Cargando…

Rapid Induction of Protective Immunity against Pneumonic Plague by Yersinia pestis Polymeric F1 and LcrV Antigens

In a recent study, we demonstrated that vaccination with the polymeric F1 capsule antigen of the plague pathogen Yersinia pestis led to the rapid induction of a protective humoral immune response via the pivotal activation of innate-like B1b cells. Conversely, the monomeric version of F1 failed to p...

Descripción completa

Detalles Bibliográficos
Autores principales: Aftalion, Moshe, Tidhar, Avital, Vagima, Yaron, Gur, David, Zauberman, Ayelet, Holtzman, Tzvi, Makovitzki, Arik, Chitlaru, Theodor, Mamroud, Emanuelle, Levy, Yinon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10054699/
https://www.ncbi.nlm.nih.gov/pubmed/36992165
http://dx.doi.org/10.3390/vaccines11030581
_version_ 1785015733929902080
author Aftalion, Moshe
Tidhar, Avital
Vagima, Yaron
Gur, David
Zauberman, Ayelet
Holtzman, Tzvi
Makovitzki, Arik
Chitlaru, Theodor
Mamroud, Emanuelle
Levy, Yinon
author_facet Aftalion, Moshe
Tidhar, Avital
Vagima, Yaron
Gur, David
Zauberman, Ayelet
Holtzman, Tzvi
Makovitzki, Arik
Chitlaru, Theodor
Mamroud, Emanuelle
Levy, Yinon
author_sort Aftalion, Moshe
collection PubMed
description In a recent study, we demonstrated that vaccination with the polymeric F1 capsule antigen of the plague pathogen Yersinia pestis led to the rapid induction of a protective humoral immune response via the pivotal activation of innate-like B1b cells. Conversely, the monomeric version of F1 failed to promptly protect vaccinated animals in this model of the bubonic plague. In this study, we examined the ability of F1 to confer the rapid onset of protective immunity in the more challenging mouse model of the pneumonic plague. Vaccination with one dose of F1 adsorbed on aluminum hydroxide elicited effective protection against subsequent lethal intranasal exposure to a fully virulent Y. pestis strain within a week. Interestingly, the addition of the LcrV antigen shortened the time required for achieving such rapid protective immunity to 4–5 days after vaccination. As found previously, the polymeric structure of F1 was essential in affording the accelerated protective response observed by covaccination with LcrV. Finally, in a longevity study, a single vaccination with polymeric F1 induced a higher and more uniform humoral response than a similar vaccination with monomeric F1. However, in this setting, the dominant contribution of LcrV to long-lasting immunity against a lethal pulmonary challenge was reiterated.
format Online
Article
Text
id pubmed-10054699
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-100546992023-03-30 Rapid Induction of Protective Immunity against Pneumonic Plague by Yersinia pestis Polymeric F1 and LcrV Antigens Aftalion, Moshe Tidhar, Avital Vagima, Yaron Gur, David Zauberman, Ayelet Holtzman, Tzvi Makovitzki, Arik Chitlaru, Theodor Mamroud, Emanuelle Levy, Yinon Vaccines (Basel) Article In a recent study, we demonstrated that vaccination with the polymeric F1 capsule antigen of the plague pathogen Yersinia pestis led to the rapid induction of a protective humoral immune response via the pivotal activation of innate-like B1b cells. Conversely, the monomeric version of F1 failed to promptly protect vaccinated animals in this model of the bubonic plague. In this study, we examined the ability of F1 to confer the rapid onset of protective immunity in the more challenging mouse model of the pneumonic plague. Vaccination with one dose of F1 adsorbed on aluminum hydroxide elicited effective protection against subsequent lethal intranasal exposure to a fully virulent Y. pestis strain within a week. Interestingly, the addition of the LcrV antigen shortened the time required for achieving such rapid protective immunity to 4–5 days after vaccination. As found previously, the polymeric structure of F1 was essential in affording the accelerated protective response observed by covaccination with LcrV. Finally, in a longevity study, a single vaccination with polymeric F1 induced a higher and more uniform humoral response than a similar vaccination with monomeric F1. However, in this setting, the dominant contribution of LcrV to long-lasting immunity against a lethal pulmonary challenge was reiterated. MDPI 2023-03-02 /pmc/articles/PMC10054699/ /pubmed/36992165 http://dx.doi.org/10.3390/vaccines11030581 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Aftalion, Moshe
Tidhar, Avital
Vagima, Yaron
Gur, David
Zauberman, Ayelet
Holtzman, Tzvi
Makovitzki, Arik
Chitlaru, Theodor
Mamroud, Emanuelle
Levy, Yinon
Rapid Induction of Protective Immunity against Pneumonic Plague by Yersinia pestis Polymeric F1 and LcrV Antigens
title Rapid Induction of Protective Immunity against Pneumonic Plague by Yersinia pestis Polymeric F1 and LcrV Antigens
title_full Rapid Induction of Protective Immunity against Pneumonic Plague by Yersinia pestis Polymeric F1 and LcrV Antigens
title_fullStr Rapid Induction of Protective Immunity against Pneumonic Plague by Yersinia pestis Polymeric F1 and LcrV Antigens
title_full_unstemmed Rapid Induction of Protective Immunity against Pneumonic Plague by Yersinia pestis Polymeric F1 and LcrV Antigens
title_short Rapid Induction of Protective Immunity against Pneumonic Plague by Yersinia pestis Polymeric F1 and LcrV Antigens
title_sort rapid induction of protective immunity against pneumonic plague by yersinia pestis polymeric f1 and lcrv antigens
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10054699/
https://www.ncbi.nlm.nih.gov/pubmed/36992165
http://dx.doi.org/10.3390/vaccines11030581
work_keys_str_mv AT aftalionmoshe rapidinductionofprotectiveimmunityagainstpneumonicplaguebyyersiniapestispolymericf1andlcrvantigens
AT tidharavital rapidinductionofprotectiveimmunityagainstpneumonicplaguebyyersiniapestispolymericf1andlcrvantigens
AT vagimayaron rapidinductionofprotectiveimmunityagainstpneumonicplaguebyyersiniapestispolymericf1andlcrvantigens
AT gurdavid rapidinductionofprotectiveimmunityagainstpneumonicplaguebyyersiniapestispolymericf1andlcrvantigens
AT zaubermanayelet rapidinductionofprotectiveimmunityagainstpneumonicplaguebyyersiniapestispolymericf1andlcrvantigens
AT holtzmantzvi rapidinductionofprotectiveimmunityagainstpneumonicplaguebyyersiniapestispolymericf1andlcrvantigens
AT makovitzkiarik rapidinductionofprotectiveimmunityagainstpneumonicplaguebyyersiniapestispolymericf1andlcrvantigens
AT chitlarutheodor rapidinductionofprotectiveimmunityagainstpneumonicplaguebyyersiniapestispolymericf1andlcrvantigens
AT mamroudemanuelle rapidinductionofprotectiveimmunityagainstpneumonicplaguebyyersiniapestispolymericf1andlcrvantigens
AT levyyinon rapidinductionofprotectiveimmunityagainstpneumonicplaguebyyersiniapestispolymericf1andlcrvantigens