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Chemical Constituents of Thesium chinense Turcz and Their In Vitro Antioxidant, Anti-Inflammatory and Cytotoxic Activities

Three novel compounds (1–3) along with twenty-six known compounds, two known steroids (4–5) and twenty-four known phenylpropanoids (6–29) were isolated from the whole plant of Thesium chinense Turcz. The structures of the three new compounds were elucidated on the basis of ESI-MS, HR-ESIMS, 1D and 2...

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Autores principales: Liu, Zhen-Zhen, Ma, Jun-Cheng, Deng, Peng, Ren, Fu-Cai, Li, Ning
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10054634/
https://www.ncbi.nlm.nih.gov/pubmed/36985657
http://dx.doi.org/10.3390/molecules28062685
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author Liu, Zhen-Zhen
Ma, Jun-Cheng
Deng, Peng
Ren, Fu-Cai
Li, Ning
author_facet Liu, Zhen-Zhen
Ma, Jun-Cheng
Deng, Peng
Ren, Fu-Cai
Li, Ning
author_sort Liu, Zhen-Zhen
collection PubMed
description Three novel compounds (1–3) along with twenty-six known compounds, two known steroids (4–5) and twenty-four known phenylpropanoids (6–29) were isolated from the whole plant of Thesium chinense Turcz. The structures of the three new compounds were elucidated on the basis of ESI-MS, HR-ESIMS, 1D and 2D NMR, IR, UV spectroscopic data. The absolute stereochemistry of compound 1 was determined by the Gauge-Including Atomic Orbitals (GIAO) method. The in vitro antioxidant, anti-inflammatory and cytotoxic activities of the isolated compounds were evaluated by DPPH radical-scavenging assay, LPS-activated RAW 264.7 cells model and CCK-8 kit, respectively. Compound 11 showed high antioxidant activity with an SC(50) value of 16.2 ± 1.6 μM. Compound 21 showed considerable anti-inflammatory activity with an IC(50) value of 28.6 ± 3.0 μM. Compounds 4 and 5 displayed potent cytotoxic activity against human NCI-H292, SiHa, A549, and MKN45 cell lines, with the compound 4 having IC(50) values of 17.4 ± 2.4, 22.2 ± 1.1, 9.7 ± 0.9, 9.5 ±0.7 μM, and the compound 5 having all IC(50) values less than 0.1 μM in vitro.
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spelling pubmed-100546342023-03-30 Chemical Constituents of Thesium chinense Turcz and Their In Vitro Antioxidant, Anti-Inflammatory and Cytotoxic Activities Liu, Zhen-Zhen Ma, Jun-Cheng Deng, Peng Ren, Fu-Cai Li, Ning Molecules Article Three novel compounds (1–3) along with twenty-six known compounds, two known steroids (4–5) and twenty-four known phenylpropanoids (6–29) were isolated from the whole plant of Thesium chinense Turcz. The structures of the three new compounds were elucidated on the basis of ESI-MS, HR-ESIMS, 1D and 2D NMR, IR, UV spectroscopic data. The absolute stereochemistry of compound 1 was determined by the Gauge-Including Atomic Orbitals (GIAO) method. The in vitro antioxidant, anti-inflammatory and cytotoxic activities of the isolated compounds were evaluated by DPPH radical-scavenging assay, LPS-activated RAW 264.7 cells model and CCK-8 kit, respectively. Compound 11 showed high antioxidant activity with an SC(50) value of 16.2 ± 1.6 μM. Compound 21 showed considerable anti-inflammatory activity with an IC(50) value of 28.6 ± 3.0 μM. Compounds 4 and 5 displayed potent cytotoxic activity against human NCI-H292, SiHa, A549, and MKN45 cell lines, with the compound 4 having IC(50) values of 17.4 ± 2.4, 22.2 ± 1.1, 9.7 ± 0.9, 9.5 ±0.7 μM, and the compound 5 having all IC(50) values less than 0.1 μM in vitro. MDPI 2023-03-16 /pmc/articles/PMC10054634/ /pubmed/36985657 http://dx.doi.org/10.3390/molecules28062685 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Zhen-Zhen
Ma, Jun-Cheng
Deng, Peng
Ren, Fu-Cai
Li, Ning
Chemical Constituents of Thesium chinense Turcz and Their In Vitro Antioxidant, Anti-Inflammatory and Cytotoxic Activities
title Chemical Constituents of Thesium chinense Turcz and Their In Vitro Antioxidant, Anti-Inflammatory and Cytotoxic Activities
title_full Chemical Constituents of Thesium chinense Turcz and Their In Vitro Antioxidant, Anti-Inflammatory and Cytotoxic Activities
title_fullStr Chemical Constituents of Thesium chinense Turcz and Their In Vitro Antioxidant, Anti-Inflammatory and Cytotoxic Activities
title_full_unstemmed Chemical Constituents of Thesium chinense Turcz and Their In Vitro Antioxidant, Anti-Inflammatory and Cytotoxic Activities
title_short Chemical Constituents of Thesium chinense Turcz and Their In Vitro Antioxidant, Anti-Inflammatory and Cytotoxic Activities
title_sort chemical constituents of thesium chinense turcz and their in vitro antioxidant, anti-inflammatory and cytotoxic activities
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10054634/
https://www.ncbi.nlm.nih.gov/pubmed/36985657
http://dx.doi.org/10.3390/molecules28062685
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