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A Multifunctional Trypsin Protease Inhibitor from Yellow Bell Pepper Seeds: Uncovering Its Dual Antifungal and Hypoglycemic Properties

Fungal infections are a growing public health concern worldwide and the emergence of antifungal resistance has limited the number of therapeutic options. Therefore, developing novel strategies for identifying and developing new antifungal compounds is an active area of research in the pharmaceutical...

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Autores principales: Cotabarren, Juliana, Ozón, Brenda, Claver, Santiago, Geier, Florencia, Rossotti, Martina, Garcia-Pardo, Javier, Obregón, Walter David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10054557/
https://www.ncbi.nlm.nih.gov/pubmed/36986642
http://dx.doi.org/10.3390/pharmaceutics15030781
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author Cotabarren, Juliana
Ozón, Brenda
Claver, Santiago
Geier, Florencia
Rossotti, Martina
Garcia-Pardo, Javier
Obregón, Walter David
author_facet Cotabarren, Juliana
Ozón, Brenda
Claver, Santiago
Geier, Florencia
Rossotti, Martina
Garcia-Pardo, Javier
Obregón, Walter David
author_sort Cotabarren, Juliana
collection PubMed
description Fungal infections are a growing public health concern worldwide and the emergence of antifungal resistance has limited the number of therapeutic options. Therefore, developing novel strategies for identifying and developing new antifungal compounds is an active area of research in the pharmaceutical industry. In this study, we purified and characterized a trypsin protease inhibitor obtained from Yellow Bell Pepper (Capsicum annuum L.) seeds. The inhibitor not only showed potent and specific activity against the pathogenic fungus Candida albicans, but was also found to be non-toxic against human cells. Furthermore, this inhibitor is unique in that it also inhibits α-1,4-glucosidase, positioning it as one of the first plant-derived protease inhibitors with dual biological activity. This exciting discovery opens new avenues for the development of this inhibitor as a promising antifungal agent and highlights the potential of plant-derived protease inhibitors as a rich source for the discovery of novel multifunctional bioactive molecules.
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spelling pubmed-100545572023-03-30 A Multifunctional Trypsin Protease Inhibitor from Yellow Bell Pepper Seeds: Uncovering Its Dual Antifungal and Hypoglycemic Properties Cotabarren, Juliana Ozón, Brenda Claver, Santiago Geier, Florencia Rossotti, Martina Garcia-Pardo, Javier Obregón, Walter David Pharmaceutics Article Fungal infections are a growing public health concern worldwide and the emergence of antifungal resistance has limited the number of therapeutic options. Therefore, developing novel strategies for identifying and developing new antifungal compounds is an active area of research in the pharmaceutical industry. In this study, we purified and characterized a trypsin protease inhibitor obtained from Yellow Bell Pepper (Capsicum annuum L.) seeds. The inhibitor not only showed potent and specific activity against the pathogenic fungus Candida albicans, but was also found to be non-toxic against human cells. Furthermore, this inhibitor is unique in that it also inhibits α-1,4-glucosidase, positioning it as one of the first plant-derived protease inhibitors with dual biological activity. This exciting discovery opens new avenues for the development of this inhibitor as a promising antifungal agent and highlights the potential of plant-derived protease inhibitors as a rich source for the discovery of novel multifunctional bioactive molecules. MDPI 2023-02-27 /pmc/articles/PMC10054557/ /pubmed/36986642 http://dx.doi.org/10.3390/pharmaceutics15030781 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cotabarren, Juliana
Ozón, Brenda
Claver, Santiago
Geier, Florencia
Rossotti, Martina
Garcia-Pardo, Javier
Obregón, Walter David
A Multifunctional Trypsin Protease Inhibitor from Yellow Bell Pepper Seeds: Uncovering Its Dual Antifungal and Hypoglycemic Properties
title A Multifunctional Trypsin Protease Inhibitor from Yellow Bell Pepper Seeds: Uncovering Its Dual Antifungal and Hypoglycemic Properties
title_full A Multifunctional Trypsin Protease Inhibitor from Yellow Bell Pepper Seeds: Uncovering Its Dual Antifungal and Hypoglycemic Properties
title_fullStr A Multifunctional Trypsin Protease Inhibitor from Yellow Bell Pepper Seeds: Uncovering Its Dual Antifungal and Hypoglycemic Properties
title_full_unstemmed A Multifunctional Trypsin Protease Inhibitor from Yellow Bell Pepper Seeds: Uncovering Its Dual Antifungal and Hypoglycemic Properties
title_short A Multifunctional Trypsin Protease Inhibitor from Yellow Bell Pepper Seeds: Uncovering Its Dual Antifungal and Hypoglycemic Properties
title_sort multifunctional trypsin protease inhibitor from yellow bell pepper seeds: uncovering its dual antifungal and hypoglycemic properties
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10054557/
https://www.ncbi.nlm.nih.gov/pubmed/36986642
http://dx.doi.org/10.3390/pharmaceutics15030781
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