Cargando…
Cell Proliferation, Viability, Differentiation, and Apoptosis of Iron Oxide Labeled Stem Cells Transfected with Lipofectamine Assessed by MRI
To assess in vitro and in vivo tracking of iron oxide labeled stem cells transfected by lipofectamine using magnetic resonance imaging (MRI), rat dental pulp stem cells (DPSCs) were characterized, labeled with iron oxide nanoparticles, and then transfected with lipofectamine to facilitate the intern...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10054380/ https://www.ncbi.nlm.nih.gov/pubmed/36983399 http://dx.doi.org/10.3390/jcm12062395 |
_version_ | 1785015656497807360 |
---|---|
author | Jalli, Reza Mehrabani, Davood Zare, Shahrokh Saeedi Moghadam, Mahdi Jamhiri, Iman Manafi, Navid Mehrabani, Golshid Ghabanchi, Janan Razeghian Jahromi, Iman Rasouli-Nia, Aghdass Karimi-Busheri, Feridoun |
author_facet | Jalli, Reza Mehrabani, Davood Zare, Shahrokh Saeedi Moghadam, Mahdi Jamhiri, Iman Manafi, Navid Mehrabani, Golshid Ghabanchi, Janan Razeghian Jahromi, Iman Rasouli-Nia, Aghdass Karimi-Busheri, Feridoun |
author_sort | Jalli, Reza |
collection | PubMed |
description | To assess in vitro and in vivo tracking of iron oxide labeled stem cells transfected by lipofectamine using magnetic resonance imaging (MRI), rat dental pulp stem cells (DPSCs) were characterized, labeled with iron oxide nanoparticles, and then transfected with lipofectamine to facilitate the internalization of these nanoparticles. Cell proliferation, viability, differentiation, and apoptosis were investigated. Prussian blue staining and MRI were used to trace transfected labeled cells. DPSCs were a morphologically spindle shape, adherent to culture plates, and positive for adipogenic and osteogenic inductions. They expressed CD73 and CD90 markers and lacked CD34 and CD45. Iron oxide labeling and transfection with lipofectamine in DPSCs had no toxic impact on viability, proliferation, and differentiation, and did not induce any apoptosis. In vitro and in vivo internalization of iron oxide nanoparticles within DPSCs were confirmed by Prussian blue staining and MRI tracking. Prussian blue staining and MRI tracking in the absence of any toxic effects on cell viability, proliferation, differentiation, and apoptosis were safe and accurate to track DPSCs labeled with iron oxide and transfected with lipofectamine. MRI can be a useful imaging modality when treatment outcome is targeted. |
format | Online Article Text |
id | pubmed-10054380 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100543802023-03-30 Cell Proliferation, Viability, Differentiation, and Apoptosis of Iron Oxide Labeled Stem Cells Transfected with Lipofectamine Assessed by MRI Jalli, Reza Mehrabani, Davood Zare, Shahrokh Saeedi Moghadam, Mahdi Jamhiri, Iman Manafi, Navid Mehrabani, Golshid Ghabanchi, Janan Razeghian Jahromi, Iman Rasouli-Nia, Aghdass Karimi-Busheri, Feridoun J Clin Med Article To assess in vitro and in vivo tracking of iron oxide labeled stem cells transfected by lipofectamine using magnetic resonance imaging (MRI), rat dental pulp stem cells (DPSCs) were characterized, labeled with iron oxide nanoparticles, and then transfected with lipofectamine to facilitate the internalization of these nanoparticles. Cell proliferation, viability, differentiation, and apoptosis were investigated. Prussian blue staining and MRI were used to trace transfected labeled cells. DPSCs were a morphologically spindle shape, adherent to culture plates, and positive for adipogenic and osteogenic inductions. They expressed CD73 and CD90 markers and lacked CD34 and CD45. Iron oxide labeling and transfection with lipofectamine in DPSCs had no toxic impact