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Storage and Algal Association of Bacteria That Protect Microchloropsis salina from Grazing by Brachionus plicatilis

Loss of algal production from the crashes of algal mass cultivation systems represents a significant barrier to the economic production of microalgal-based biofuels. Current strategies for crash prevention can be too costly to apply broadly as prophylaxis. Bacteria are ubiquitous in microalgal mass...

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Autores principales: Fisher, Carolyn L., Fong, Michelle V., Lane, Pamela D., Carlson, Skylar, Lane, Todd W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10056100/
https://www.ncbi.nlm.nih.gov/pubmed/36985359
http://dx.doi.org/10.3390/microorganisms11030786
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author Fisher, Carolyn L.
Fong, Michelle V.
Lane, Pamela D.
Carlson, Skylar
Lane, Todd W.
author_facet Fisher, Carolyn L.
Fong, Michelle V.
Lane, Pamela D.
Carlson, Skylar
Lane, Todd W.
author_sort Fisher, Carolyn L.
collection PubMed
description Loss of algal production from the crashes of algal mass cultivation systems represents a significant barrier to the economic production of microalgal-based biofuels. Current strategies for crash prevention can be too costly to apply broadly as prophylaxis. Bacteria are ubiquitous in microalgal mass production cultures, however few studies investigate their role and possible significance in this particular environment. Previously, we demonstrated the success of selected protective bacterial communities to save Microchloropsis salina cultures from grazing by the rotifer Brachionus plicatilis. In the current study, these protective bacterial communities were further characterized by fractionation into rotifer-associated, algal-associated, and free-floating bacterial fractions. Small subunit ribosomal RNA amplicon sequencing was used to identify the bacterial genera present in each of the fractions. Here, we show that Marinobacter, Ruegeria, and Boseongicola in algae and rotifer fractions from rotifer-infected cultures likely play key roles in protecting algae from rotifers. Several other identified taxa likely play lesser roles in protective capability. The identification of bacterial community members demonstrating protective qualities will allow for the rational design of microbial communities grown in stable co-cultures with algal production strains in mass cultivation systems. Such a system would reduce the frequency of culture crashes and represent an essentially zero-cost form of algal crop protection.
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spelling pubmed-100561002023-03-30 Storage and Algal Association of Bacteria That Protect Microchloropsis salina from Grazing by Brachionus plicatilis Fisher, Carolyn L. Fong, Michelle V. Lane, Pamela D. Carlson, Skylar Lane, Todd W. Microorganisms Communication Loss of algal production from the crashes of algal mass cultivation systems represents a significant barrier to the economic production of microalgal-based biofuels. Current strategies for crash prevention can be too costly to apply broadly as prophylaxis. Bacteria are ubiquitous in microalgal mass production cultures, however few studies investigate their role and possible significance in this particular environment. Previously, we demonstrated the success of selected protective bacterial communities to save Microchloropsis salina cultures from grazing by the rotifer Brachionus plicatilis. In the current study, these protective bacterial communities were further characterized by fractionation into rotifer-associated, algal-associated, and free-floating bacterial fractions. Small subunit ribosomal RNA amplicon sequencing was used to identify the bacterial genera present in each of the fractions. Here, we show that Marinobacter, Ruegeria, and Boseongicola in algae and rotifer fractions from rotifer-infected cultures likely play key roles in protecting algae from rotifers. Several other identified taxa likely play lesser roles in protective capability. The identification of bacterial community members demonstrating protective qualities will allow for the rational design of microbial communities grown in stable co-cultures with algal production strains in mass cultivation systems. Such a system would reduce the frequency of culture crashes and represent an essentially zero-cost form of algal crop protection. MDPI 2023-03-18 /pmc/articles/PMC10056100/ /pubmed/36985359 http://dx.doi.org/10.3390/microorganisms11030786 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Fisher, Carolyn L.
Fong, Michelle V.
Lane, Pamela D.
Carlson, Skylar
Lane, Todd W.
Storage and Algal Association of Bacteria That Protect Microchloropsis salina from Grazing by Brachionus plicatilis
title Storage and Algal Association of Bacteria That Protect Microchloropsis salina from Grazing by Brachionus plicatilis
title_full Storage and Algal Association of Bacteria That Protect Microchloropsis salina from Grazing by Brachionus plicatilis
title_fullStr Storage and Algal Association of Bacteria That Protect Microchloropsis salina from Grazing by Brachionus plicatilis
title_full_unstemmed Storage and Algal Association of Bacteria That Protect Microchloropsis salina from Grazing by Brachionus plicatilis
title_short Storage and Algal Association of Bacteria That Protect Microchloropsis salina from Grazing by Brachionus plicatilis
title_sort storage and algal association of bacteria that protect microchloropsis salina from grazing by brachionus plicatilis
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10056100/
https://www.ncbi.nlm.nih.gov/pubmed/36985359
http://dx.doi.org/10.3390/microorganisms11030786
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