Cargando…

Using DNA flow-stretching assay as a tool to validate the tagging of DNA-binding proteins for single-molecule experiments

Due to the enhanced labeling capability of maleimide-based fluorescent probes, lysine-cysteine-lysine (KCK) tags are frequently added to proteins for visualization. In this study, we employed in vitro single-molecule DNA flow-stretching assay as a sensitive way to assess the impact of the KCK-tag on...

Descripción completa

Detalles Bibliográficos
Autores principales:	Molina, Miranda, Way, Lindsey E., Ren, Zhongqing, Liao, Qin, Wang, Xindan, Kim, HyeongJun
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Cold Spring Harbor Laboratory 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10055205/ https://www.ncbi.nlm.nih.gov/pubmed/36993356 http://dx.doi.org/10.1101/2023.03.19.533373

Ejemplares similares

A framework to validate fluorescently labeled DNA-binding proteins for single-molecule experiments
por: Molina, Miranda, et al.
Publicado: (2023)

Multiplexed single-molecule flow-stretching bead assay for DNA enzymology
por: Lee, Ryanggeun, et al.
Publicado: (2019)

A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA
por: Xiong, Kan, et al.
Publicado: (2017)

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay
por: Kulczyk, Arkadiusz W., et al.
Publicado: (2010)

Multistep assembly of DNA condensation clusters by SMC
por: Kim, HyeongJun, et al.
Publicado: (2016)

Quantifying the stability of oxidatively damaged DNA by single-molecule DNA stretching
por: McCauley, Micah J, et al.
Publicado: (2018)

A simple DNA stretching method for fluorescence imaging of single DNA molecules
por: Chan, Ting-Fung, et al.
Publicado: (2006)

Stretching and immobilization of DNA for studies of protein–DNA interactions at the single-molecule level
por: Kim, JiHoon, et al.
Publicado: (2007)

A Colorimetric Microplate Assay for DNA-Binding Activity of His-Tagged MutS Protein
por: Banasik, Michał, et al.
Publicado: (2016)

Application of single molecule technology to rapidly map long DNA and study the conformation of stretched DNA
por: Phillips, Kevin M., et al.
Publicado: (2005)

Sequencing of long stretches of repetitive DNA
por: De Bustos, Alfredo, et al.
Publicado: (2016)

Accurate gene synthesis with tag-directed retrieval of sequence-verified DNA molecules
por: Schwartz, Jerrod J, et al.
Publicado: (2012)

Single-molecule analysis of 1D diffusion and transcription elongation of T7 RNA polymerase along individual stretched DNA molecules
por: Kim, Ji Hoon, et al.
Publicado: (2007)

Z-DNA as a Tool for Nuclease-Free DNA Methyltransferase Assay
por: Kim, Sook Ho, et al.
Publicado: (2021)

Molecular Threading: Mechanical Extraction, Stretching and Placement of DNA Molecules from a Liquid-Air Interface
por: Payne, Andrew C., et al.
Publicado: (2013)

DNA molecule stretching through thermo-electrophoresis and thermal convection in a heated converging-diverging microchannel
por: Hsieh, Shou-Shing, et al.
Publicado: (2013)

Design of Sequence-Specific DNA Binding Molecules for DNA Methyltransferase Inhibition
por: Kang, JeenJoo S., et al.
Publicado: (2014)

DNA binding fluorescent proteins for the direct visualization of large DNA molecules
por: Lee, Seonghyun, et al.
Publicado: (2016)

DNA Origami Seesaws as Comparative Binding Assay
por: Nickels, Philipp C., et al.
Publicado: (2016)

Universality in RNA and DNA deformations induced by salt, temperature change, stretching force, and protein binding
por: Tian, Fu-Jia, et al.
Publicado: (2023)

Modulating the G‐Quadruplex and Duplex DNA Binding by Controlling the Charge of Fluorescent Molecules
por: Gil‐Martínez, Ariadna, et al.
Publicado: (2022)

Multiplexed Microsphere Suspension-Array Assay for Urine Mitochondrial DNA Typing by C-Stretch Length in Hypervariable Regions
por: Aoki, Kimiko, et al.
Publicado: (2018)

A versatile non-radioactive assay for DNA methyltransferase activity and DNA binding
por: Frauer, Carina, et al.
Publicado: (2009)

Universal strategies for the DNA-encoding of libraries of small molecules using the chemical ligation of oligonucleotide tags
por: Litovchick, Alexander, et al.
Publicado: (2014)

Terminal Protection of Small Molecule-Linked DNA for Small Molecule–Protein Interaction Assays
por: Hu, Cui, et al.
Publicado: (2014)

Amplified stretch of bottlebrush-coated DNA in nanofluidic channels
por: Zhang, Ce, et al.
Publicado: (2013)

Stick, stretch, and scan imaging method for DNA and filaments
por: Zeng, Qiuling, et al.
Publicado: (2021)

Direct optical mapping of transcription factor binding sites on field-stretched λ-DNA in nanofluidic devices
por: Sriram, K. K., et al.
Publicado: (2014)

CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins
por: Savic, Daniel, et al.
Publicado: (2015)

A Horizontal Magnetic Tweezers for Studying Single DNA Molecules and DNA-Binding Proteins
por: Fabian, Roberto, et al.
Publicado: (2021)

DNA-binding proteins studied by mechanical manipulation and AFM imaging of single DNA molecules
por: Zhao, Xiaodan, et al.
Publicado: (2022)

Rabi oscillations in a stretching molecule
por: Pan, Shengzhe, et al.
Publicado: (2023)

Strategies for Controlling the Spatial Orientation of Single Molecules Tethered on DNA Origami Templates Physisorbed on Glass Substrates: Intercalation and Stretching
por: Cervantes-Salguero, Keitel, et al.
Publicado: (2022)

TagGD: Fast and Accurate Software for DNA Tag Generation and Demultiplexing
por: Costea, Paul Igor, et al.
Publicado: (2013)

Sequence-based prediction of permissive stretches for internal protein tagging and knockdown
por: Oesterle, Sabine, et al.
Publicado: (2017)

DNA Y Structure: A Versatile, Multidimensional Single Molecule Assay
por: Inman, James T., et al.
Publicado: (2014)

DNA stretching by bacterial initiators promotes replication origin melting
por: Duderstadt, Karl E., et al.
Publicado: (2011)

DNA stretching in the nucleosome facilitates alkylation by an intercalating antitumour agent
por: Davey, Gabriela E., et al.
Publicado: (2010)

DNA stretching on the wall surfaces in curved microchannels with different radii
por: Hsieh, Shou-Shing, et al.
Publicado: (2014)

Intrinsic Stepwise Translocation of Stretched ssDNA in Graphene Nanopores
por: Qiu, Hu, et al.
Publicado: (2015)

Cannot write session to /tmp/vufind_sessions/sess_pu2d8s2d237o5gjp3ib5tcvprc