Cargando…

Volatile Constituents in Essential Oil from Leaves of Withania adpressa Coss. Ex Exhibit Potent Antioxidant and Antimicrobial Properties against Clinically-Relevant Pathogens

Withania adpressa Coss. ex is a plant used in traditional medications. Antioxidant, antibacterial, and antifungal properties of the essential oil from leaves of Withania adpressa Coss ex. (EOW) were investigated. EOW was extracted using a Clevenger apparatus, and its volatile compounds were characte...

Descripción completa

Detalles Bibliográficos
Autores principales: Bourhia, Mohammed, Alyousef, Abdullah A., Doumane, Ghizlane, Saghrouchni, Hamza, Giesy, John P., Ouahmane, Lahcen, Gueddari, Fatiha EL, Al-Sheikh, Yazeed A., Aboul-Soud, Mourad A. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10056193/
https://www.ncbi.nlm.nih.gov/pubmed/36985810
http://dx.doi.org/10.3390/molecules28062839
_version_ 1785016065086980096
author Bourhia, Mohammed
Alyousef, Abdullah A.
Doumane, Ghizlane
Saghrouchni, Hamza
Giesy, John P.
Ouahmane, Lahcen
Gueddari, Fatiha EL
Al-Sheikh, Yazeed A.
Aboul-Soud, Mourad A. M.
author_facet Bourhia, Mohammed
Alyousef, Abdullah A.
Doumane, Ghizlane
Saghrouchni, Hamza
Giesy, John P.
Ouahmane, Lahcen
Gueddari, Fatiha EL
Al-Sheikh, Yazeed A.
Aboul-Soud, Mourad A. M.
author_sort Bourhia, Mohammed
collection PubMed
description Withania adpressa Coss. ex is a plant used in traditional medications. Antioxidant, antibacterial, and antifungal properties of the essential oil from leaves of Withania adpressa Coss ex. (EOW) were investigated. EOW was extracted using a Clevenger apparatus, and its volatile compounds were characterized by GC-MS. Antioxidant potency was determined using DPPH, FRAP, and TAC assays. Antibacterial effects were determined vs. Escherichia coli, Klebsiella pneumonia, Staphylococcus aureus, and Streptococcus pneumonia; while its antifungal efficacy was determined vs. Candida albicans, Aspergillus flavus, Aspergillus niger, and Fusarium oxysporum using the disc diffusion and minimum inhibitory concentration bioassays. A chromatographic analysis showed that EOW contained eight phytochemical compounds constituting 99.14% of the total mass of oil. Caryophyllene (24.74%), Longifolene (21.37%), δ-Cadinene (19.08%), and Carene (14.86%) were predominant compounds in EOW. The concentrations required to inhibit 50% of free radical (IC50) values of antioxidant activities of EOW were 0.031 ± 0.006 mg/mL (DPPH), 0.011 ± 0.003 mg/mL (FRAP), and 846.25 ± 1.07 mg AAE/g (TAC). Inhibition zone diameters of EOW vs. bacteria were 18.11 ± 0.5 mm (E. coli), 17.10 ± 0.42 mm (S. aureus), 12.13 ± 0.31 mm (K. pneumoniae), and 11.09 ± 0.47 mm (S. pneumoniae), while MIC values were 51 ± 3, 47 ± 5, 46 ± 3 and 31 ± 1 µg/mL, respectively. Inhibition zone diameters of EOW vs. fungi were 31.32 ± 1.32, 29.00 ± 1.5, 27.63 ± 2.10, and 24.51 ± s1.07 mm for A. flavus, C. albicans, F. oxysporum, and A. niger, respectively. MIC values were 8.41 ± 0.40, 28.04 ± 0.26, 9.05 ± 0.76, and 22.26 ± 0.55 µg/mL, respectively. Importantly, the highest dose of EOW (1 mg/mL) showed negligible (~5%) cytotoxicity against MCF-12, a normal human epithelial cell line derived from the mammary gland, thus underscoring its wide safety and selectivity against tested microbes. To sum it up, EOW has exhibited promising antioxidant and antimicrobial properties, which suggests potential to abrogate antibiotic resistance.
format Online
Article
Text
id pubmed-10056193
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-100561932023-03-30 Volatile Constituents in Essential Oil from Leaves of Withania adpressa Coss. Ex Exhibit Potent Antioxidant and Antimicrobial Properties against Clinically-Relevant Pathogens Bourhia, Mohammed Alyousef, Abdullah A. Doumane, Ghizlane Saghrouchni, Hamza Giesy, John P. Ouahmane, Lahcen Gueddari, Fatiha EL Al-Sheikh, Yazeed A. Aboul-Soud, Mourad A. M. Molecules Article Withania adpressa Coss. ex is a plant used in traditional medications. Antioxidant, antibacterial, and antifungal properties of the essential oil from leaves of Withania adpressa Coss ex. (EOW) were investigated. EOW was extracted using a Clevenger apparatus, and its volatile compounds were characterized by GC-MS. Antioxidant potency was determined