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Molecular Characterization of the First African Swine Fever Virus Genotype II Strains Identified from Mainland Italy, 2022

African swine fever (ASF) is responsible for important socio-economic effects in the global pig industry, especially for countries with large-scale piggery sectors. In January 2022, the African swine fever virus (ASFV) genotype II was identified in a wild boar population in mainland Italy (Piedmont...

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Autores principales: Giammarioli, Monica, Alessandro, Dondo, Cammà, Cesare, Masoero, Loretta, Torresi, Claudia, Marcacci, Maurilia, Zoppi, Simona, Curini, Valentina, Rinaldi, Antonio, Rossi, Elisabetta, Casciari, Cristina, Pela, Michela, Pellegrini, Claudia, Iscaro, Carmen, Feliziani, Francesco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10055901/
https://www.ncbi.nlm.nih.gov/pubmed/36986294
http://dx.doi.org/10.3390/pathogens12030372
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author Giammarioli, Monica
Alessandro, Dondo
Cammà, Cesare
Masoero, Loretta
Torresi, Claudia
Marcacci, Maurilia
Zoppi, Simona
Curini, Valentina
Rinaldi, Antonio
Rossi, Elisabetta
Casciari, Cristina
Pela, Michela
Pellegrini, Claudia
Iscaro, Carmen
Feliziani, Francesco
author_facet Giammarioli, Monica
Alessandro, Dondo
Cammà, Cesare
Masoero, Loretta
Torresi, Claudia
Marcacci, Maurilia
Zoppi, Simona
Curini, Valentina
Rinaldi, Antonio
Rossi, Elisabetta
Casciari, Cristina
Pela, Michela
Pellegrini, Claudia
Iscaro, Carmen
Feliziani, Francesco
author_sort Giammarioli, Monica
collection PubMed
description African swine fever (ASF) is responsible for important socio-economic effects in the global pig industry, especially for countries with large-scale piggery sectors. In January 2022, the African swine fever virus (ASFV) genotype II was identified in a wild boar population in mainland Italy (Piedmont region). This study describes the molecular characterization, by Sanger and next-generation sequencing (NGS), of the first index case 632/AL/2022 and of another isolate (2802/AL/2022) reported in the same month, in close proximity to the first, following multiple ASF outbreaks. Phylogenetic analysis based on the B646L gene and NGS clustered the isolates 632/AL/2022 and 2802/AL/2022 within the wide and most homogeneous p72 genotype II that includes viruses from European and Asian countries. The consensus sequence obtained from the ASFV 2802/AL/2022 isolate was 190,598 nucleotides in length and had a mean GC content of 38.38%. At the whole-genome level, ASF isolate 2802/AL/2022 showed a close genetic correlation with the other representative ASFV genotype II strains isolated between April 2007 and January 2022 from wild and domestic pigs in Eastern/Central European (EU) and Asian countries. CVR subtyping clustered the two Italian ASFV strains within the major CVR variant circulating since the first virus introduction in Georgia in 2007. Intergenic region I73R-I329L subtyping placed the Italian ASFV isolates within the variant identical to the strains frequently identified among wild boars and domestic pigs. Presently, given the high sequence similarity, it is impossible to trace the precise geographic origin of the virus at a country level. Moreover, the full-length sequences available in the NCBI are not completely representative of all affected territories.
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spelling pubmed-100559012023-03-30 Molecular Characterization of the First African Swine Fever Virus Genotype II Strains Identified from Mainland Italy, 2022 Giammarioli, Monica Alessandro, Dondo Cammà, Cesare Masoero, Loretta Torresi, Claudia Marcacci, Maurilia Zoppi, Simona Curini, Valentina Rinaldi, Antonio Rossi, Elisabetta Casciari, Cristina Pela, Michela Pellegrini, Claudia Iscaro, Carmen Feliziani, Francesco Pathogens Article African swine fever (ASF) is responsible for important socio-economic effects in the global pig industry, especially for countries with large-scale piggery sectors. In January 2022, the African swine fever virus (ASFV) genotype II was identified in a wild boar population in mainland Italy (Piedmont region). This study describes the molecular characterization, by Sanger and next-generation sequencing (NGS), of the first index case 632/AL/2022 and of another isolate (2802/AL/2022) reported in the same month, in close proximity to the first, following multiple ASF outbreaks. Phylogenetic analysis based on the B646L gene and NGS clustered the isolates 632/AL/2022 and 2802/AL/2022 within the wide and most homogeneous p72 genotype II that includes viruses from European and Asian countries. The consensus sequence obtained from the ASFV 2802/AL/2022 isolate was 190,598 nucleotides in length and had a mean GC content of 38.38%. At the whole-genome level, ASF isolate 2802/AL/2022 showed a close genetic correlation with the other representative ASFV genotype II strains isolated between April 2007 and January 2022 from wild and domestic pigs in Eastern/Central European (EU) and Asian countries. CVR subtyping clustered the two Italian ASFV strains within the major CVR variant circulating since the first virus introduction in Georgia in 2007. Intergenic region I73R-I329L subtyping placed the Italian ASFV isolates within the variant identical to the strains frequently identified among wild boars and domestic pigs. Presently, given the high sequence similarity, it is impossible to trace the precise geographic origin of the virus at a country level. Moreover, the full-length sequences available in the NCBI are not completely representative of all affected territories. MDPI 2023-02-24 /pmc/articles/PMC10055901/ /pubmed/36986294 http://dx.doi.org/10.3390/pathogens12030372 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Giammarioli, Monica
Alessandro, Dondo
Cammà, Cesare
Masoero, Loretta
Torresi, Claudia
Marcacci, Maurilia
Zoppi, Simona
Curini, Valentina
Rinaldi, Antonio
Rossi, Elisabetta
Casciari, Cristina
Pela, Michela
Pellegrini, Claudia
Iscaro, Carmen
Feliziani, Francesco
Molecular Characterization of the First African Swine Fever Virus Genotype II Strains Identified from Mainland Italy, 2022
title Molecular Characterization of the First African Swine Fever Virus Genotype II Strains Identified from Mainland Italy, 2022
title_full Molecular Characterization of the First African Swine Fever Virus Genotype II Strains Identified from Mainland Italy, 2022
title_fullStr Molecular Characterization of the First African Swine Fever Virus Genotype II Strains Identified from Mainland Italy, 2022
title_full_unstemmed Molecular Characterization of the First African Swine Fever Virus Genotype II Strains Identified from Mainland Italy, 2022
title_short Molecular Characterization of the First African Swine Fever Virus Genotype II Strains Identified from Mainland Italy, 2022
title_sort molecular characterization of the first african swine fever virus genotype ii strains identified from mainland italy, 2022
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10055901/
https://www.ncbi.nlm.nih.gov/pubmed/36986294
http://dx.doi.org/10.3390/pathogens12030372
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