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Beneficial Effects of Hordenine on a Model of Ulcerative Colitis
Hordenine, a phenethylamine alkaloid, is found in a variety of plants and exhibits a broad array of biological activities and pharmacological properties, including anti-inflammatory and anti-fibrotic effects. However, the efficacy and underlying mechanisms of hordenine in treating ulcerative colitis...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10054341/ https://www.ncbi.nlm.nih.gov/pubmed/36985809 http://dx.doi.org/10.3390/molecules28062834 |
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author | Xu, Zhengguang Zhang, Qilian Ding, Ce Wen, Feifei Sun, Fang Liu, Yanzhan Tao, Chunxue Yao, Jing |
author_facet | Xu, Zhengguang Zhang, Qilian Ding, Ce Wen, Feifei Sun, Fang Liu, Yanzhan Tao, Chunxue Yao, Jing |
author_sort | Xu, Zhengguang |
collection | PubMed |
description | Hordenine, a phenethylamine alkaloid, is found in a variety of plants and exhibits a broad array of biological activities and pharmacological properties, including anti-inflammatory and anti-fibrotic effects. However, the efficacy and underlying mechanisms of hordenine in treating ulcerative colitis (UC) remain unclear. To address this, we examined the therapeutic effects of hordenine on dextran sodium sulphate (DSS)-induced UC by comparing disease activity index (DAI), colon length, secretion of inflammatory factors, and degree of colonic histological lesions across diseased mice that were and were not treated with hordenine. We found that hordenine significantly reduced DAI and levels of pro-inflammatory factors, including interleukin (IL)-6, IL-1β, and tumor necrosis factor alpha (TNF-α), and also alleviated colon tissue oedema, colonic lesions, inflammatory cells infiltration and decreased the number of goblet cells. Moreover, in vitro experiments showed that hordenine protected intestinal epithelial barrier function by increasing the expression of tight junction proteins including ZO-1 and occludin, while also promoting the healing of intestinal mucosa. Using immunohistochemistry and western blotting, we demonstrated that hordenine reduced the expression of sphingosine kinase 1 (SPHK1), sphingosine-1-phosphate receptor 1 (S1PR1), and ras-related C3 botulinum toxin substrate 1 (Rac1), and it inhibited the expression of phosphorylated signal transducer and activator of transcription 3 (p-STAT3) in colon tissues. Thus, hordenine appears to be effective in UC treatment owing to pharmacological mechanisms that favor mucosal healing and the inhibition of SPHK-1/S1PR1/STAT3 signaling. |
format | Online Article Text |
id | pubmed-10054341 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100543412023-03-30 Beneficial Effects of Hordenine on a Model of Ulcerative Colitis Xu, Zhengguang Zhang, Qilian Ding, Ce Wen, Feifei Sun, Fang Liu, Yanzhan Tao, Chunxue Yao, Jing Molecules Article Hordenine, a phenethylamine alkaloid, is found in a variety of plants and exhibits a broad array of biological activities and pharmacological properties, including anti-inflammatory and anti-fibrotic effects. However, the efficacy and underlying mechanisms of hordenine in treating ulcerative colitis (UC) remain unclear. To address this, we examined the therapeutic effects of hordenine on dextran sodium sulphate (DSS)-induced UC by comparing disease activity index (DAI), colon length, secretion of inflammatory factors, and degree of colonic histological lesions across diseased mice that were and were not treated with hordenine. We found that hordenine significantly reduced DAI and levels of pro-inflammatory factors, including interleukin (IL)-6, IL-1β, and tumor necrosis factor alpha (TNF-α), and also alleviated colon tissue oedema, colonic lesions, inflammatory cells infiltration and decreased the number of goblet cells. Moreover, in vitro experiments showed that hordenine protected intestinal epithelial barrier function by increasing the expression of tight junction proteins including ZO-1 and occludin, while also promoting the healing of intestinal mucosa. Using immunohistochemistry and western blotting, we demonstrated that hordenine reduced the expression of sphingosine kinase 1 (SPHK1), sphingosine-1-phosphate receptor 1 (S1PR1), and ras-related C3 botulinum toxin substrate 1 (Rac1), and it inhibited the expression of phosphorylated signal transducer and activator of transcription 3 (p-STAT3) in colon tissues. Thus, hordenine appears to be effective in UC treatment owing to pharmacological mechanisms that favor mucosal healing and the inhibition of SPHK-1/S1PR1/STAT3 signaling. MDPI 2023-03-21 /pmc/articles/PMC10054341/ /pubmed/36985809 http://dx.doi.org/10.3390/molecules28062834 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Xu, Zhengguang Zhang, Qilian Ding, Ce Wen, Feifei Sun, Fang Liu, Yanzhan Tao, Chunxue Yao, Jing Beneficial Effects of Hordenine on a Model of Ulcerative Colitis |
title | Beneficial Effects of Hordenine on a Model of Ulcerative Colitis |
title_full | Beneficial Effects of Hordenine on a Model of Ulcerative Colitis |
title_fullStr | Beneficial Effects of Hordenine on a Model of Ulcerative Colitis |
title_full_unstemmed | Beneficial Effects of Hordenine on a Model of Ulcerative Colitis |
title_short | Beneficial Effects of Hordenine on a Model of Ulcerative Colitis |
title_sort | beneficial effects of hordenine on a model of ulcerative colitis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10054341/ https://www.ncbi.nlm.nih.gov/pubmed/36985809 http://dx.doi.org/10.3390/molecules28062834 |
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