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Identification of m6A-associated LncRNAs as predict factors for the immune infiltration and prognosis of thyroid cancer
OBJECTIVE: This study aims to evaluate the prognostic value of m6A-associated long noncoding RNAs (lncRNAs) and their interaction with tumour microenvironment in thyroid cancer (THCA). METHODS: The clinical and gene expression data of tumours from 502 patients with THCA and 58 adjacent normal tissue...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10054316/ https://www.ncbi.nlm.nih.gov/pubmed/36974635 http://dx.doi.org/10.1080/07853890.2023.2192049 |
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author | Su, Yongcheng Xu, Beibei Li, Jiangquan Shen, Qianwen Lei, Ziyu Ma, Miaomiao Zhang, Fuxing Hu, Tianhui |
author_facet | Su, Yongcheng Xu, Beibei Li, Jiangquan Shen, Qianwen Lei, Ziyu Ma, Miaomiao Zhang, Fuxing Hu, Tianhui |
author_sort | Su, Yongcheng |
collection | PubMed |
description | OBJECTIVE: This study aims to evaluate the prognostic value of m6A-associated long noncoding RNAs (lncRNAs) and their interaction with tumour microenvironment in thyroid cancer (THCA). METHODS: The clinical and gene expression data of tumours from 502 patients with THCA and 58 adjacent normal tissues were retrieved from The Cancer Genome Atlas (TCGA)–THCA dataset. The Pearson test was utilized to identify potential m6A-associated lncRNAs (p < 0.001 and Pearson correlation coefficient > 0.4). Quantitative real-time polymerase chain reaction was performed to verify the expression levels of lncRNAs in tissues. MTT, EdU, colony formation and wound-healing assays were performed to determine the functions of m6A-associated lncRNAs in THCA cell proliferation and metastasis. RESULTS: M6A-associated lncRNAs were identified in three cluster groups. A significant survival difference was found among them, with cluster 1 patients showing worse survival. Moreover, lower immune and estimate scores were correlated to poorer prognosis, and CD8+ T cell and memory CD4+ T cell levels were increased in cluster 1. Cluster 2, with better overall survival, had high expression of PD-L1 and CTLA-4. Eleven of the m6A-associated lncRNAs were screened to establish the risk model, including AC007365.1, AC008555.1, AC040160.1, AC064807.1, AC126773.4, AL023583.1, AL512306.2, EIF2AK3-DT, LINC00667, LYPLAL1-DT and MIR181A2HG. Based on the median risk score, THCA patients were stratified into low-risk and high-risk groups. Overall survival analysis showed a dramatic difference between the two groups. qRCR was performed to verify the expression levels of lncRNA (LYPLAL1-DT, EIF2AK3-DT and MIR181A2HG) in THCA and adjacent normal tissues. Furthermore, functional experiments showed that knockdown of MIR181A2HG obviously inhibited the proliferation and migration of papillary thyroid cancer (PTC) cells in vitro, whereas LYPLAL1-DT overexpression promoted PTC cell proliferation and migration. CONCLUSIONS: Eleven of the m6A-associated lncRNAs were identified as a risk model to predict clinical outcomes and provide a novel and efficient immunotherapeutic strategy for THCA patients. KEY MESSAGES: m6A-associated lncRNAs can be used to predict the clinical outcomes of thyroid cancer patients. An m6A-associated lncRNAs risk model, which can accurately evaluate the immune status and risk stratification in individual thyroid cancer patients, was established. Knockdown/overexpression of representative lncRNAs in the risk model significantly affected the proliferation and migration of papillary thyroid cancer cells. |
format | Online Article Text |
