established with a Curcumin-Loaded Electrospun Nanofibrous Membrane Promotes Cartilage Regeneration in Immunocompetent Animals
title_full The Immunosuppressive Niche Established with a Curcumin-Loaded Electrospun Nanofibrous Membrane Promotes Cartilage Regeneration in Immunocompetent Animals
title_fullStr The Immunosuppressive Niche Established with a Curcumin-Loaded Electrospun Nanofibrous Membrane Promotes Cartilage Regeneration in Immunocompetent Animals
title_full_unstemmed The Immunosuppressive Niche Established with a Curcumin-Loaded Electrospun Nanofibrous Membrane Promotes Cartilage Regeneration in Immunocompetent Animals
title_short The Immunosuppressive Niche Established with a Curcumin-Loaded Electrospun Nanofibrous Membrane Promotes Cartilage Regeneration in Immunocompetent Animals
title_sort immunosuppressive niche established with a curcumin-loaded electrospun nanofibrous membrane promotes cartilage regeneration in immunocompetent animals
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10053658/
https://www.ncbi.nlm.nih.gov/pubmed/36984722
http://dx.doi.org/10.3390/membranes13030335
work_keys_str_mv AT zhangyu theimmunosuppressivenicheestablishedwithacurcuminloadedelectrospunnanofibrousmembranepromotescartilageregenerationinimmunocompetentanimals
AT cairenzhong theimmunosuppressivenicheestablishedwithacurcuminloadedelectrospunnanofibrousmembranepromotescartilageregenerationinimmunocompetentanimals
AT lijun theimmunosuppressivenicheestablishedwithacurcuminloadedelectrospunnanofibrousmembranepromotescartilageregenerationinimmunocompetentanimals
AT wuxu theimmunosuppressivenicheestablishedwithacurcuminloadedelectrospunnanofibrousmembranepromotescartilageregenerationinimmunocompetentanimals
AT zhangyu immunosuppressivenicheestablishedwithacurcuminloadedelectrospunnanofibrousmembranepromotescartilageregenerationinimmunocompetentanimals
AT cairenzhong immunosuppressivenicheestablishedwithacurcuminloadedelectrospunnanofibrousmembranepromotescartilageregenerationinimmunocompetentanimals
AT lijun immunosuppressivenicheestablishedwithacurcuminloadedelectrospunnanofibrousmembranepromotescartilageregenerationinimmunocompetentanimals
AT wuxu immunosuppressivenicheestablishedwithacurcuminloadedelectrospunnanofibrousmembranepromotescartilageregenerationinimmunocompetentanimals