Cargando…
A Multifunctional Trypsin Protease Inhibitor from Yellow Bell Pepper Seeds: Uncovering Its Dual Antifungal and Hypoglycemic Properties
Fungal infections are a growing public health concern worldwide and the emergence of antifungal resistance has limited the number of therapeutic options. Therefore, developing novel strategies for identifying and developing new antifungal compounds is an active area of research in the pharmaceutical...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10054557/ https://www.ncbi.nlm.nih.gov/pubmed/36986642 http://dx.doi.org/10.3390/pharmaceutics15030781 |
_version_ | 1785015699634126848 |
---|---|
author | Cotabarren, Juliana Ozón, Brenda Claver, Santiago Geier, Florencia Rossotti, Martina Garcia-Pardo, Javier Obregón, Walter David |
author_facet | Cotabarren, Juliana Ozón, Brenda Claver, Santiago Geier, Florencia Rossotti, Martina Garcia-Pardo, Javier Obregón, Walter David |
author_sort | Cotabarren, Juliana |
collection | PubMed |
description | Fungal infections are a growing public health concern worldwide and the emergence of antifungal resistance has limited the number of therapeutic options. Therefore, developing novel strategies for identifying and developing new antifungal compounds is an active area of research in the pharmaceutical industry. In this study, we purified and characterized a trypsin protease inhibitor obtained from Yellow Bell Pepper (Capsicum annuum L.) seeds. The inhibitor not only showed potent and specific activity against the pathogenic fungus Candida albicans, but was also found to be non-toxic against human cells. Furthermore, this inhibitor is unique in that it also inhibits α-1,4-glucosidase, positioning it as one of the first plant-derived protease inhibitors with dual biological activity. This exciting discovery opens new avenues for the development of this inhibitor as a promising antifungal agent and highlights the potential of plant-derived protease inhibitors as a rich source for the discovery of novel multifunctional bioactive molecules. |
format | Online Article Text |
id | pubmed-10054557 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100545572023-03-30 A Multifunctional Trypsin Protease Inhibitor from Yellow Bell Pepper Seeds: Uncovering Its Dual Antifungal and Hypoglycemic Properties Cotabarren, Juliana Ozón, Brenda Claver, Santiago Geier, Florencia Rossotti, Martina Garcia-Pardo, Javier Obregón, Walter David Pharmaceutics Article Fungal infections are a growing public health concern worldwide and the emergence of antifungal resistance has limited the number of therapeutic options. Therefore, developing novel strategies for identifying and developing new antifungal compounds is an active area of research in the pharmaceutical industry. In this study, we purified and characterized a trypsin protease inhibitor obtained from Yellow Bell Pepper (Capsicum annuum L.) seeds. The inhibitor not only showed potent and specific activity against the pathogenic fungus Candida albicans, but was also found to be non-toxic against human cells. Furthermore, this inhibitor is unique in that it also inhibits α-1,4-glucosidase, positioning it as one of the first plant-derived protease inhibitors with dual biological activity. This exciting discovery opens new avenues for the development of this inhibitor as a promising antifungal agent and highlights the potential of plant-derived protease inhibitors as a rich source for the discovery of novel multifunctional bioactive molecules. MDPI 2023-02-27 /pmc/articles/PMC10054557/ /pubmed/36986642 http://dx.doi.org/10.3390/pharmaceutics15030781 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cotabarren, Juliana Ozón, Brenda Claver, Santiago Geier, Florencia Rossotti, Martina Garcia-Pardo, Javier Obregón, Walter David A Multifunctional Trypsin Protease Inhibitor from Yellow Bell Pepper Seeds: Uncovering Its Dual Antifungal and Hypoglycemic Properties |
title | A Multifunctional Trypsin Protease Inhibitor from Yellow Bell Pepper Seeds: Uncovering Its Dual Antifungal and Hypoglycemic Properties |
title_full | A Multifunctional Trypsin Protease Inhibitor from Yellow Bell Pepper Seeds: Uncovering Its Dual Antifungal and Hypoglycemic Properties |
title_fullStr | A Multifunctional Trypsin Protease Inhibitor from Yellow Bell Pepper Seeds: Uncovering Its Dual Antifungal and Hypoglycemic Properties |
title_full_unstemmed | A Multifunctional Trypsin Protease Inhibitor from Yellow Bell Pepper Seeds: Uncovering Its Dual Antifungal and Hypoglycemic Properties |
title_short | A Multifunctional Trypsin Protease Inhibitor from Yellow Bell Pepper Seeds: Uncovering Its Dual Antifungal and Hypoglycemic Properties |
title_sort | multifunctional trypsin protease inhibitor from yellow bell pepper seeds: uncovering its dual antifungal and hypoglycemic properties |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10054557/ https://www.ncbi.nlm.nih.gov/pubmed/36986642 http://dx.doi.org/10.3390/pharmaceutics15030781 |
work_keys_str_mv | AT cotabarrenjuliana amultifunctionaltrypsinproteaseinhibitorfromyellowbellpepperseedsuncoveringitsdualantifungalandhypoglycemicproperties AT ozonbrenda amultifunctionaltrypsinproteaseinhibitorfromyellowbellpepperseedsuncoveringitsdualantifungalandhypoglycemicproperties AT claversantiago amultifunctionaltrypsinproteaseinhibitorfromyellowbellpepperseedsuncoveringitsdualantifungalandhypoglycemicproperties AT geierflorencia amultifunctionaltrypsinproteaseinhibitorfromyellowbellpepperseedsuncoveringitsdualantifungalandhypoglycemicproperties AT rossottimartina amultifunctionaltrypsinproteaseinhibitorfromyellowbellpepperseedsuncoveringitsdualantifungalandhypoglycemicproperties AT garciapardojavier amultifunctionaltrypsinproteaseinhibitorfromyellowbellpepperseedsuncoveringitsdualantifungalandhypoglycemicproperties AT obregonwalterdavid amultifunctionaltrypsinproteaseinhibitorfromyellowbellpepperseedsuncoveringitsdualantifungalandhypoglycemicproperties AT cotabarrenjuliana multifunctionaltrypsinproteaseinhibitorfromyellowbellpepperseedsuncoveringitsdualantifungalandhypoglycemicproperties AT ozonbrenda multifunctionaltrypsinproteaseinhibitorfromyellowbellpepperseedsuncoveringitsdualantifungalandhypoglycemicproperties AT claversantiago multifunctionaltrypsinproteaseinhibitorfromyellowbellpepperseedsuncoveringitsdualantifungalandhypoglycemicproperties AT geierflorencia multifunctionaltrypsinproteaseinhibitorfromyellowbellpepperseedsuncoveringitsdualantifungalandhypoglycemicproperties AT rossottimartina multifunctionaltrypsinproteaseinhibitorfromyellowbellpepperseedsuncoveringitsdualantifungalandhypoglycemicproperties AT garciapardojavier multifunctionaltrypsinproteaseinhibitorfromyellowbellpepperseedsuncoveringitsdualantifungalandhypoglycemicproperties AT obregonwalterdavid multifunctionaltrypsinproteaseinhibitorfromyellowbellpepperseedsuncoveringitsdualantifungalandhypoglycemicproperties |