Cargando…
Rapid Induction of Protective Immunity against Pneumonic Plague by Yersinia pestis Polymeric F1 and LcrV Antigens
In a recent study, we demonstrated that vaccination with the polymeric F1 capsule antigen of the plague pathogen Yersinia pestis led to the rapid induction of a protective humoral immune response via the pivotal activation of innate-like B1b cells. Conversely, the monomeric version of F1 failed to p...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10054699/ https://www.ncbi.nlm.nih.gov/pubmed/36992165 http://dx.doi.org/10.3390/vaccines11030581 |
_version_ | 1785015733929902080 |
---|---|
author | Aftalion, Moshe Tidhar, Avital Vagima, Yaron Gur, David Zauberman, Ayelet Holtzman, Tzvi Makovitzki, Arik Chitlaru, Theodor Mamroud, Emanuelle Levy, Yinon |
author_facet | Aftalion, Moshe Tidhar, Avital Vagima, Yaron Gur, David Zauberman, Ayelet Holtzman, Tzvi Makovitzki, Arik Chitlaru, Theodor Mamroud, Emanuelle Levy, Yinon |
author_sort | Aftalion, Moshe |
collection | PubMed |
description | In a recent study, we demonstrated that vaccination with the polymeric F1 capsule antigen of the plague pathogen Yersinia pestis led to the rapid induction of a protective humoral immune response via the pivotal activation of innate-like B1b cells. Conversely, the monomeric version of F1 failed to promptly protect vaccinated animals in this model of the bubonic plague. In this study, we examined the ability of F1 to confer the rapid onset of protective immunity in the more challenging mouse model of the pneumonic plague. Vaccination with one dose of F1 adsorbed on aluminum hydroxide elicited effective protection against subsequent lethal intranasal exposure to a fully virulent Y. pestis strain within a week. Interestingly, the addition of the LcrV antigen shortened the time required for achieving such rapid protective immunity to 4–5 days after vaccination. As found previously, the polymeric structure of F1 was essential in affording the accelerated protective response observed by covaccination with LcrV. Finally, in a longevity study, a single vaccination with polymeric F1 induced a higher and more uniform humoral response than a similar vaccination with monomeric F1. However, in this setting, the dominant contribution of LcrV to long-lasting immunity against a lethal pulmonary challenge was reiterated. |
format | Online Article Text |
id | pubmed-10054699 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100546992023-03-30 Rapid Induction of Protective Immunity against Pneumonic Plague by Yersinia pestis Polymeric F1 and LcrV Antigens Aftalion, Moshe Tidhar, Avital Vagima, Yaron Gur, David Zauberman, Ayelet Holtzman, Tzvi Makovitzki, Arik Chitlaru, Theodor Mamroud, Emanuelle Levy, Yinon Vaccines (Basel) Article In a recent study, we demonstrated that vaccination with the polymeric F1 capsule antigen of the plague pathogen Yersinia pestis led to the rapid induction of a protective humoral immune response via the pivotal activation of innate-like B1b cells. Conversely, the monomeric version of F1 failed to promptly protect vaccinated animals in this model of the bubonic plague. In this study, we examined the ability of F1 to confer the rapid onset of protective immunity in the more challenging mouse model of the pneumonic plague. Vaccination with one dose of F1 adsorbed on aluminum hydroxide elicited effective protection against subsequent lethal intranasal exposure to a fully virulent Y. pestis strain within a week. Interestingly, the addition of the LcrV antigen shortened the time required for achieving such rapid protective immunity to 4–5 days after vaccination. As found previously, the polymeric structure of F1 was essential in affording the accelerated protective response observed by covaccination with LcrV. Finally, in a longevity study, a single vaccination with polymeric F1 induced a higher and more uniform humoral response than a similar vaccination with monomeric F1. However, in this setting, the dominant contribution of LcrV to long-lasting immunity against a lethal pulmonary challenge was reiterated. MDPI 2023-03-02 /pmc/articles/PMC10054699/ /pubmed/36992165 http://dx.doi.org/10.3390/vaccines11030581 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Aftalion, Moshe Tidhar, Avital Vagima, Yaron Gur, David Zauberman, Ayelet Holtzman, Tzvi Makovitzki, Arik Chitlaru, Theodor Mamroud, Emanuelle Levy, Yinon Rapid Induction of Protective Immunity against Pneumonic Plague by Yersinia pestis Polymeric F1 and LcrV Antigens |
title | Rapid Induction of Protective Immunity against Pneumonic Plague by Yersinia pestis Polymeric F1 and LcrV Antigens |
title_full | Rapid Induction of Protective Immunity against Pneumonic Plague by Yersinia pestis Polymeric F1 and LcrV Antigens |
title_fullStr | Rapid Induction of Protective Immunity against Pneumonic Plague by Yersinia pestis Polymeric F1 and LcrV Antigens |
title_full_unstemmed | Rapid Induction of Protective Immunity against Pneumonic Plague by Yersinia pestis Polymeric F1 and LcrV Antigens |
title_short | Rapid Induction of Protective Immunity against Pneumonic Plague by Yersinia pestis Polymeric F1 and LcrV Antigens |
title_sort | rapid induction of protective immunity against pneumonic plague by yersinia pestis polymeric f1 and lcrv antigens |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10054699/ https://www.ncbi.nlm.nih.gov/pubmed/36992165 http://dx.doi.org/10.3390/vaccines11030581 |
work_keys_str_mv | AT aftalionmoshe rapidinductionofprotectiveimmunityagainstpneumonicplaguebyyersiniapestispolymericf1andlcrvantigens AT tidharavital rapidinductionofprotectiveimmunityagainstpneumonicplaguebyyersiniapestispolymericf1andlcrvantigens AT vagimayaron rapidinductionofprotectiveimmunityagainstpneumonicplaguebyyersiniapestispolymericf1andlcrvantigens AT gurdavid rapidinductionofprotectiveimmunityagainstpneumonicplaguebyyersiniapestispolymericf1andlcrvantigens AT zaubermanayelet rapidinductionofprotectiveimmunityagainstpneumonicplaguebyyersiniapestispolymericf1andlcrvantigens AT holtzmantzvi rapidinductionofprotectiveimmunityagainstpneumonicplaguebyyersiniapestispolymericf1andlcrvantigens AT makovitzkiarik rapidinductionofprotectiveimmunityagainstpneumonicplaguebyyersiniapestispolymericf1andlcrvantigens AT chitlarutheodor rapidinductionofprotectiveimmunityagainstpneumonicplaguebyyersiniapestispolymericf1andlcrvantigens AT mamroudemanuelle rapidinductionofprotectiveimmunityagainstpneumonicplaguebyyersiniapestispolymericf1andlcrvantigens AT levyyinon rapidinductionofprotectiveimmunityagainstpneumonicplaguebyyersiniapestispolymericf1andlcrvantigens |