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Glycerol Droplet Spreading on Growing Bacillus Subtilis Biofilms

Bacterial biofilm is a three-dimensional matrix composed of a large number of living bacterial individuals. The strong bio-interaction between the bacteria and its self-secreted matrix environment strengthens the mechanical integrity of the biofilm and the sustainable resistance of bacteria to antib...

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Autores principales: Luo, Siyang, Liu, Yanan, Luo, Hao, Jing, Guangyin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10055872/
https://www.ncbi.nlm.nih.gov/pubmed/36985005
http://dx.doi.org/10.3390/mi14030599
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author Luo, Siyang
Liu, Yanan
Luo, Hao
Jing, Guangyin
author_facet Luo, Siyang
Liu, Yanan
Luo, Hao
Jing, Guangyin
author_sort Luo, Siyang
collection PubMed
description Bacterial biofilm is a three-dimensional matrix composed of a large number of living bacterial individuals. The strong bio-interaction between the bacteria and its self-secreted matrix environment strengthens the mechanical integrity of the biofilm and the sustainable resistance of bacteria to antibiotics. As a soft surface, the biofilm is expected to present different dynamical wetting behavior in response to shear stress, which is, however, less known. Here, the spreading of liquid droplet on Bacillus subtilis biofilm at its different growing phases was experimentally investigated. Due to the viscoelastic response of the biofilm to fast spreading of the droplet, three stages were identified as inertial, viscous stages, and a longer transition in between. The physical heterogeneity of growing biofilm correlates with the spreading scaling within the inertial stage, followed by the possible chemical variation after a critical growing time. By using the duration of inertial spreading, the characteristic time scale was successfully linked to the shear modulus of the elastic dissipation of the biofilm. This measurement suggests a facile, non-destructive and in vivo method to understand the mechanical instability of this living matter.
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spelling pubmed-100558722023-03-30 Glycerol Droplet Spreading on Growing Bacillus Subtilis Biofilms Luo, Siyang Liu, Yanan Luo, Hao Jing, Guangyin Micromachines (Basel) Communication Bacterial biofilm is a three-dimensional matrix composed of a large number of living bacterial individuals. The strong bio-interaction between the bacteria and its self-secreted matrix environment strengthens the mechanical integrity of the biofilm and the sustainable resistance of bacteria to antibiotics. As a soft surface, the biofilm is expected to present different dynamical wetting behavior in response to shear stress, which is, however, less known. Here, the spreading of liquid droplet on Bacillus subtilis biofilm at its different growing phases was experimentally investigated. Due to the viscoelastic response of the biofilm to fast spreading of the droplet, three stages were identified as inertial, viscous stages, and a longer transition in between. The physical heterogeneity of growing biofilm correlates with the spreading scaling within the inertial stage, followed by the possible chemical variation after a critical growing time. By using the duration of inertial spreading, the characteristic time scale was successfully linked to the shear modulus of the elastic dissipation of the biofilm. This measurement suggests a facile, non-destructive and in vivo method to understand the mechanical instability of this living matter. MDPI 2023-03-04 /pmc/articles/PMC10055872/ /pubmed/36985005 http://dx.doi.org/10.3390/mi14030599 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Luo, Siyang
Liu, Yanan
Luo, Hao
Jing, Guangyin
Glycerol Droplet Spreading on Growing Bacillus Subtilis Biofilms
title Glycerol Droplet Spreading on Growing Bacillus Subtilis Biofilms
title_full Glycerol Droplet Spreading on Growing Bacillus Subtilis Biofilms
title_fullStr Glycerol Droplet Spreading on Growing Bacillus Subtilis Biofilms
title_full_unstemmed Glycerol Droplet Spreading on Growing Bacillus Subtilis Biofilms
title_short Glycerol Droplet Spreading on Growing Bacillus Subtilis Biofilms
title_sort glycerol droplet spreading on growing bacillus subtilis biofilms
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10055872/
https://www.ncbi.nlm.nih.gov/pubmed/36985005
http://dx.doi.org/10.3390/mi14030599
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