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Proteomic Analysis of Female Synovial Fluid to Identify Novel Biomarkers for Osteoarthritis

Osteoarthritis (OA) is a highly prevalent degenerative joint condition that disproportionately affects females. The pathophysiology of the disease is not well understood, which makes diagnosis and treatment difficult. Given the physical connection of synovial fluid (SF) with articular tissues, the S...

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Autores principales: Muller, P. Robinson, Lee, Tae Jin, Zhi, Wenbo, Kumar, Sandeep, Vyavahare, Sagar, Sharma, Ashok, Kumar, Vikas, Isales, Carlos M., Hunter, Monte, Fulzele, Sadanand
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10054440/
https://www.ncbi.nlm.nih.gov/pubmed/36983761
http://dx.doi.org/10.3390/life13030605
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author Muller, P. Robinson
Lee, Tae Jin
Zhi, Wenbo
Kumar, Sandeep
Vyavahare, Sagar
Sharma, Ashok
Kumar, Vikas
Isales, Carlos M.
Hunter, Monte
Fulzele, Sadanand
author_facet Muller, P. Robinson
Lee, Tae Jin
Zhi, Wenbo
Kumar, Sandeep
Vyavahare, Sagar
Sharma, Ashok
Kumar, Vikas
Isales, Carlos M.
Hunter, Monte
Fulzele, Sadanand
author_sort Muller, P. Robinson
collection PubMed
description Osteoarthritis (OA) is a highly prevalent degenerative joint condition that disproportionately affects females. The pathophysiology of the disease is not well understood, which makes diagnosis and treatment difficult. Given the physical connection of synovial fluid (SF) with articular tissues, the SF’s composition can reflect relevant biological modifications, and has therefore been a focus of research. Previously, we demonstrated that extracellular vesicles isolated from the synovial fluid of OA patients carry different cargo (protein and miRNA) in a sex-specific manner. Given the increased prevalence and severity of OA in females, this study aims to identify differential protein content within the synovial fluid of female OA and non-osteoarthritic (non-OA) patients. We found that several proteins were differentially expressed in osteoarthritic females compared with age-matched controls. Presenilin, Coagulation Factor X, Lysine-Specific Demethylase 2B, Tenascin C, Leucine-Rich Repeat-Containing Protein 17 fragments, and T-Complex Protein 1 were negatively regulated in the OA group, with PGD Synthase, Tubulointerstitial Nephritis Antigen, and Nuclear Receptor Binding SET Domain Protein 1 positively regulated in the OA group. Database for Annotation, Visualization, and Integrated Discovery (DAVID) and QuickGO analyses established these proteins as significantly involved in many biological, cellular, and molecular processes. In conclusion, the protein content of female synovial fluid is altered in OA patients, which is likely to provide insights into gender-specific pathophysiology.
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spelling pubmed-100544402023-03-30 Proteomic Analysis of Female Synovial Fluid to Identify Novel Biomarkers for Osteoarthritis Muller, P. Robinson Lee, Tae Jin Zhi, Wenbo Kumar, Sandeep Vyavahare, Sagar Sharma, Ashok Kumar, Vikas Isales, Carlos M. Hunter, Monte Fulzele, Sadanand Life (Basel) Article Osteoarthritis (OA) is a highly prevalent degenerative joint condition that disproportionately affects females. The pathophysiology of the disease is not well understood, which makes diagnosis and treatment difficult. Given the physical connection of synovial fluid (SF) with articular tissues, the SF’s composition can reflect relevant biological modifications, and has therefore been a focus of research. Previously, we demonstrated that extracellular vesicles isolated from the synovial fluid of OA patients carry different cargo (protein and miRNA) in a sex-specific manner. Given the increased prevalence and severity of OA in females, this study aims to identify differential protein content within the synovial fluid of female OA and non-osteoarthritic (non-OA) patients. We found that several proteins were differentially expressed in osteoarthritic females compared with age-matched controls. Presenilin, Coagulation Factor X, Lysine-Specific Demethylase 2B, Tenascin C, Leucine-Rich Repeat-Containing Protein 17 fragments, and T-Complex Protein 1 were negatively regulated in the OA group, with PGD Synthase, Tubulointerstitial Nephritis Antigen, and Nuclear Receptor Binding SET Domain Protein 1 positively regulated in the OA group. Database for Annotation, Visualization, and Integrated Discovery (DAVID) and QuickGO analyses established these proteins as significantly involved in many biological, cellular, and molecular processes. In conclusion, the protein content of female synovial fluid is altered in OA patients, which is likely to provide insights into gender-specific pathophysiology. MDPI 2023-02-22 /pmc/articles/PMC10054440/ /pubmed/36983761 http://dx.doi.org/10.3390/life13030605 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Muller, P. Robinson
Lee, Tae Jin
Zhi, Wenbo
Kumar, Sandeep
Vyavahare, Sagar
Sharma, Ashok
Kumar, Vikas
Isales, Carlos M.
Hunter, Monte
Fulzele, Sadanand
Proteomic Analysis of Female Synovial Fluid to Identify Novel Biomarkers for Osteoarthritis
title Proteomic Analysis of Female Synovial Fluid to Identify Novel Biomarkers for Osteoarthritis
title_full Proteomic Analysis of Female Synovial Fluid to Identify Novel Biomarkers for Osteoarthritis
title_fullStr Proteomic Analysis of Female Synovial Fluid to Identify Novel Biomarkers for Osteoarthritis
title_full_unstemmed Proteomic Analysis of Female Synovial Fluid to Identify Novel Biomarkers for Osteoarthritis
title_short Proteomic Analysis of Female Synovial Fluid to Identify Novel Biomarkers for Osteoarthritis
title_sort proteomic analysis of female synovial fluid to identify novel biomarkers for osteoarthritis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10054440/
https://www.ncbi.nlm.nih.gov/pubmed/36983761
http://dx.doi.org/10.3390/life13030605
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