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Prospects of Using Gum Arabic Silver Nanoparticles in Toothpaste to Prevent Dental Caries

There is growing interest in the use of green synthesized silver nanoparticles (AgNPs) to control and prevent dental diseases. The incorporation of green synthesized AgNPs into dentifrices to reduce pathogenic oral microbes is motivated by their presumed biocompatibility and broad-spectrum antimicro...

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Autores principales: Ahmed, Omnia Abdelmoneim Khidir, Sibuyi, Nicole Remaliah Samantha, Fadaka, Adewale Oluwaseun, Maboza, Ernest, Olivier, Annette, Madiehe, Abram Madimabe, Meyer, Mervin, Geerts, Greta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10053970/
https://www.ncbi.nlm.nih.gov/pubmed/36986733
http://dx.doi.org/10.3390/pharmaceutics15030871
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author Ahmed, Omnia Abdelmoneim Khidir
Sibuyi, Nicole Remaliah Samantha
Fadaka, Adewale Oluwaseun
Maboza, Ernest
Olivier, Annette
Madiehe, Abram Madimabe
Meyer, Mervin
Geerts, Greta
author_facet Ahmed, Omnia Abdelmoneim Khidir
Sibuyi, Nicole Remaliah Samantha
Fadaka, Adewale Oluwaseun
Maboza, Ernest
Olivier, Annette
Madiehe, Abram Madimabe
Meyer, Mervin
Geerts, Greta
author_sort Ahmed, Omnia Abdelmoneim Khidir
collection PubMed
description There is growing interest in the use of green synthesized silver nanoparticles (AgNPs) to control and prevent dental diseases. The incorporation of green synthesized AgNPs into dentifrices to reduce pathogenic oral microbes is motivated by their presumed biocompatibility and broad-spectrum antimicrobial activity. In the present study, gum arabic AgNPs (GA-AgNPs) were formulated into a toothpaste (TP) using a commercial TP at a non-active concentration, to produce GA-AgNPs_TP. The TP was selected after evaluating the antimicrobial activity of four commercial TPs 1-4 on selected oral microbes using agar disc diffusion and microdilution assays. The less active TP-1 was then used in the formulation of GA-AgNPs_TP-1; thereafter, the antimicrobial activity of GA-AgNPs_0.4g was compared to GA-AgNPs_TP-1. The cytotoxicity of GA-AgNPs_0.4g and GA-AgNPs_TP-1 was also assessed on the buccal mucosa fibroblast (BMF) cells using the MTT assay. The study demonstrated that antimicrobial activity of GA-AgNPs_0.4g was retained after being combined with a sub-lethal or inactive concentration of TP-1. The non-selective antimicrobial activity and cytotoxicity of both GA-AgNPs_0.4g and GA-AgNPs_TP-1 was demonstrated to be time and concentration dependent. These activities were instant, reducing microbial and BMF cell growth in less than one hour of exposure. However, the use of dentifrice commonly takes 2 min and rinsed off thereafter, which could prevent damage to the oral mucosa. Although, GA-AgNPs_TP-1 has a good prospect as a TP or oral healthcare product, more studies are required to further improve the biocompatibility of this formulation.
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spelling pubmed-100539702023-03-30 Prospects of Using Gum Arabic Silver Nanoparticles in Toothpaste to Prevent Dental Caries Ahmed, Omnia Abdelmoneim Khidir Sibuyi, Nicole Remaliah Samantha Fadaka, Adewale Oluwaseun Maboza, Ernest Olivier, Annette Madiehe, Abram Madimabe Meyer, Mervin Geerts, Greta Pharmaceutics Article There is growing interest in the use of green synthesized silver nanoparticles (AgNPs) to control and prevent dental diseases. The incorporation of green synthesized AgNPs into dentifrices to reduce pathogenic oral microbes is motivated by their presumed biocompatibility and broad-spectrum antimicrobial activity. In the present study, gum arabic AgNPs (GA-AgNPs) were formulated into a toothpaste (TP) using a commercial TP at a non-active concentration, to produce GA-AgNPs_TP. The TP was selected after evaluating the antimicrobial activity of four commercial TPs 1-4 on selected oral microbes using agar disc diffusion and microdilution assays. The less active TP-1 was then used in the formulation of GA-AgNPs_TP-1; thereafter, the antimicrobial activity of GA-AgNPs_0.4g was compared to GA-AgNPs_TP-1. The cytotoxicity of GA-AgNPs_0.4g and GA-AgNPs_TP-1 was also assessed on the buccal mucosa fibroblast (BMF) cells using the MTT assay. The study demonstrated that antimicrobial activity of GA-AgNPs_0.4g was retained after being combined with a sub-lethal or inactive concentration of TP-1. The non-selective antimicrobial activity and cytotoxicity of both GA-AgNPs_0.4g and GA-AgNPs_TP-1 was demonstrated to be time and concentration dependent. These activities were instant, reducing microbial and BMF cell growth in less than one hour of exposure. However, the use of dentifrice commonly takes 2 min and rinsed off thereafter, which could prevent damage to the oral mucosa. Although, GA-AgNPs_TP-1 has a good prospect as a TP or oral healthcare product, more studies are required to further improve the biocompatibility of this formulation. MDPI 2023-03-08 /pmc/articles/PMC10053970/ /pubmed/36986733 http://dx.doi.org/10.3390/pharmaceutics15030871 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ahmed, Omnia Abdelmoneim Khidir
Sibuyi, Nicole Remaliah Samantha
Fadaka, Adewale Oluwaseun
Maboza, Ernest
Olivier, Annette
Madiehe, Abram Madimabe
Meyer, Mervin
Geerts, Greta
Prospects of Using Gum Arabic Silver Nanoparticles in Toothpaste to Prevent Dental Caries
title Prospects of Using Gum Arabic Silver Nanoparticles in Toothpaste to Prevent Dental Caries
title_full Prospects of Using Gum Arabic Silver Nanoparticles in Toothpaste to Prevent Dental Caries
title_fullStr Prospects of Using Gum Arabic Silver Nanoparticles in Toothpaste to Prevent Dental Caries
title_full_unstemmed Prospects of Using Gum Arabic Silver Nanoparticles in Toothpaste to Prevent Dental Caries
title_short Prospects of Using Gum Arabic Silver Nanoparticles in Toothpaste to Prevent Dental Caries
title_sort prospects of using gum arabic silver nanoparticles in toothpaste to prevent dental caries
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10053970/
https://www.ncbi.nlm.nih.gov/pubmed/36986733
http://dx.doi.org/10.3390/pharmaceutics15030871
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