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Spinal sensorimotor circuits play a prominent role in hindlimb locomotor recovery after staggered thoracic lateral hemisections but cannot restore posture and interlimb coordination during quadrupedal locomotion in adult cats

Spinal sensorimotor circuits interact with supraspinal and peripheral inputs to generate quadrupedal locomotion. Ascending and descending spinal pathways ensure coordination between the fore- and hindlimbs. Spinal cord injury disrupts these pathways. To investigate the control of interlimb coordinat...

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Autores principales: Audet, Johannie, Yassine, Sirine, Lecomte, Charly G, Mari, Stephen, Félix, Soucy, Caroline, Morency, Merlet, Angèle N, Harnie, Jonathan, Beaulieu, Claudie, Gendron, Louis, Rybak, Ilya A., Prilutsky, Boris I., Frigon, Alain
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10055434/
https://www.ncbi.nlm.nih.gov/pubmed/36993268
http://dx.doi.org/10.1101/2023.03.23.533936
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author Audet, Johannie
Yassine, Sirine
Lecomte, Charly G
Mari, Stephen
Félix, Soucy
Caroline, Morency
Merlet, Angèle N
Harnie, Jonathan
Beaulieu, Claudie
Gendron, Louis
Rybak, Ilya A.
Prilutsky, Boris I.
Frigon, Alain
author_facet Audet, Johannie
Yassine, Sirine
Lecomte, Charly G
Mari, Stephen
Félix, Soucy
Caroline, Morency
Merlet, Angèle N
Harnie, Jonathan
Beaulieu, Claudie
Gendron, Louis
Rybak, Ilya A.
Prilutsky, Boris I.
Frigon, Alain
author_sort Audet, Johannie
collection PubMed
description Spinal sensorimotor circuits interact with supraspinal and peripheral inputs to generate quadrupedal locomotion. Ascending and descending spinal pathways ensure coordination between the fore- and hindlimbs. Spinal cord injury disrupts these pathways. To investigate the control of interlimb coordination and hindlimb locomotor recovery, we performed two lateral thoracic hemisections placed on opposite sides of the cord (right T5-T6 and left T10-T11) at an interval of approximately two months in eight adult cats. In three cats, we then made a complete spinal transection caudal to the second hemisection at T12-T13. We collected electromyography and kinematic data during quadrupedal and hindlimb-only locomotion before and after spinal lesions. We show that 1) cats spontaneously recover quadrupedal locomotion following staggered hemisections but require balance assistance after the second one, 2) coordination between the fore- and hindlimbs displays 2:1 patterns and becomes weaker and more variable after both hemisections, 3) left-right asymmetries in hindlimb stance and swing durations appear after the first hemisection and reverse after the second, and 4) support periods reorganize after staggered hemisections to favor support involving both forelimbs and diagonal limbs. Cats expressed hindlimb locomotion the day following spinal transection, indicating that lumbar sensorimotor circuits play a prominent role in hindlimb locomotor recovery after staggered hemisections. These results reflect a series of changes in spinal sensorimotor circuits that allow cats to maintain and recover some level of quadrupedal locomotor functionality with diminished motor commands from the brain and cervical cord, although the control of posture and interlimb coordination remains impaired.
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spelling pubmed-100554342023-03-30 Spinal sensorimotor circuits play a prominent role in hindlimb locomotor recovery after staggered thoracic lateral hemisections but cannot restore posture and interlimb coordination during quadrupedal locomotion in adult cats Audet, Johannie Yassine, Sirine Lecomte, Charly G Mari, Stephen Félix, Soucy Caroline, Morency Merlet, Angèle N Harnie, Jonathan Beaulieu, Claudie Gendron, Louis Rybak, Ilya A. Prilutsky, Boris I. Frigon, Alain bioRxiv Article Spinal sensorimotor circuits interact with supraspinal and peripheral inputs to generate quadrupedal locomotion. Ascending and descending spinal pathways ensure coordination between the fore- and hindlimbs. Spinal cord injury disrupts these pathways. To investigate the control of interlimb coordination and hindlimb locomotor recovery, we performed two lateral thoracic hemisections placed on opposite sides of the cord (right T5-T6 and left T10-T11) at an interval of approximately two months in eight adult cats. In three cats, we then made a complete spinal transection caudal to the second hemisection at T12-T13. We collected electromyography and kinematic data during quadrupedal and hindlimb-only locomotion before and after spinal lesions. We show that 1) cats spontaneously recover quadrupedal locomotion following staggered hemisections but require balance assistance after the second one, 2) coordination between the fore- and hindlimbs displays 2:1 patterns and becomes weaker and more variable after both hemisections, 3) left-right asymmetries in hindlimb stance and swing durations appear after the first hemisection and reverse after the second, and 4) support periods reorganize after staggered hemisections to favor support involving both forelimbs and diagonal limbs. Cats expressed hindlimb locomotion the day following spinal transection, indicating that lumbar sensorimotor circuits play a prominent role in hindlimb locomotor recovery after staggered hemisections. These results reflect a series of changes in spinal sensorimotor circuits that allow cats to maintain and recover some level of quadrupedal locomotor functionality with diminished motor commands from the brain and cervical cord, although the control of posture and interlimb coordination remains impaired. Cold Spring Harbor Laboratory 2023-03-25 /pmc/articles/PMC10055434/ /pubmed/36993268 http://dx.doi.org/10.1101/2023.03.23.533936 Text en https://creativecommons.org/licenses/by-nd/4.0/This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, and only so long as attribution is given to the creator. The license allows for commercial use.
spellingShingle Article
Audet, Johannie
Yassine, Sirine
Lecomte, Charly G
Mari, Stephen
Félix, Soucy
Caroline, Morency
Merlet, Angèle N
Harnie, Jonathan
Beaulieu, Claudie
Gendron, Louis
Rybak, Ilya A.
Prilutsky, Boris I.
Frigon, Alain
Spinal sensorimotor circuits play a prominent role in hindlimb locomotor recovery after staggered thoracic lateral hemisections but cannot restore posture and interlimb coordination during quadrupedal locomotion in adult cats
title Spinal sensorimotor circuits play a prominent role in hindlimb locomotor recovery after staggered thoracic lateral hemisections but cannot restore posture and interlimb coordination during quadrupedal locomotion in adult cats
title_full Spinal sensorimotor circuits play a prominent role in hindlimb locomotor recovery after staggered thoracic lateral hemisections but cannot restore posture and interlimb coordination during quadrupedal locomotion in adult cats
title_fullStr Spinal sensorimotor circuits play a prominent role in hindlimb locomotor recovery after staggered thoracic lateral hemisections but cannot restore posture and interlimb coordination during quadrupedal locomotion in adult cats
title_full_unstemmed Spinal sensorimotor circuits play a prominent role in hindlimb locomotor recovery after staggered thoracic lateral hemisections but cannot restore posture and interlimb coordination during quadrupedal locomotion in adult cats
title_short Spinal sensorimotor circuits play a prominent role in hindlimb locomotor recovery after staggered thoracic lateral hemisections but cannot restore posture and interlimb coordination during quadrupedal locomotion in adult cats
title_sort spinal sensorimotor circuits play a prominent role in hindlimb locomotor recovery after staggered thoracic lateral hemisections but cannot restore posture and interlimb coordination during quadrupedal locomotion in adult cats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10055434/
https://www.ncbi.nlm.nih.gov/pubmed/36993268
http://dx.doi.org/10.1101/2023.03.23.533936
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