A simple method to dramatically increase C. elegans germline microinjection efficiency

Genome manipulation methods in C. elegans require microinjecting DNA or ribonucleoprotein complexes into the microscopic core of the gonadal syncytium. These microinjections are technically demanding and represent a key bottleneck for all genome engineering and transgenic approaches in C. elegans. W...

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Detalles Bibliográficos
Autores principales: Gibney, Theresa V., Favichia, Michelle, Latifi, Laila, Medwig-Kinney, Taylor N., Matus, David Q., McIntyre, Daniel C., Arrigo, Angelo B., Branham, Kendall R., Bubrig, Louis T., Ghaddar, Abbas, Jiranek, Juliana A., Liu, Kendra E., Marcucci, Charles G., Porter, Robert J., Pani, Ariel M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10055348/
https://www.ncbi.nlm.nih.gov/pubmed/36993165
http://dx.doi.org/10.1101/2023.03.23.533855

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