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A Single Nucleotide Variation of CRS2 Affected the Establishment of Photosynthetic System in Rice

Chloroplasts are essential sites for plant photosynthesis, and the biogenesis of the photosynthetic complexes involves the interaction of nuclear genes and chloroplast genes. In this study, we identified a rice pale green leaf mutant, crs2. The crs2 mutant showed different degrees of low chlorophyll...

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Autores principales: Chen, Hongwei, Wang, Qi, Fan, Mingqian, Zhang, Xijuan, Feng, Pulin, Zhu, Lin, Wu, Jiayi, Cheng, Xiaoyi, Wang, Jiayu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10054620/
https://www.ncbi.nlm.nih.gov/pubmed/36982870
http://dx.doi.org/10.3390/ijms24065796
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author Chen, Hongwei
Wang, Qi
Fan, Mingqian
Zhang, Xijuan
Feng, Pulin
Zhu, Lin
Wu, Jiayi
Cheng, Xiaoyi
Wang, Jiayu
author_facet Chen, Hongwei
Wang, Qi
Fan, Mingqian
Zhang, Xijuan
Feng, Pulin
Zhu, Lin
Wu, Jiayi
Cheng, Xiaoyi
Wang, Jiayu
author_sort Chen, Hongwei
collection PubMed
description Chloroplasts are essential sites for plant photosynthesis, and the biogenesis of the photosynthetic complexes involves the interaction of nuclear genes and chloroplast genes. In this study, we identified a rice pale green leaf mutant, crs2. The crs2 mutant showed different degrees of low chlorophyll phenotypes at different growth stages, especially at the seedling stage. Fine mapping and DNA sequencing of crs2 revealed a single nucleotide substitution (G4120A) in the eighth exons of CRS2, causing a G-to-R mutation of the 229th amino acid of CRS2 (G229R). The results of complementation experiments confirmed that this single-base mutation in crs2 is responsible for the phenotype of the crs2 mutant. CRS2 encodes a chloroplast RNA splicing 2 protein localized in the chloroplast. Western blot results revealed an abnormality in the abundance of the photosynthesis-related protein in crs2. However, the mutation of CRS2 leads to the enhancement of antioxidant enzyme activity, which could reduce ROS levels. Meanwhile, with the release of Rubisco activity, the photosynthetic performance of crs2 was improved. In summary, the G229R mutation in CRS2 causes chloroplast protein abnormalities and affects photosystem performance in rice; the above findings facilitate the elucidation of the physiological mechanism of chloroplast proteins affecting photosynthesis.
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spelling pubmed-100546202023-03-30 A Single Nucleotide Variation of CRS2 Affected the Establishment of Photosynthetic System in Rice Chen, Hongwei Wang, Qi Fan, Mingqian Zhang, Xijuan Feng, Pulin Zhu, Lin Wu, Jiayi Cheng, Xiaoyi Wang, Jiayu Int J Mol Sci Article Chloroplasts are essential sites for plant photosynthesis, and the biogenesis of the photosynthetic complexes involves the interaction of nuclear genes and chloroplast genes. In this study, we identified a rice pale green leaf mutant, crs2. The crs2 mutant showed different degrees of low chlorophyll phenotypes at different growth stages, especially at the seedling stage. Fine mapping and DNA sequencing of crs2 revealed a single nucleotide substitution (G4120A) in the eighth exons of CRS2, causing a G-to-R mutation of the 229th amino acid of CRS2 (G229R). The results of complementation experiments confirmed that this single-base mutation in crs2 is responsible for the phenotype of the crs2 mutant. CRS2 encodes a chloroplast RNA splicing 2 protein localized in the chloroplast. Western blot results revealed an abnormality in the abundance of the photosynthesis-related protein in crs2. However, the mutation of CRS2 leads to the enhancement of antioxidant enzyme activity, which could reduce ROS levels. Meanwhile, with the release of Rubisco activity, the photosynthetic performance of crs2 was improved. In summary, the G229R mutation in CRS2 causes chloroplast protein abnormalities and affects photosystem performance in rice; the above findings facilitate the elucidation of the physiological mechanism of chloroplast proteins affecting photosynthesis. MDPI 2023-03-18 /pmc/articles/PMC10054620/ /pubmed/36982870 http://dx.doi.org/10.3390/ijms24065796 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Hongwei
Wang, Qi
Fan, Mingqian
Zhang, Xijuan
Feng, Pulin
Zhu, Lin
Wu, Jiayi
Cheng, Xiaoyi
Wang, Jiayu
A Single Nucleotide Variation of CRS2 Affected the Establishment of Photosynthetic System in Rice
title A Single Nucleotide Variation of CRS2 Affected the Establishment of Photosynthetic System in Rice
title_full A Single Nucleotide Variation of CRS2 Affected the Establishment of Photosynthetic System in Rice
title_fullStr A Single Nucleotide Variation of CRS2 Affected the Establishment of Photosynthetic System in Rice
title_full_unstemmed A Single Nucleotide Variation of CRS2 Affected the Establishment of Photosynthetic System in Rice
title_short A Single Nucleotide Variation of CRS2 Affected the Establishment of Photosynthetic System in Rice
title_sort single nucleotide variation of crs2 affected the establishment of photosynthetic system in rice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10054620/
https://www.ncbi.nlm.nih.gov/pubmed/36982870
http://dx.doi.org/10.3390/ijms24065796
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