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Cell Proliferation, Viability, Differentiation, and Apoptosis of Iron Oxide Labeled Stem Cells Transfected with Lipofectamine Assessed by MRI

To assess in vitro and in vivo tracking of iron oxide labeled stem cells transfected by lipofectamine using magnetic resonance imaging (MRI), rat dental pulp stem cells (DPSCs) were characterized, labeled with iron oxide nanoparticles, and then transfected with lipofectamine to facilitate the intern...

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Autores principales: Jalli, Reza, Mehrabani, Davood, Zare, Shahrokh, Saeedi Moghadam, Mahdi, Jamhiri, Iman, Manafi, Navid, Mehrabani, Golshid, Ghabanchi, Janan, Razeghian Jahromi, Iman, Rasouli-Nia, Aghdass, Karimi-Busheri, Feridoun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10054380/
https://www.ncbi.nlm.nih.gov/pubmed/36983399
http://dx.doi.org/10.3390/jcm12062395
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author Jalli, Reza
Mehrabani, Davood
Zare, Shahrokh
Saeedi Moghadam, Mahdi
Jamhiri, Iman
Manafi, Navid
Mehrabani, Golshid
Ghabanchi, Janan
Razeghian Jahromi, Iman
Rasouli-Nia, Aghdass
Karimi-Busheri, Feridoun
author_facet Jalli, Reza
Mehrabani, Davood
Zare, Shahrokh
Saeedi Moghadam, Mahdi
Jamhiri, Iman
Manafi, Navid
Mehrabani, Golshid
Ghabanchi, Janan
Razeghian Jahromi, Iman
Rasouli-Nia, Aghdass
Karimi-Busheri, Feridoun
author_sort Jalli, Reza
collection PubMed
description To assess in vitro and in vivo tracking of iron oxide labeled stem cells transfected by lipofectamine using magnetic resonance imaging (MRI), rat dental pulp stem cells (DPSCs) were characterized, labeled with iron oxide nanoparticles, and then transfected with lipofectamine to facilitate the internalization of these nanoparticles. Cell proliferation, viability, differentiation, and apoptosis were investigated. Prussian blue staining and MRI were used to trace transfected labeled cells. DPSCs were a morphologically spindle shape, adherent to culture plates, and positive for adipogenic and osteogenic inductions. They expressed CD73 and CD90 markers and lacked CD34 and CD45. Iron oxide labeling and transfection with lipofectamine in DPSCs had no toxic impact on viability, proliferation, and differentiation, and did not induce any apoptosis. In vitro and in vivo internalization of iron oxide nanoparticles within DPSCs were confirmed by Prussian blue staining and MRI tracking. Prussian blue staining and MRI tracking in the absence of any toxic effects on cell viability, proliferation, differentiation, and apoptosis were safe and accurate to track DPSCs labeled with iron oxide and transfected with lipofectamine. MRI can be a useful imaging modality when treatment outcome is targeted.
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spelling pubmed-100543802023-03-30 Cell Proliferation, Viability, Differentiation, and Apoptosis of Iron Oxide Labeled Stem Cells Transfected with Lipofectamine Assessed by MRI Jalli, Reza Mehrabani, Davood Zare, Shahrokh Saeedi Moghadam, Mahdi Jamhiri, Iman Manafi, Navid Mehrabani, Golshid Ghabanchi, Janan Razeghian Jahromi, Iman Rasouli-Nia, Aghdass Karimi-Busheri, Feridoun J Clin Med Article To assess in vitro and in vivo tracking of iron oxide labeled stem cells transfected by lipofectamine using magnetic resonance imaging (MRI), rat dental pulp stem cells (DPSCs) were characterized, labeled with iron oxide nanoparticles, and then transfected with lipofectamine to facilitate the internalization of these nanoparticles. Cell proliferation, viability, differentiation, and apoptosis were investigated. Prussian blue staining and MRI were used to trace transfected labeled cells. DPSCs were a morphologically spindle shape, adherent to culture plates, and positive for adipogenic and osteogenic inductions. They expressed CD73 and CD90 markers and lacked CD34 and CD45. Iron oxide labeling and transfection with lipofectamine in DPSCs had no toxic impact on viability, proliferation, and differentiation, and did not induce any apoptosis. In vitro and in vivo internalization of iron oxide nanoparticles within DPSCs were confirmed by Prussian blue staining and MRI tracking. Prussian blue staining and MRI tracking in the absence of any toxic effects on cell viability, proliferation, differentiation, and apoptosis were safe and accurate to track DPSCs labeled with iron oxide and transfected with lipofectamine. MRI can be a useful imaging modality when treatment outcome is targeted. MDPI 2023-03-20 /pmc/articles/PMC10054380/ /pubmed/36983399 http://dx.doi.org/10.3390/jcm12062395 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jalli, Reza
Mehrabani, Davood
Zare, Shahrokh
Saeedi Moghadam, Mahdi
Jamhiri, Iman
Manafi, Navid
Mehrabani, Golshid
Ghabanchi, Janan
Razeghian Jahromi, Iman
Rasouli-Nia, Aghdass
Karimi-Busheri, Feridoun
Cell Proliferation, Viability, Differentiation, and Apoptosis of Iron Oxide Labeled Stem Cells Transfected with Lipofectamine Assessed by MRI
title Cell Proliferation, Viability, Differentiation, and Apoptosis of Iron Oxide Labeled Stem Cells Transfected with Lipofectamine Assessed by MRI
title_full Cell Proliferation, Viability, Differentiation, and Apoptosis of Iron Oxide Labeled Stem Cells Transfected with Lipofectamine Assessed by MRI
title_fullStr Cell Proliferation, Viability, Differentiation, and Apoptosis of Iron Oxide Labeled Stem Cells Transfected with Lipofectamine Assessed by MRI
title_full_unstemmed Cell Proliferation, Viability, Differentiation, and Apoptosis of Iron Oxide Labeled Stem Cells Transfected with Lipofectamine Assessed by MRI
title_short Cell Proliferation, Viability, Differentiation, and Apoptosis of Iron Oxide Labeled Stem Cells Transfected with Lipofectamine Assessed by MRI
title_sort cell proliferation, viability, differentiation, and apoptosis of iron oxide labeled stem cells transfected with lipofectamine assessed by mri
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10054380/
https://www.ncbi.nlm.nih.gov/pubmed/36983399
http://dx.doi.org/10.3390/jcm12062395
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