Cargando…

A modular cloning (MoClo) toolkit for reliable intracellular protein targeting in the yeast Saccharomyces cerevisiae

Modular Cloning (MoClo) allows the combinatorial assembly of plasmids from standardized genetic parts without the need of error-prone PCR reactions. It is a very powerful strategy which enables highly flexible expression patterns without the need of repetitive cloning procedures. In this study, we d...

Descripción completa

Detalles Bibliográficos
Autores principales: Simakin, Pavel, Koch, Christian, Herrmann, Johannes M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Shared Science Publishers OG 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10054711/
https://www.ncbi.nlm.nih.gov/pubmed/37009624
http://dx.doi.org/10.15698/mic2023.04.794