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Investigating Biochemical and Histopathological Responses between Raspberries and Aculeastrum americanum
Late leaf rust is a fungal disease in raspberries caused by Aculeastrum americanum (Farl.) M. Scholler U. Braun (syn. Thekopsora americana (Farl.) Aime McTaggart) leading to early defoliation and yield losses. Red raspberries (Rubus idaeus L.) are susceptible to this pathogen, although this suscepti...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10054533/ https://www.ncbi.nlm.nih.gov/pubmed/36983505 http://dx.doi.org/10.3390/jof9030337 |
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author | Dias, Márcia Gonçalves Spósito, Marcel Bellato Tessmer, Magda Andréia Appezzato-da-Glória, Beatriz |
author_facet | Dias, Márcia Gonçalves Spósito, Marcel Bellato Tessmer, Magda Andréia Appezzato-da-Glória, Beatriz |
author_sort | Dias, Márcia Gonçalves |
collection | PubMed |
description | Late leaf rust is a fungal disease in raspberries caused by Aculeastrum americanum (Farl.) M. Scholler U. Braun (syn. Thekopsora americana (Farl.) Aime McTaggart) leading to early defoliation and yield losses. Red raspberries (Rubus idaeus L.) are susceptible to this pathogen, although this susceptibility varies among cultivars. In contrast, black raspberries were previously reported to be more resistant (Rubus occidentalis L.) and immune (Rubus niveus Thunb.) to this pathogen, raising their importance in plant breeding programs. However, what features make them respond differently to the same pathogen? In this study, we characterize for the first time the pre- and post-formed structural and biochemical defense mechanisms of R. idaeus cv. Autumn Bliss, R. occidentalis and R. niveus. Ultrastructural and histopathological analyses were used to uncover the interactions between these raspberries and A. americanum. The ultrastructural results indicate that the pathogen germinates on both leaf surfaces but can only form appressoria on the stomata. Although the three raspberry species were infected and colonized by A. americanum, a clear difference in susceptibility was observed between them. A compact mesophyll, pre- and post-formed phenolic compounds, and post-formed pectic compounds were the main plant defense mechanisms against fungal colonization. These findings provide new information about raspberries’ defense mechanisms in response to A. americanum and elucidate the interactions occurring in these pathosystems. |
format | Online Article Text |
id | pubmed-10054533 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100545332023-03-30 Investigating Biochemical and Histopathological Responses between Raspberries and Aculeastrum americanum Dias, Márcia Gonçalves Spósito, Marcel Bellato Tessmer, Magda Andréia Appezzato-da-Glória, Beatriz J Fungi (Basel) Article Late leaf rust is a fungal disease in raspberries caused by Aculeastrum americanum (Farl.) M. Scholler U. Braun (syn. Thekopsora americana (Farl.) Aime McTaggart) leading to early defoliation and yield losses. Red raspberries (Rubus idaeus L.) are susceptible to this pathogen, although this susceptibility varies among cultivars. In contrast, black raspberries were previously reported to be more resistant (Rubus occidentalis L.) and immune (Rubus niveus Thunb.) to this pathogen, raising their importance in plant breeding programs. However, what features make them respond differently to the same pathogen? In this study, we characterize for the first time the pre- and post-formed structural and biochemical defense mechanisms of R. idaeus cv. Autumn Bliss, R. occidentalis and R. niveus. Ultrastructural and histopathological analyses were used to uncover the interactions between these raspberries and A. americanum. The ultrastructural results indicate that the pathogen germinates on both leaf surfaces but can only form appressoria on the stomata. Although the three raspberry species were infected and colonized by A. americanum, a clear difference in susceptibility was observed between them. A compact mesophyll, pre- and post-formed phenolic compounds, and post-formed pectic compounds were the main plant defense mechanisms against fungal colonization. These findings provide new information about raspberries’ defense mechanisms in response to A. americanum and elucidate the interactions occurring in these pathosystems. MDPI 2023-03-09 /pmc/articles/PMC10054533/ /pubmed/36983505 http://dx.doi.org/10.3390/jof9030337 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Dias, Márcia Gonçalves Spósito, Marcel Bellato Tessmer, Magda Andréia Appezzato-da-Glória, Beatriz Investigating Biochemical and Histopathological Responses between Raspberries and Aculeastrum americanum |
title | Investigating Biochemical and Histopathological Responses between Raspberries and Aculeastrum americanum |
title_full | Investigating Biochemical and Histopathological Responses between Raspberries and Aculeastrum americanum |
title_fullStr | Investigating Biochemical and Histopathological Responses between Raspberries and Aculeastrum americanum |
title_full_unstemmed | Investigating Biochemical and Histopathological Responses between Raspberries and Aculeastrum americanum |
title_short | Investigating Biochemical and Histopathological Responses between Raspberries and Aculeastrum americanum |
title_sort | investigating biochemical and histopathological responses between raspberries and aculeastrum americanum |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10054533/ https://www.ncbi.nlm.nih.gov/pubmed/36983505 http://dx.doi.org/10.3390/jof9030337 |
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