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Molecular heterogeneity of C. elegans glia across sexes
A comprehensive description of nervous system function, and sex dimorphism within, is incomplete without clear assessment of the diversity of its component cell types, neurons and glia. C. elegans has an invariant nervous system with the first mapped connectome of a multicellular organism and single...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10055349/ https://www.ncbi.nlm.nih.gov/pubmed/36993469 http://dx.doi.org/10.1101/2023.03.21.533668 |
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author | Purice, Maria D. Quitevis, Elgene J.A. Manning, R. Sean Severs, Liza J. Tran, Nina-Tuyen Sorrentino, Violet Setty, Manu Singhvi, Aakanksha |
author_facet | Purice, Maria D. Quitevis, Elgene J.A. Manning, R. Sean Severs, Liza J. Tran, Nina-Tuyen Sorrentino, Violet Setty, Manu Singhvi, Aakanksha |
author_sort | Purice, Maria D. |
collection | PubMed |
description | A comprehensive description of nervous system function, and sex dimorphism within, is incomplete without clear assessment of the diversity of its component cell types, neurons and glia. C. elegans has an invariant nervous system with the first mapped connectome of a multicellular organism and single-cell atlas of component neurons. Here we present single nuclear RNA-seq evaluation of glia across the entire adult C. elegans nervous system, including both sexes. Machine learning models enabled us to identify both sex-shared and sex-specific glia and glial subclasses. We have identified and validated molecular markers in silico and in vivo for these molecular subcategories. Comparative analytics also reveals previously unappreciated molecular heterogeneity in anatomically identical glia between and within sexes, indicating consequent functional heterogeneity. Furthermore, our datasets reveal that while adult C. elegans glia express neuropeptide genes, they lack the canonical unc-31/CAPS-dependent dense core vesicle release machinery. Thus, glia employ alternate neuromodulator processing mechanisms. Overall, this molecular atlas, available at www.wormglia.org, reveals rich insights into heterogeneity and sex dimorphism in glia across the entire nervous system of an adult animal. |
format | Online Article Text |
id | pubmed-10055349 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Cold Spring Harbor Laboratory |
record_format | MEDLINE/PubMed |
spelling | pubmed-100553492023-03-30 Molecular heterogeneity of C. elegans glia across sexes Purice, Maria D. Quitevis, Elgene J.A. Manning, R. Sean Severs, Liza J. Tran, Nina-Tuyen Sorrentino, Violet Setty, Manu Singhvi, Aakanksha bioRxiv Article A comprehensive description of nervous system function, and sex dimorphism within, is incomplete without clear assessment of the diversity of its component cell types, neurons and glia. C. elegans has an invariant nervous system with the first mapped connectome of a multicellular organism and single-cell atlas of component neurons. Here we present single nuclear RNA-seq evaluation of glia across the entire adult C. elegans nervous system, including both sexes. Machine learning models enabled us to identify both sex-shared and sex-specific glia and glial subclasses. We have identified and validated molecular markers in silico and in vivo for these molecular subcategories. Comparative analytics also reveals previously unappreciated molecular heterogeneity in anatomically identical glia between and within sexes, indicating consequent functional heterogeneity. Furthermore, our datasets reveal that while adult C. elegans glia express neuropeptide genes, they lack the canonical unc-31/CAPS-dependent dense core vesicle release machinery. Thus, glia employ alternate neuromodulator processing mechanisms. Overall, this molecular atlas, available at www.wormglia.org, reveals rich insights into heterogeneity and sex dimorphism in glia across the entire nervous system of an adult animal. Cold Spring Harbor Laboratory 2023-03-24 /pmc/articles/PMC10055349/ /pubmed/36993469 http://dx.doi.org/10.1101/2023.03.21.533668 Text en https://creativecommons.org/licenses/by-nc/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (https://creativecommons.org/licenses/by-nc/4.0/) , which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format for noncommercial purposes only, and only so long as attribution is given to the creator. |
spellingShingle | Article Purice, Maria D. Quitevis, Elgene J.A. Manning, R. Sean Severs, Liza J. Tran, Nina-Tuyen Sorrentino, Violet Setty, Manu Singhvi, Aakanksha Molecular heterogeneity of C. elegans glia across sexes |
title | Molecular heterogeneity of C. elegans glia across sexes |
title_full | Molecular heterogeneity of C. elegans glia across sexes |
title_fullStr | Molecular heterogeneity of C. elegans glia across sexes |
title_full_unstemmed | Molecular heterogeneity of C. elegans glia across sexes |
title_short | Molecular heterogeneity of C. elegans glia across sexes |
title_sort | molecular heterogeneity of c. elegans glia across sexes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10055349/ https://www.ncbi.nlm.nih.gov/pubmed/36993469 http://dx.doi.org/10.1101/2023.03.21.533668 |
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