A metagenomic library cloning strategy that promotes high-level expression of captured genes to enable efficient functional screening

Functional screening of environmental DNA (eDNA) libraries is a potentially powerful approach to discover enzymatic “unknown unknowns”, but is usually heavily biased toward the tiny subset of genes preferentially transcribed and translated by the screening strain. We have overcome this by preparing...

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Detalles Bibliográficos
Autores principales: Rich, Michelle H, Sharrock, Abigail V, Mulligan, Timothy S, Matthews, Frazer, Brown, Alistair S, Lee-Harwood, Hannah R, Williams, Elsie M, Copp, Janine N, Little, Rory F, Francis, Jenni JB, Horvat, Claire N, Stevenson, Luke J, Owen, Jeremy G, Saxena, Meera T, Mumm, Jeff S, Ackerley, David F
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10055417/
https://www.ncbi.nlm.nih.gov/pubmed/36993673
http://dx.doi.org/10.1101/2023.03.24.534183

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