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Antifungal Action of Herbal Plants’ Glycolic Extracts against Candida Species

Candida spp. cause fungal infection that affects patients’ oral health. This study aimed to evaluate the isolated and synergistic antifungal effect of Rosa centifolia L., Curcuma longa L., Rosmarinus officinalis L., and Punica granatum L. glycolic extracts against Candida albicans, Candida dublinien...

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Autores principales: Meccatti, Vanessa Marques, Santos, Lana Ferreira, de Carvalho, Lara Steffany, Souza, Clara Bulhões, Carvalho, Cláudio Antonio Talge, Marcucci, Maria Cristina, Abu Hasna, Amjad, de Oliveira, Luciane Dias
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10054556/
https://www.ncbi.nlm.nih.gov/pubmed/36985829
http://dx.doi.org/10.3390/molecules28062857
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author Meccatti, Vanessa Marques
Santos, Lana Ferreira
de Carvalho, Lara Steffany
Souza, Clara Bulhões
Carvalho, Cláudio Antonio Talge
Marcucci, Maria Cristina
Abu Hasna, Amjad
de Oliveira, Luciane Dias
author_facet Meccatti, Vanessa Marques
Santos, Lana Ferreira
de Carvalho, Lara Steffany
Souza, Clara Bulhões
Carvalho, Cláudio Antonio Talge
Marcucci, Maria Cristina
Abu Hasna, Amjad
de Oliveira, Luciane Dias
author_sort Meccatti, Vanessa Marques
collection PubMed
description Candida spp. cause fungal infection that affects patients’ oral health. This study aimed to evaluate the isolated and synergistic antifungal effect of Rosa centifolia L., Curcuma longa L., Rosmarinus officinalis L., and Punica granatum L. glycolic extracts against Candida albicans, Candida dubliniensis, Candida tropicalis, and Candida krusei planktonic and biofilm forms. The plant extracts were chemically characterized and the main compounds were quantified by high-performance liquid chromatography (HPLC-DAD) analysis. The minimum inhibitory and minimum fungicidal concentrations of the extracts were determined, and antibiofilm activity was evaluated by MTT assay. Data were analyzed by one-way ANOVA and Tukey’s tests, and by Kruskal–Wallis and Dunn’s tests, considering a significance level of 5%. The main compounds identified in each of the extracts were: p-coumaric acid (2153.22 μg/100 mL) in the rosemary extract, gallotannins (4318.31 μg/100 mL) in the pomegranate extract, quercetin derivatives (3316.50 μg/100 mL) in the extract of white roses, and curcumin (135.09 μg/100 mL) in the turmeric extract. The combination of R. centifolia and C. longa glycolic extracts was effective against C. albicans, C. dubliniensis, and C. tropicalis biofilms over different periods (p < 0.05). The combination of R. officinalis and P. granatum glycolic extracts was effective against C. albicans and C. krusei biofilms after 30 min, and against C. tropicalis after 24 h, with all combinations showing an average reduction of 50% in cell viability (p < 0.05). In conclusion, the combined plant extracts have antifungal and antibiofilm action against Candida spp. in different concentrations and times of action.
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spelling pubmed-100545562023-03-30 Antifungal Action of Herbal Plants’ Glycolic Extracts against Candida Species Meccatti, Vanessa Marques Santos, Lana Ferreira de Carvalho, Lara Steffany Souza, Clara Bulhões Carvalho, Cláudio Antonio Talge Marcucci, Maria Cristina Abu Hasna, Amjad de Oliveira, Luciane Dias Molecules Article Candida spp. cause fungal infection that affects patients’ oral health. This study aimed to evaluate the isolated and synergistic antifungal effect of Rosa centifolia L., Curcuma longa L., Rosmarinus officinalis L., and Punica granatum L. glycolic extracts against Candida albicans, Candida dubliniensis, Candida tropicalis, and Candida krusei planktonic and biofilm forms. The plant extracts were chemically characterized and the main compounds were quantified by high-performance liquid chromatography (HPLC-DAD) analysis. The minimum inhibitory and minimum fungicidal concentrations of the extracts were determined, and antibiofilm activity was evaluated by MTT assay. Data were analyzed by one-way ANOVA and Tukey’s tests, and by Kruskal–Wallis and Dunn’s tests, considering a significance level of 5%. The main compounds identified in each of the extracts were: p-coumaric acid (2153.22 μg/100 mL) in the rosemary extract, gallotannins (4318.31 μg/100 mL) in the pomegranate extract, quercetin derivatives (3316.50 μg/100 mL) in the extract of white roses, and curcumin (135.09 μg/100 mL) in the turmeric extract. The combination of R. centifolia and C. longa glycolic extracts was effective against C. albicans, C. dubliniensis, and C. tropicalis biofilms over different periods (p < 0.05). The combination of R. officinalis and P. granatum glycolic extracts was effective against C. albicans and C. krusei biofilms after 30 min, and against C. tropicalis after 24 h, with all combinations showing an average reduction of 50% in cell viability (p < 0.05). In conclusion, the combined plant extracts have antifungal and antibiofilm action against Candida spp. in different concentrations and times of action. MDPI 2023-03-22 /pmc/articles/PMC10054556/ /pubmed/36985829 http://dx.doi.org/10.3390/molecules28062857 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Meccatti, Vanessa Marques
Santos, Lana Ferreira
de Carvalho, Lara Steffany
Souza, Clara Bulhões
Carvalho, Cláudio Antonio Talge
Marcucci, Maria Cristina
Abu Hasna, Amjad
de Oliveira, Luciane Dias
Antifungal Action of Herbal Plants’ Glycolic Extracts against Candida Species
title Antifungal Action of Herbal Plants’ Glycolic Extracts against Candida Species
title_full Antifungal Action of Herbal Plants’ Glycolic Extracts against Candida Species
title_fullStr Antifungal Action of Herbal Plants’ Glycolic Extracts against Candida Species
title_full_unstemmed Antifungal Action of Herbal Plants’ Glycolic Extracts against Candida Species
title_short Antifungal Action of Herbal Plants’ Glycolic Extracts against Candida Species
title_sort antifungal action of herbal plants’ glycolic extracts against candida species
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10054556/
https://www.ncbi.nlm.nih.gov/pubmed/36985829
http://dx.doi.org/10.3390/molecules28062857
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