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Cervicovaginal Human Papillomavirus Genomes, Microbiota Composition and Cytokine Concentrations in South African Adolescents

The interaction between cervicovaginal virome, bacteriome and genital inflammation has not been extensively investigated. We assessed the vaginal DNA virome from 33 South African adolescents (15–19 years old) using shotgun DNA sequencing of purified virions. We present analyses of eukaryote-infectin...

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Autores principales: Happel, Anna-Ursula, Balle, Christina, Havyarimana, Enock, Brown, Bryan, Maust, Brandon S., Feng, Colin, Yi, Byung H., Gill, Katherine, Bekker, Linda-Gail, Passmore, Jo-Ann S., Jaspan, Heather B., Varsani, Arvind
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10054107/
https://www.ncbi.nlm.nih.gov/pubmed/36992467
http://dx.doi.org/10.3390/v15030758
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author Happel, Anna-Ursula
Balle, Christina
Havyarimana, Enock
Brown, Bryan
Maust, Brandon S.
Feng, Colin
Yi, Byung H.
Gill, Katherine
Bekker, Linda-Gail
Passmore, Jo-Ann S.
Jaspan, Heather B.
Varsani, Arvind
author_facet Happel, Anna-Ursula
Balle, Christina
Havyarimana, Enock
Brown, Bryan
Maust, Brandon S.
Feng, Colin
Yi, Byung H.
Gill, Katherine
Bekker, Linda-Gail
Passmore, Jo-Ann S.
Jaspan, Heather B.
Varsani, Arvind
author_sort Happel, Anna-Ursula
collection PubMed
description The interaction between cervicovaginal virome, bacteriome and genital inflammation has not been extensively investigated. We assessed the vaginal DNA virome from 33 South African adolescents (15–19 years old) using shotgun DNA sequencing of purified virions. We present analyses of eukaryote-infecting DNA viruses, with a focus on human papillomavirus (HPV) genomes and relate these to the vaginal bacterial microbiota (assessed by 16S rRNA gene sequencing) and cytokines (assessed by Luminex). The DNA virome included single-stranded (Anelloviridae, Genomoviridae) and double-stranded DNA viruses (Adenoviridae, Alloherpesviridae, Herpesviridae, Marseilleviridae, Mimiviridae, Polyomaviridae, Poxviridae). We identified 110 unique, complete HPV genomes within two genera (Alphapapillomavirus and Gammapapillomavirus) representing 40 HPV types and 12 species. Of the 40 HPV types identified, 35 showed positive co-infection patterns with at least one other type, mainly HPV-16. HPV-35, a high-risk genotype currently not targeted by available vaccines, was the most prevalent HPV type identified in this cohort. Bacterial taxa commonly associated with bacterial vaginosis also correlated with the presence of HPV. Bacterial vaginosis, rather than HPV, was associated with increased genital inflammation. This study lays the foundation for future work characterizing the vaginal virome and its role in women’s health.
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spelling pubmed-100541072023-03-30 Cervicovaginal Human Papillomavirus Genomes, Microbiota Composition and Cytokine Concentrations in South African Adolescents Happel, Anna-Ursula Balle, Christina Havyarimana, Enock Brown, Bryan Maust, Brandon S. Feng, Colin Yi, Byung H. Gill, Katherine Bekker, Linda-Gail Passmore, Jo-Ann S. Jaspan, Heather B. Varsani, Arvind Viruses Article The interaction between cervicovaginal virome, bacteriome and genital inflammation has not been extensively investigated. We assessed the vaginal DNA virome from 33 South African adolescents (15–19 years old) using shotgun DNA sequencing of purified virions. We present analyses of eukaryote-infecting DNA viruses, with a focus on human papillomavirus (HPV) genomes and relate these to the vaginal bacterial microbiota (assessed by 16S rRNA gene sequencing) and cytokines (assessed by Luminex). The DNA virome included single-stranded (Anelloviridae, Genomoviridae) and double-stranded DNA viruses (Adenoviridae, Alloherpesviridae, Herpesviridae, Marseilleviridae, Mimiviridae, Polyomaviridae, Poxviridae). We identified 110 unique, complete HPV genomes within two genera (Alphapapillomavirus and Gammapapillomavirus) representing 40 HPV types and 12 species. Of the 40 HPV types identified, 35 showed positive co-infection patterns with at least one other type, mainly HPV-16. HPV-35, a high-risk genotype currently not targeted by available vaccines, was the most prevalent HPV type identified in this cohort. Bacterial taxa commonly associated with bacterial vaginosis also correlated with the presence of HPV. Bacterial vaginosis, rather than HPV, was associated with increased genital inflammation. This study lays the foundation for future work characterizing the vaginal virome and its role in women’s health. MDPI 2023-03-15 /pmc/articles/PMC10054107/ /pubmed/36992467 http://dx.doi.org/10.3390/v15030758 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Happel, Anna-Ursula
Balle, Christina
Havyarimana, Enock
Brown, Bryan
Maust, Brandon S.
Feng, Colin
Yi, Byung H.
Gill, Katherine
Bekker, Linda-Gail
Passmore, Jo-Ann S.
Jaspan, Heather B.
Varsani, Arvind
Cervicovaginal Human Papillomavirus Genomes, Microbiota Composition and Cytokine Concentrations in South African Adolescents
title Cervicovaginal Human Papillomavirus Genomes, Microbiota Composition and Cytokine Concentrations in South African Adolescents
title_full Cervicovaginal Human Papillomavirus Genomes, Microbiota Composition and Cytokine Concentrations in South African Adolescents
title_fullStr Cervicovaginal Human Papillomavirus Genomes, Microbiota Composition and Cytokine Concentrations in South African Adolescents
title_full_unstemmed Cervicovaginal Human Papillomavirus Genomes, Microbiota Composition and Cytokine Concentrations in South African Adolescents
title_short Cervicovaginal Human Papillomavirus Genomes, Microbiota Composition and Cytokine Concentrations in South African Adolescents
title_sort cervicovaginal human papillomavirus genomes, microbiota composition and cytokine concentrations in south african adolescents
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10054107/
https://www.ncbi.nlm.nih.gov/pubmed/36992467
http://dx.doi.org/10.3390/v15030758
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