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The Growth and Conidiation of Purpureocillium lavendulum Are Co-Regulated by Nitrogen Sources and Histone H3K14 Acetylation

Plant-parasitic nematodes cause severe economic losses to agriculture. As important biocontrol agents, nematophagous fungi evolved the ability to obtain nitrogen sources from nematodes. However, the impact of nitrogen sources on the growth and development of these fungi is largely unknown. In this s...

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Autores principales: Tang, Ping, Han, Jing-Jing, Zhang, Chen-Chen, Tang, Ping-Ping, Qi, Feng-Na, Zhang, Ke-Qin, Liang, Lian-Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10054409/
https://www.ncbi.nlm.nih.gov/pubmed/36983493
http://dx.doi.org/10.3390/jof9030325
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author Tang, Ping
Han, Jing-Jing
Zhang, Chen-Chen
Tang, Ping-Ping
Qi, Feng-Na
Zhang, Ke-Qin
Liang, Lian-Ming
author_facet Tang, Ping
Han, Jing-Jing
Zhang, Chen-Chen
Tang, Ping-Ping
Qi, Feng-Na
Zhang, Ke-Qin
Liang, Lian-Ming
author_sort Tang, Ping
collection PubMed
description Plant-parasitic nematodes cause severe economic losses to agriculture. As important biocontrol agents, nematophagous fungi evolved the ability to obtain nitrogen sources from nematodes. However, the impact of nitrogen sources on the growth and development of these fungi is largely unknown. In this study, we aimed to better understand how nitrogen sources could influence vegetative growth and conidiation through epigenetic regulation in the nematophagous fungus, Purpureocillium lavendulum. Through nutrition screening, we found a phenomenon of the fungus, limited colony extension with a large amount of conidia production when cultured on PDA media, can be altered by adding ammonia nitrate. Characterized by site-directed mutagenesis, the histone H3K14 acetylation was found to be involved in the alternation. Furthermore, the acetyltransferase PlGCN5 was responsible for H3K14 acetylation. Knockout of Plgcn5 severely diminished conidiation in P. lavendulum. Chip-seq showed that H3K14ac distributed in conidiation regulating genes, and genes in the MAPK pathway which may be the downstream targets in the regulation. These findings suggest that histone modification and nitrogen sources coordinated lifestyle regulation in P. lavendulum, providing new insight into the mechanism of growth regulation by nutritional signals for the carnivorous fungus.
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spelling pubmed-100544092023-03-30 The Growth and Conidiation of Purpureocillium lavendulum Are Co-Regulated by Nitrogen Sources and Histone H3K14 Acetylation Tang, Ping Han, Jing-Jing Zhang, Chen-Chen Tang, Ping-Ping Qi, Feng-Na Zhang, Ke-Qin Liang, Lian-Ming J Fungi (Basel) Article Plant-parasitic nematodes cause severe economic losses to agriculture. As important biocontrol agents, nematophagous fungi evolved the ability to obtain nitrogen sources from nematodes. However, the impact of nitrogen sources on the growth and development of these fungi is largely unknown. In this study, we aimed to better understand how nitrogen sources could influence vegetative growth and conidiation through epigenetic regulation in the nematophagous fungus, Purpureocillium lavendulum. Through nutrition screening, we found a phenomenon of the fungus, limited colony extension with a large amount of conidia production when cultured on PDA media, can be altered by adding ammonia nitrate. Characterized by site-directed mutagenesis, the histone H3K14 acetylation was found to be involved in the alternation. Furthermore, the acetyltransferase PlGCN5 was responsible for H3K14 acetylation. Knockout of Plgcn5 severely diminished conidiation in P. lavendulum. Chip-seq showed that H3K14ac distributed in conidiation regulating genes, and genes in the MAPK pathway which may be the downstream targets in the regulation. These findings suggest that histone modification and nitrogen sources coordinated lifestyle regulation in P. lavendulum, providing new insight into the mechanism of growth regulation by nutritional signals for the carnivorous fungus. MDPI 2023-03-06 /pmc/articles/PMC10054409/ /pubmed/36983493 http://dx.doi.org/10.3390/jof9030325 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tang, Ping
Han, Jing-Jing
Zhang, Chen-Chen
Tang, Ping-Ping
Qi, Feng-Na
Zhang, Ke-Qin
Liang, Lian-Ming
The Growth and Conidiation of Purpureocillium lavendulum Are Co-Regulated by Nitrogen Sources and Histone H3K14 Acetylation
title The Growth and Conidiation of Purpureocillium lavendulum Are Co-Regulated by Nitrogen Sources and Histone H3K14 Acetylation
title_full The Growth and Conidiation of Purpureocillium lavendulum Are Co-Regulated by Nitrogen Sources and Histone H3K14 Acetylation
title_fullStr The Growth and Conidiation of Purpureocillium lavendulum Are Co-Regulated by Nitrogen Sources and Histone H3K14 Acetylation
title_full_unstemmed The Growth and Conidiation of Purpureocillium lavendulum Are Co-Regulated by Nitrogen Sources and Histone H3K14 Acetylation
title_short The Growth and Conidiation of Purpureocillium lavendulum Are Co-Regulated by Nitrogen Sources and Histone H3K14 Acetylation
title_sort growth and conidiation of purpureocillium lavendulum are co-regulated by nitrogen sources and histone h3k14 acetylation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10054409/
https://www.ncbi.nlm.nih.gov/pubmed/36983493
http://dx.doi.org/10.3390/jof9030325
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