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Approaches for Modes of Action Study of Long Non-Coding RNAs: From Single Verification to Genome-Wide Determination
Long non-coding RNAs (lncRNAs) are transcripts longer than 200 nucleotides (nt) that are not translated into known functional proteins. This broad definition covers a large collection of transcripts with diverse genomic origins, biogenesis, and modes of action. Thus, it is very important to choose a...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10054671/ https://www.ncbi.nlm.nih.gov/pubmed/36982636 http://dx.doi.org/10.3390/ijms24065562 |
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author | Tao, Xiaoyuan Li, Sujuan Chen, Guang Wang, Jian Xu, Shengchun |
author_facet | Tao, Xiaoyuan Li, Sujuan Chen, Guang Wang, Jian Xu, Shengchun |
author_sort | Tao, Xiaoyuan |
collection | PubMed |
description | Long non-coding RNAs (lncRNAs) are transcripts longer than 200 nucleotides (nt) that are not translated into known functional proteins. This broad definition covers a large collection of transcripts with diverse genomic origins, biogenesis, and modes of action. Thus, it is very important to choose appropriate research methodologies when investigating lncRNAs with biological significance. Multiple reviews to date have summarized the mechanisms of lncRNA biogenesis, their localization, their functions in gene regulation at multiple levels, and also their potential applications. However, little has been reviewed on the leading strategies for lncRNA research. Here, we generalize a basic and systemic mind map for lncRNA research and discuss the mechanisms and the application scenarios of ‘up-to-date’ techniques as applied to molecular function studies of lncRNAs. Taking advantage of documented lncRNA research paradigms as examples, we aim to provide an overview of the developing techniques for elucidating lncRNA interactions with genomic DNA, proteins, and other RNAs. In the end, we propose the future direction and potential technological challenges of lncRNA studies, focusing on techniques and applications. |
format | Online Article Text |
id | pubmed-10054671 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100546712023-03-30 Approaches for Modes of Action Study of Long Non-Coding RNAs: From Single Verification to Genome-Wide Determination Tao, Xiaoyuan Li, Sujuan Chen, Guang Wang, Jian Xu, Shengchun Int J Mol Sci Review Long non-coding RNAs (lncRNAs) are transcripts longer than 200 nucleotides (nt) that are not translated into known functional proteins. This broad definition covers a large collection of transcripts with diverse genomic origins, biogenesis, and modes of action. Thus, it is very important to choose appropriate research methodologies when investigating lncRNAs with biological significance. Multiple reviews to date have summarized the mechanisms of lncRNA biogenesis, their localization, their functions in gene regulation at multiple levels, and also their potential applications. However, little has been reviewed on the leading strategies for lncRNA research. Here, we generalize a basic and systemic mind map for lncRNA research and discuss the mechanisms and the application scenarios of ‘up-to-date’ techniques as applied to molecular function studies of lncRNAs. Taking advantage of documented lncRNA research paradigms as examples, we aim to provide an overview of the developing techniques for elucidating lncRNA interactions with genomic DNA, proteins, and other RNAs. In the end, we propose the future direction and potential technological challenges of lncRNA studies, focusing on techniques and applications. MDPI 2023-03-14 /pmc/articles/PMC10054671/ /pubmed/36982636 http://dx.doi.org/10.3390/ijms24065562 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Tao, Xiaoyuan Li, Sujuan Chen, Guang Wang, Jian Xu, Shengchun Approaches for Modes of Action Study of Long Non-Coding RNAs: From Single Verification to Genome-Wide Determination |
title | Approaches for Modes of Action Study of Long Non-Coding RNAs: From Single Verification to Genome-Wide Determination |
title_full | Approaches for Modes of Action Study of Long Non-Coding RNAs: From Single Verification to Genome-Wide Determination |
title_fullStr | Approaches for Modes of Action Study of Long Non-Coding RNAs: From Single Verification to Genome-Wide Determination |
title_full_unstemmed | Approaches for Modes of Action Study of Long Non-Coding RNAs: From Single Verification to Genome-Wide Determination |
title_short | Approaches for Modes of Action Study of Long Non-Coding RNAs: From Single Verification to Genome-Wide Determination |
title_sort | approaches for modes of action study of long non-coding rnas: from single verification to genome-wide determination |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10054671/ https://www.ncbi.nlm.nih.gov/pubmed/36982636 http://dx.doi.org/10.3390/ijms24065562 |
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