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Development of an Inactivated Avian Influenza Virus Vaccine against Circulating H9N2 in Chickens and Ducks

Avian influenza virus (AIV) subtype H9N2 is the most widespread AIV in poultry worldwide, causing great economic losses in the global poultry industry. Chickens and ducks are the major hosts and play essential roles in the transmission and evolution of H9N2 AIV. Vaccines are considered an effective...

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Autores principales: Liu, Yuzhuo, Zhao, Dongmin, Zhang, Jingfeng, Huang, Xinmei, Han, Kaikai, Liu, Qingtao, Yang, Jing, Zhang, Lijiao, Li, Yin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10053827/
https://www.ncbi.nlm.nih.gov/pubmed/36992180
http://dx.doi.org/10.3390/vaccines11030596
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author Liu, Yuzhuo
Zhao, Dongmin
Zhang, Jingfeng
Huang, Xinmei
Han, Kaikai
Liu, Qingtao
Yang, Jing
Zhang, Lijiao
Li, Yin
author_facet Liu, Yuzhuo
Zhao, Dongmin
Zhang, Jingfeng
Huang, Xinmei
Han, Kaikai
Liu, Qingtao
Yang, Jing
Zhang, Lijiao
Li, Yin
author_sort Liu, Yuzhuo
collection PubMed
description Avian influenza virus (AIV) subtype H9N2 is the most widespread AIV in poultry worldwide, causing great economic losses in the global poultry industry. Chickens and ducks are the major hosts and play essential roles in the transmission and evolution of H9N2 AIV. Vaccines are considered an effective strategy for fighting H9N2 infection. However, due to the differences in immune responses to infection, vaccines against H9N2 AIV suitable for use in both chickens and ducks have not been well studied. This study developed an inactivated H9N2 vaccine based on a duck-origin H9N2 AIV and assessed its effectiveness in the laboratory. The results showed that the inactivated H9N2 vaccine elicited significant haemagglutination inhibition (HI) antibodies in both chickens and ducks. Virus challenge experiments revealed that immunization with this vaccine significantly blocked virus shedding after infection by both homogenous and heterologous H9N2 viruses. The vaccine was efficacious in chicken and duck flocks under normal field conditions. We also found that egg-yolk antibodies were produced by laying birds immunized with the inactivated vaccine, and high levels of maternal antibodies were detected in the serum of the offspring. Taken together, our study showed that this inactivated H9N2 vaccine could be extremely favourable for the prevention of H9N2 in both chickens and ducks.
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spelling pubmed-100538272023-03-30 Development of an Inactivated Avian Influenza Virus Vaccine against Circulating H9N2 in Chickens and Ducks Liu, Yuzhuo Zhao, Dongmin Zhang, Jingfeng Huang, Xinmei Han, Kaikai Liu, Qingtao Yang, Jing Zhang, Lijiao Li, Yin Vaccines (Basel) Article Avian influenza virus (AIV) subtype H9N2 is the most widespread AIV in poultry worldwide, causing great economic losses in the global poultry industry. Chickens and ducks are the major hosts and play essential roles in the transmission and evolution of H9N2 AIV. Vaccines are considered an effective strategy for fighting H9N2 infection. However, due to the differences in immune responses to infection, vaccines against H9N2 AIV suitable for use in both chickens and ducks have not been well studied. This study developed an inactivated H9N2 vaccine based on a duck-origin H9N2 AIV and assessed its effectiveness in the laboratory. The results showed that the inactivated H9N2 vaccine elicited significant haemagglutination inhibition (HI) antibodies in both chickens and ducks. Virus challenge experiments revealed that immunization with this vaccine significantly blocked virus shedding after infection by both homogenous and heterologous H9N2 viruses. The vaccine was efficacious in chicken and duck flocks under normal field conditions. We also found that egg-yolk antibodies were produced by laying birds immunized with the inactivated vaccine, and high levels of maternal antibodies were detected in the serum of the offspring. Taken together, our study showed that this inactivated H9N2 vaccine could be extremely favourable for the prevention of H9N2 in both chickens and ducks. MDPI 2023-03-05 /pmc/articles/PMC10053827/ /pubmed/36992180 http://dx.doi.org/10.3390/vaccines11030596 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Yuzhuo
Zhao, Dongmin
Zhang, Jingfeng
Huang, Xinmei
Han, Kaikai
Liu, Qingtao
Yang, Jing
Zhang, Lijiao
Li, Yin
Development of an Inactivated Avian Influenza Virus Vaccine against Circulating H9N2 in Chickens and Ducks
title Development of an Inactivated Avian Influenza Virus Vaccine against Circulating H9N2 in Chickens and Ducks
title_full Development of an Inactivated Avian Influenza Virus Vaccine against Circulating H9N2 in Chickens and Ducks
title_fullStr Development of an Inactivated Avian Influenza Virus Vaccine against Circulating H9N2 in Chickens and Ducks
title_full_unstemmed Development of an Inactivated Avian Influenza Virus Vaccine against Circulating H9N2 in Chickens and Ducks
title_short Development of an Inactivated Avian Influenza Virus Vaccine against Circulating H9N2 in Chickens and Ducks
title_sort development of an inactivated avian influenza virus vaccine against circulating h9n2 in chickens and ducks
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10053827/
https://www.ncbi.nlm.nih.gov/pubmed/36992180
http://dx.doi.org/10.3390/vaccines11030596
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