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Evaluation of an Artificial Mouth for Dental Caries Development

This study validated a microbial caries model (artificial mouth) for dental caries development to determine the optimal time to create early caries suitable for evaluation of the efficacy of caries therapeutic agents. In all, 40 human enamel blocks were placed in an artificial mouth at 37 °C and 5%...

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Autores principales: Amaechi, Bennett T., Abdul Azees, Parveez Ahamed, Farah, Rayane, Movaghari Pour, Fatemeh, Dillow, Andrew M., Lin, Chun-Yen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10056027/
https://www.ncbi.nlm.nih.gov/pubmed/36985202
http://dx.doi.org/10.3390/microorganisms11030628
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author Amaechi, Bennett T.
Abdul Azees, Parveez Ahamed
Farah, Rayane
Movaghari Pour, Fatemeh
Dillow, Andrew M.
Lin, Chun-Yen
author_facet Amaechi, Bennett T.
Abdul Azees, Parveez Ahamed
Farah, Rayane
Movaghari Pour, Fatemeh
Dillow, Andrew M.
Lin, Chun-Yen
author_sort Amaechi, Bennett T.
collection PubMed
description This study validated a microbial caries model (artificial mouth) for dental caries development to determine the optimal time to create early caries suitable for evaluation of the efficacy of caries therapeutic agents. In all, 40 human enamel blocks were placed in an artificial mouth at 37 °C and 5% CO(2) and were exposed to brain heart infusion broth inoculated with S. mutans in continuous circulation (0.3 mL/min). The culture medium was replaced three times daily. Samples were exposed to 10% sucrose for 3 min, 3 times daily to promote biofilm growth. Five samples were harvested from the chamber after 3, 4, 5, 6, 7, 14, 21, and 28 days. At the end of experiment, samples were assessed visually by ICDAS criteria, while lesion depth (LD) and mineral loss (ML) were measured using polarizing light microscopy and transverse microradiography. Data were analyzed by Pearson correlation, ANOVA, and Tukey comparison test (p < 0.05). Results showed significant and strong positive correlation (p < 0.01) between all variables and biofilm growth time. LD and ML profiles of 7-day lesions seem to be the most suitable for remineralization studies. In conclusion, using the evaluated artificial mouth, early-stage caries suitable for products’ evaluation studies was produced within 7 days of exposure to microbial biofilm.
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spelling pubmed-100560272023-03-30 Evaluation of an Artificial Mouth for Dental Caries Development Amaechi, Bennett T. Abdul Azees, Parveez Ahamed Farah, Rayane Movaghari Pour, Fatemeh Dillow, Andrew M. Lin, Chun-Yen Microorganisms Article This study validated a microbial caries model (artificial mouth) for dental caries development to determine the optimal time to create early caries suitable for evaluation of the efficacy of caries therapeutic agents. In all, 40 human enamel blocks were placed in an artificial mouth at 37 °C and 5% CO(2) and were exposed to brain heart infusion broth inoculated with S. mutans in continuous circulation (0.3 mL/min). The culture medium was replaced three times daily. Samples were exposed to 10% sucrose for 3 min, 3 times daily to promote biofilm growth. Five samples were harvested from the chamber after 3, 4, 5, 6, 7, 14, 21, and 28 days. At the end of experiment, samples were assessed visually by ICDAS criteria, while lesion depth (LD) and mineral loss (ML) were measured using polarizing light microscopy and transverse microradiography. Data were analyzed by Pearson correlation, ANOVA, and Tukey comparison test (p < 0.05). Results showed significant and strong positive correlation (p < 0.01) between all variables and biofilm growth time. LD and ML profiles of 7-day lesions seem to be the most suitable for remineralization studies. In conclusion, using the evaluated artificial mouth, early-stage caries suitable for products’ evaluation studies was produced within 7 days of exposure to microbial biofilm. MDPI 2023-02-28 /pmc/articles/PMC10056027/ /pubmed/36985202 http://dx.doi.org/10.3390/microorganisms11030628 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Amaechi, Bennett T.
Abdul Azees, Parveez Ahamed
Farah, Rayane
Movaghari Pour, Fatemeh
Dillow, Andrew M.
Lin, Chun-Yen
Evaluation of an Artificial Mouth for Dental Caries Development
title Evaluation of an Artificial Mouth for Dental Caries Development
title_full Evaluation of an Artificial Mouth for Dental Caries Development
title_fullStr Evaluation of an Artificial Mouth for Dental Caries Development
title_full_unstemmed Evaluation of an Artificial Mouth for Dental Caries Development
title_short Evaluation of an Artificial Mouth for Dental Caries Development
title_sort evaluation of an artificial mouth for dental caries development
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10056027/
https://www.ncbi.nlm.nih.gov/pubmed/36985202
http://dx.doi.org/10.3390/microorganisms11030628
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