id | pubmed-10054316 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-100543162023-03-30 Identification of m6A-associated LncRNAs as predict factors for the immune infiltration and prognosis of thyroid cancer Su, Yongcheng Xu, Beibei Li, Jiangquan Shen, Qianwen Lei, Ziyu Ma, Miaomiao Zhang, Fuxing Hu, Tianhui Ann Med Original Article OBJECTIVE: This study aims to evaluate the prognostic value of m6A-associated long noncoding RNAs (lncRNAs) and their interaction with tumour microenvironment in thyroid cancer (THCA). METHODS: The clinical and gene expression data of tumours from 502 patients with THCA and 58 adjacent normal tissues were retrieved from The Cancer Genome Atlas (TCGA)–THCA dataset. The Pearson test was utilized to identify potential m6A-associated lncRNAs (p < 0.001 and Pearson correlation coefficient > 0.4). Quantitative real-time polymerase chain reaction was performed to verify the expression levels of lncRNAs in tissues. MTT, EdU, colony formation and wound-healing assays were performed to determine the functions of m6A-associated lncRNAs in THCA cell proliferation and metastasis. RESULTS: M6A-associated lncRNAs were identified in three cluster groups. A significant survival difference was found among them, with cluster 1 patients showing worse survival. Moreover, lower immune and estimate scores were correlated to poorer prognosis, and CD8+ T cell and memory CD4+ T cell levels were increased in cluster 1. Cluster 2, with better overall survival, had high expression of PD-L1 and CTLA-4. Eleven of the m6A-associated lncRNAs were screened to establish the risk model, including AC007365.1, AC008555.1, AC040160.1, AC064807.1, AC126773.4, AL023583.1, AL512306.2, EIF2AK3-DT, LINC00667, LYPLAL1-DT and MIR181A2HG. Based on the median risk score, THCA patients were stratified into low-risk and high-risk groups. Overall survival analysis showed a dramatic difference between the two groups. qRCR was performed to verify the expression levels of lncRNA (LYPLAL1-DT, EIF2AK3-DT and MIR181A2HG) in THCA and adjacent normal tissues. Furthermore, functional experiments showed that knockdown of MIR181A2HG obviously inhibited the proliferation and migration of papillary thyroid cancer (PTC) cells in vitro, whereas LYPLAL1-DT overexpression promoted PTC cell proliferation and migration. CONCLUSIONS: Eleven of the m6A-associated lncRNAs were identified as a risk model to predict clinical outcomes and provide a novel and efficient immunotherapeutic strategy for THCA patients. KEY MESSAGES: m6A-associated lncRNAs can be used to predict the clinical outcomes of thyroid cancer patients. An m6A-associated lncRNAs risk model, which can accurately evaluate the immune status and risk stratification in individual thyroid cancer patients, was established. Knockdown/overexpression of representative lncRNAs in the risk model significantly affected the proliferation and migration of papillary thyroid cancer cells. Taylor & Francis 2023-03-28 /pmc/articles/PMC10054316/ /pubmed/36974635 http://dx.doi.org/10.1080/07853890.2023.2192049 Text en © 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent. |
spellingShingle | Original Article Su, Yongcheng Xu, Beibei Li, Jiangquan Shen, Qianwen Lei, Ziyu Ma, Miaomiao Zhang, Fuxing Hu, Tianhui Identification of m6A-associated LncRNAs as predict factors for the immune infiltration and prognosis of thyroid cancer |
title | Identification of m6A-associated LncRNAs as predict factors for the immune infiltration and prognosis of thyroid cancer |
title_full | Identification of m6A-associated LncRNAs as predict factors for the immune infiltration and prognosis of thyroid cancer |
title_fullStr | Identification of m6A-associated LncRNAs as predict factors for the immune infiltration and prognosis of thyroid cancer |
title_full_unstemmed | Identification of m6A-associated LncRNAs as predict factors for the immune infiltration and prognosis of thyroid cancer |
title_short | Identification of m6A-associated LncRNAs as predict factors for the immune infiltration and prognosis of thyroid cancer |
title_sort | identification of m6a-associated lncrnas as predict factors for the immune infiltration and prognosis of thyroid cancer |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10054316/ https://www.ncbi.nlm.nih.gov/pubmed/36974635 http://dx.doi.org/10.1080/07853890.2023.2192049 |
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