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Development of an Inactivated Avian Influenza Virus Vaccine against Circulating H9N2 in Chickens and Ducks
Avian influenza virus (AIV) subtype H9N2 is the most widespread AIV in poultry worldwide, causing great economic losses in the global poultry industry. Chickens and ducks are the major hosts and play essential roles in the transmission and evolution of H9N2 AIV. Vaccines are considered an effective...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10053827/ https://www.ncbi.nlm.nih.gov/pubmed/36992180 http://dx.doi.org/10.3390/vaccines11030596 |
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author | Liu, Yuzhuo Zhao, Dongmin Zhang, Jingfeng Huang, Xinmei Han, Kaikai Liu, Qingtao Yang, Jing Zhang, Lijiao Li, Yin |
author_facet | Liu, Yuzhuo Zhao, Dongmin Zhang, Jingfeng Huang, Xinmei Han, Kaikai Liu, Qingtao Yang, Jing Zhang, Lijiao Li, Yin |
author_sort | Liu, Yuzhuo |
collection | PubMed |
description | Avian influenza virus (AIV) subtype H9N2 is the most widespread AIV in poultry worldwide, causing great economic losses in the global poultry industry. Chickens and ducks are the major hosts and play essential roles in the transmission and evolution of H9N2 AIV. Vaccines are considered an effective strategy for fighting H9N2 infection. However, due to the differences in immune responses to infection, vaccines against H9N2 AIV suitable for use in both chickens and ducks have not been well studied. This study developed an inactivated H9N2 vaccine based on a duck-origin H9N2 AIV and assessed its effectiveness in the laboratory. The results showed that the inactivated H9N2 vaccine elicited significant haemagglutination inhibition (HI) antibodies in both chickens and ducks. Virus challenge experiments revealed that immunization with this vaccine significantly blocked virus shedding after infection by both homogenous and heterologous H9N2 viruses. The vaccine was efficacious in chicken and duck flocks under normal field conditions. We also found that egg-yolk antibodies were produced by laying birds immunized with the inactivated vaccine, and high levels of maternal antibodies were detected in the serum of the offspring. Taken together, our study showed that this inactivated H9N2 vaccine could be extremely favourable for the prevention of H9N2 in both chickens and ducks. |
format | Online Article Text |
id | pubmed-10053827 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100538272023-03-30 Development of an Inactivated Avian Influenza Virus Vaccine against Circulating H9N2 in Chickens and Ducks Liu, Yuzhuo Zhao, Dongmin Zhang, Jingfeng Huang, Xinmei Han, Kaikai Liu, Qingtao Yang, Jing Zhang, Lijiao Li, Yin Vaccines (Basel) Article Avian influenza virus (AIV) subtype H9N2 is the most widespread AIV in poultry worldwide, causing great economic losses in the global poultry industry. Chickens and ducks are the major hosts and play essential roles in the transmission and evolution of H9N2 AIV. Vaccines are considered an effective strategy for fighting H9N2 infection. However, due to the differences in immune responses to infection, vaccines against H9N2 AIV suitable for use in both chickens and ducks have not been well studied. This study developed an inactivated H9N2 vaccine based on a duck-origin H9N2 AIV and assessed its effectiveness in the laboratory. The results showed that the inactivated H9N2 vaccine elicited significant haemagglutination inhibition (HI) antibodies in both chickens and ducks. Virus challenge experiments revealed that immunization with this vaccine significantly blocked virus shedding after infection by both homogenous and heterologous H9N2 viruses. The vaccine was efficacious in chicken and duck flocks under normal field conditions. We also found that egg-yolk antibodies were produced by laying birds immunized with the inactivated vaccine, and high levels of maternal antibodies were detected in the serum of the offspring. Taken together, our study showed that this inactivated H9N2 vaccine could be extremely favourable for the prevention of H9N2 in both chickens and ducks. MDPI 2023-03-05 /pmc/articles/PMC10053827/ /pubmed/36992180 http://dx.doi.org/10.3390/vaccines11030596 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Liu, Yuzhuo Zhao, Dongmin Zhang, Jingfeng Huang, Xinmei Han, Kaikai Liu, Qingtao Yang, Jing Zhang, Lijiao Li, Yin Development of an Inactivated Avian Influenza Virus Vaccine against Circulating H9N2 in Chickens and Ducks |
title | Development of an Inactivated Avian Influenza Virus Vaccine against Circulating H9N2 in Chickens and Ducks |
title_full | Development of an Inactivated Avian Influenza Virus Vaccine against Circulating H9N2 in Chickens and Ducks |
title_fullStr | Development of an Inactivated Avian Influenza Virus Vaccine against Circulating H9N2 in Chickens and Ducks |
title_full_unstemmed | Development of an Inactivated Avian Influenza Virus Vaccine against Circulating H9N2 in Chickens and Ducks |
title_short | Development of an Inactivated Avian Influenza Virus Vaccine against Circulating H9N2 in Chickens and Ducks |
title_sort | development of an inactivated avian influenza virus vaccine against circulating h9n2 in chickens and ducks |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10053827/ https://www.ncbi.nlm.nih.gov/pubmed/36992180 http://dx.doi.org/10.3390/vaccines11030596 |
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