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Parabrachial nucleus activity in nociception and pain in awake mice

The parabrachial nuclear complex (PBN) is a nexus for aversion, and for the sensory and affective components of pain perception. We have previously shown that, during chronic pain, PBN neurons in anesthetized rodents have amplified activity. We report a method to record from PBN neurons of behaving,...

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Autores principales: Smith, Jesse Andrew, Ji, Yadong, Lorsung, Rebecca, Breault, Macauley Smith, Koenig, Jeffrey, Cramer, Nathan, Masri, Radi, Keller, Asaf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10055376/
https://www.ncbi.nlm.nih.gov/pubmed/36993729
http://dx.doi.org/10.1101/2023.03.22.533230
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author Smith, Jesse Andrew
Ji, Yadong
Lorsung, Rebecca
Breault, Macauley Smith
Koenig, Jeffrey
Cramer, Nathan
Masri, Radi
Keller, Asaf
author_facet Smith, Jesse Andrew
Ji, Yadong
Lorsung, Rebecca
Breault, Macauley Smith
Koenig, Jeffrey
Cramer, Nathan
Masri, Radi
Keller, Asaf
author_sort Smith, Jesse Andrew
collection PubMed
description The parabrachial nuclear complex (PBN) is a nexus for aversion, and for the sensory and affective components of pain perception. We have previously shown that, during chronic pain, PBN neurons in anesthetized rodents have amplified activity. We report a method to record from PBN neurons of behaving, head-restrained mice, while applying reproducible noxious stimuli. We find that both spontaneous and evoked activity are higher in awake animals, compared to urethane anesthetized mice. Fiber photometry of calcium responses from CGRP-expressing PBN neurons demonstrates that these neurons respond to nociceptive stimuli. In both males and females with neuropathic or inflammatory pain, responses of PBN neurons remain amplified for at least 5 weeks, in parallel with increased pain metrics. We also show that PBN neurons can be rapidly conditioned to respond to innocuous stimuli, after pairing with nociceptive stimuli. Finally, we demonstrate that changes in PBN neuronal activity are correlated with changes in arousal, measured as changes in pupil diameter.
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spelling pubmed-100553762023-03-30 Parabrachial nucleus activity in nociception and pain in awake mice Smith, Jesse Andrew Ji, Yadong Lorsung, Rebecca Breault, Macauley Smith Koenig, Jeffrey Cramer, Nathan Masri, Radi Keller, Asaf bioRxiv Article The parabrachial nuclear complex (PBN) is a nexus for aversion, and for the sensory and affective components of pain perception. We have previously shown that, during chronic pain, PBN neurons in anesthetized rodents have amplified activity. We report a method to record from PBN neurons of behaving, head-restrained mice, while applying reproducible noxious stimuli. We find that both spontaneous and evoked activity are higher in awake animals, compared to urethane anesthetized mice. Fiber photometry of calcium responses from CGRP-expressing PBN neurons demonstrates that these neurons respond to nociceptive stimuli. In both males and females with neuropathic or inflammatory pain, responses of PBN neurons remain amplified for at least 5 weeks, in parallel with increased pain metrics. We also show that PBN neurons can be rapidly conditioned to respond to innocuous stimuli, after pairing with nociceptive stimuli. Finally, we demonstrate that changes in PBN neuronal activity are correlated with changes in arousal, measured as changes in pupil diameter. Cold Spring Harbor Laboratory 2023-05-29 /pmc/articles/PMC10055376/ /pubmed/36993729 http://dx.doi.org/10.1101/2023.03.22.533230 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator.
spellingShingle Article
Smith, Jesse Andrew
Ji, Yadong
Lorsung, Rebecca
Breault, Macauley Smith
Koenig, Jeffrey
Cramer, Nathan
Masri, Radi
Keller, Asaf
Parabrachial nucleus activity in nociception and pain in awake mice
title Parabrachial nucleus activity in nociception and pain in awake mice
title_full Parabrachial nucleus activity in nociception and pain in awake mice
title_fullStr Parabrachial nucleus activity in nociception and pain in awake mice
title_full_unstemmed Parabrachial nucleus activity in nociception and pain in awake mice
title_short Parabrachial nucleus activity in nociception and pain in awake mice
title_sort parabrachial nucleus activity in nociception and pain in awake mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10055376/
https://www.ncbi.nlm.nih.gov/pubmed/36993729
http://dx.doi.org/10.1101/2023.03.22.533230
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