on viability, proliferation, and differentiation, and did not induce any apoptosis. In vitro and in vivo internalization of iron oxide nanoparticles within DPSCs were confirmed by Prussian blue staining and MRI tracking. Prussian blue staining and MRI tracking in the absence of any toxic effects on cell viability, proliferation, differentiation, and apoptosis were safe and accurate to track DPSCs labeled with iron oxide and transfected with lipofectamine. MRI can be a useful imaging modality when treatment outcome is targeted. MDPI 2023-03-20 /pmc/articles/PMC10054380/ /pubmed/36983399 http://dx.doi.org/10.3390/jcm12062395 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Jalli, Reza Mehrabani, Davood Zare, Shahrokh Saeedi Moghadam, Mahdi Jamhiri, Iman Manafi, Navid Mehrabani, Golshid Ghabanchi, Janan Razeghian Jahromi, Iman Rasouli-Nia, Aghdass Karimi-Busheri, Feridoun Cell Proliferation, Viability, Differentiation, and Apoptosis of Iron Oxide Labeled Stem Cells Transfected with Lipofectamine Assessed by MRI |
title | Cell Proliferation, Viability, Differentiation, and Apoptosis of Iron Oxide Labeled Stem Cells Transfected with Lipofectamine Assessed by MRI |
title_full | Cell Proliferation, Viability, Differentiation, and Apoptosis of Iron Oxide Labeled Stem Cells Transfected with Lipofectamine Assessed by MRI |
title_fullStr | Cell Proliferation, Viability, Differentiation, and Apoptosis of Iron Oxide Labeled Stem Cells Transfected with Lipofectamine Assessed by MRI |
title_full_unstemmed | Cell Proliferation, Viability, Differentiation, and Apoptosis of Iron Oxide Labeled Stem Cells Transfected with Lipofectamine Assessed by MRI |
title_short | Cell Proliferation, Viability, Differentiation, and Apoptosis of Iron Oxide Labeled Stem Cells Transfected with Lipofectamine Assessed by MRI |
title_sort | cell proliferation, viability, differentiation, and apoptosis of iron oxide labeled stem cells transfected with lipofectamine assessed by mri |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10054380/ https://www.ncbi.nlm.nih.gov/pubmed/36983399 http://dx.doi.org/10.3390/jcm12062395 |
work_keys_str_mv | AT jallireza cellproliferationviabilitydifferentiationandapoptosisofironoxidelabeledstemcellstransfectedwithlipofectamineassessedbymri AT mehrabanidavood cellproliferationviabilitydifferentiationandapoptosisofironoxidelabeledstemcellstransfectedwithlipofectamineassessedbymri AT zareshahrokh cellproliferationviabilitydifferentiationandapoptosisofironoxidelabeledstemcellstransfectedwithlipofectamineassessedbymri AT saeedimoghadammahdi cellproliferationviabilitydifferentiationandapoptosisofironoxidelabeledstemcellstransfectedwithlipofectamineassessedbymri AT jamhiriiman cellproliferationviabilitydifferentiationandapoptosisofironoxidelabeledstemcellstransfectedwithlipofectamineassessedbymri AT manafinavid cellproliferationviabilitydifferentiationandapoptosisofironoxidelabeledstemcellstransfectedwithlipofectamineassessedbymri AT mehrabanigolshid cellproliferationviabilitydifferentiationandapoptosisofironoxidelabeledstemcellstransfectedwithlipofectamineassessedbymri AT ghabanchijanan cellproliferationviabilitydifferentiationandapoptosisofironoxidelabeledstemcellstransfectedwithlipofectamineassessedbymri AT razeghianjahromiiman cellproliferationviabilitydifferentiationandapoptosisofironoxidelabeledstemcellstransfectedwithlipofectamineassessedbymri AT rasouliniaaghdass cellproliferationviabilitydifferentiationandapoptosisofironoxidelabeledstemcellstransfectedwithlipofectamineassessedbymri AT karimibusheriferidoun cellproliferationviabilitydifferentiationandapoptosisofironoxidelabeledstemcellstransfectedwithlipofectamineassessedbymri |