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Proteomic Analysis of Female Synovial Fluid to Identify Novel Biomarkers for Osteoarthritis
Osteoarthritis (OA) is a highly prevalent degenerative joint condition that disproportionately affects females. The pathophysiology of the disease is not well understood, which makes diagnosis and treatment difficult. Given the physical connection of synovial fluid (SF) with articular tissues, the S...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10054440/ https://www.ncbi.nlm.nih.gov/pubmed/36983761 http://dx.doi.org/10.3390/life13030605 |
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author | Muller, P. Robinson Lee, Tae Jin Zhi, Wenbo Kumar, Sandeep Vyavahare, Sagar Sharma, Ashok Kumar, Vikas Isales, Carlos M. Hunter, Monte Fulzele, Sadanand |
author_facet | Muller, P. Robinson Lee, Tae Jin Zhi, Wenbo Kumar, Sandeep Vyavahare, Sagar Sharma, Ashok Kumar, Vikas Isales, Carlos M. Hunter, Monte Fulzele, Sadanand |
author_sort | Muller, P. Robinson |
collection | PubMed |
description | Osteoarthritis (OA) is a highly prevalent degenerative joint condition that disproportionately affects females. The pathophysiology of the disease is not well understood, which makes diagnosis and treatment difficult. Given the physical connection of synovial fluid (SF) with articular tissues, the SF’s composition can reflect relevant biological modifications, and has therefore been a focus of research. Previously, we demonstrated that extracellular vesicles isolated from the synovial fluid of OA patients carry different cargo (protein and miRNA) in a sex-specific manner. Given the increased prevalence and severity of OA in females, this study aims to identify differential protein content within the synovial fluid of female OA and non-osteoarthritic (non-OA) patients. We found that several proteins were differentially expressed in osteoarthritic females compared with age-matched controls. Presenilin, Coagulation Factor X, Lysine-Specific Demethylase 2B, Tenascin C, Leucine-Rich Repeat-Containing Protein 17 fragments, and T-Complex Protein 1 were negatively regulated in the OA group, with PGD Synthase, Tubulointerstitial Nephritis Antigen, and Nuclear Receptor Binding SET Domain Protein 1 positively regulated in the OA group. Database for Annotation, Visualization, and Integrated Discovery (DAVID) and QuickGO analyses established these proteins as significantly involved in many biological, cellular, and molecular processes. In conclusion, the protein content of female synovial fluid is altered in OA patients, which is likely to provide insights into gender-specific pathophysiology. |
format | Online Article Text |
id | pubmed-10054440 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100544402023-03-30 Proteomic Analysis of Female Synovial Fluid to Identify Novel Biomarkers for Osteoarthritis Muller, P. Robinson Lee, Tae Jin Zhi, Wenbo Kumar, Sandeep Vyavahare, Sagar Sharma, Ashok Kumar, Vikas Isales, Carlos M. Hunter, Monte Fulzele, Sadanand Life (Basel) Article Osteoarthritis (OA) is a highly prevalent degenerative joint condition that disproportionately affects females. The pathophysiology of the disease is not well understood, which makes diagnosis and treatment difficult. Given the physical connection of synovial fluid (SF) with articular tissues, the SF’s composition can reflect relevant biological modifications, and has therefore been a focus of research. Previously, we demonstrated that extracellular vesicles isolated from the synovial fluid of OA patients carry different cargo (protein and miRNA) in a sex-specific manner. Given the increased prevalence and severity of OA in females, this study aims to identify differential protein content within the synovial fluid of female OA and non-osteoarthritic (non-OA) patients. We found that several proteins were differentially expressed in osteoarthritic females compared with age-matched controls. Presenilin, Coagulation Factor X, Lysine-Specific Demethylase 2B, Tenascin C, Leucine-Rich Repeat-Containing Protein 17 fragments, and T-Complex Protein 1 were negatively regulated in the OA group, with PGD Synthase, Tubulointerstitial Nephritis Antigen, and Nuclear Receptor Binding SET Domain Protein 1 positively regulated in the OA group. Database for Annotation, Visualization, and Integrated Discovery (DAVID) and QuickGO analyses established these proteins as significantly involved in many biological, cellular, and molecular processes. In conclusion, the protein content of female synovial fluid is altered in OA patients, which is likely to provide insights into gender-specific pathophysiology. MDPI 2023-02-22 /pmc/articles/PMC10054440/ /pubmed/36983761 http://dx.doi.org/10.3390/life13030605 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Muller, P. Robinson Lee, Tae Jin Zhi, Wenbo Kumar, Sandeep Vyavahare, Sagar Sharma, Ashok Kumar, Vikas Isales, Carlos M. Hunter, Monte Fulzele, Sadanand Proteomic Analysis of Female Synovial Fluid to Identify Novel Biomarkers for Osteoarthritis |
title | Proteomic Analysis of Female Synovial Fluid to Identify Novel Biomarkers for Osteoarthritis |
title_full | Proteomic Analysis of Female Synovial Fluid to Identify Novel Biomarkers for Osteoarthritis |
title_fullStr | Proteomic Analysis of Female Synovial Fluid to Identify Novel Biomarkers for Osteoarthritis |
title_full_unstemmed | Proteomic Analysis of Female Synovial Fluid to Identify Novel Biomarkers for Osteoarthritis |
title_short | Proteomic Analysis of Female Synovial Fluid to Identify Novel Biomarkers for Osteoarthritis |
title_sort | proteomic analysis of female synovial fluid to identify novel biomarkers for osteoarthritis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10054440/ https://www.ncbi.nlm.nih.gov/pubmed/36983761 http://dx.doi.org/10.3390/life13030605 |
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