using DPPH, FRAP, and TAC assays. Antibacterial effects were determined vs. Escherichia coli, Klebsiella pneumonia, Staphylococcus aureus, and Streptococcus pneumonia; while its antifungal efficacy was determined vs. Candida albicans, Aspergillus flavus, Aspergillus niger, and Fusarium oxysporum using the disc diffusion and minimum inhibitory concentration bioassays. A chromatographic analysis showed that EOW contained eight phytochemical compounds constituting 99.14% of the total mass of oil. Caryophyllene (24.74%), Longifolene (21.37%), δ-Cadinene (19.08%), and Carene (14.86%) were predominant compounds in EOW. The concentrations required to inhibit 50% of free radical (IC50) values of antioxidant activities of EOW were 0.031 ± 0.006 mg/mL (DPPH), 0.011 ± 0.003 mg/mL (FRAP), and 846.25 ± 1.07 mg AAE/g (TAC). Inhibition zone diameters of EOW vs. bacteria were 18.11 ± 0.5 mm (E. coli), 17.10 ± 0.42 mm (S. aureus), 12.13 ± 0.31 mm (K. pneumoniae), and 11.09 ± 0.47 mm (S. pneumoniae), while MIC values were 51 ± 3, 47 ± 5, 46 ± 3 and 31 ± 1 µg/mL, respectively. Inhibition zone diameters of EOW vs. fungi were 31.32 ± 1.32, 29.00 ± 1.5, 27.63 ± 2.10, and 24.51 ± s1.07 mm for A. flavus, C. albicans, F. oxysporum, and A. niger, respectively. MIC values were 8.41 ± 0.40, 28.04 ± 0.26, 9.05 ± 0.76, and 22.26 ± 0.55 µg/mL, respectively. Importantly, the highest dose of EOW (1 mg/mL) showed negligible (~5%) cytotoxicity against MCF-12, a normal human epithelial cell line derived from the mammary gland, thus underscoring its wide safety and selectivity against tested microbes. To sum it up, EOW has exhibited promising antioxidant and antimicrobial properties, which suggests potential to abrogate antibiotic resistance. MDPI 2023-03-21 /pmc/articles/PMC10056193/ /pubmed/36985810 http://dx.doi.org/10.3390/molecules28062839 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bourhia, Mohammed
Alyousef, Abdullah A.
Doumane, Ghizlane
Saghrouchni, Hamza
Giesy, John P.
Ouahmane, Lahcen
Gueddari, Fatiha EL
Al-Sheikh, Yazeed A.
Aboul-Soud, Mourad A. M.
Volatile Constituents in Essential Oil from Leaves of Withania adpressa Coss. Ex Exhibit Potent Antioxidant and Antimicrobial Properties against Clinically-Relevant Pathogens
title Volatile Constituents in Essential Oil from Leaves of Withania adpressa Coss. Ex Exhibit Potent Antioxidant and Antimicrobial Properties against Clinically-Relevant Pathogens
title_full Volatile Constituents in Essential Oil from Leaves of Withania adpressa Coss. Ex Exhibit Potent Antioxidant and Antimicrobial Properties against Clinically-Relevant Pathogens
title_fullStr Volatile Constituents in Essential Oil from Leaves of Withania adpressa Coss. Ex Exhibit Potent Antioxidant and Antimicrobial Properties against Clinically-Relevant Pathogens
title_full_unstemmed Volatile Constituents in Essential Oil from Leaves of Withania adpressa Coss. Ex Exhibit Potent Antioxidant and Antimicrobial Properties against Clinically-Relevant Pathogens
title_short Volatile Constituents in Essential Oil from Leaves of Withania adpressa Coss. Ex Exhibit Potent Antioxidant and Antimicrobial Properties against Clinically-Relevant Pathogens
title_sort volatile constituents in essential oil from leaves of withania adpressa coss. ex exhibit potent antioxidant and antimicrobial properties against clinically-relevant pathogens
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10056193/
https://www.ncbi.nlm.nih.gov/pubmed/36985810
http://dx.doi.org/10.3390/molecules28062839
work_keys_str_mv AT bourhiamohammed volatileconstituentsinessentialoilfromleavesofwithaniaadpressacossexexhibitpotentantioxidantandantimicrobialpropertiesagainstclinicallyrelevantpathogens
AT alyousefabdullaha volatileconstituentsinessentialoilfromleavesofwithaniaadpressacossexexhibitpotentantioxidantandantimicrobialpropertiesagainstclinicallyrelevantpathogens
AT doumaneghizlane volatileconstituentsinessentialoilfromleavesofwithaniaadpressacossexexhibitpotentantioxidantandantimicrobialpropertiesagainstclinicallyrelevantpathogens
AT saghrouchnihamza volatileconstituentsinessentialoilfromleavesofwithaniaadpressacossexexhibitpotentantioxidantandantimicrobialpropertiesagainstclinicallyrelevantpathogens
AT giesyjohnp volatileconstituentsinessentialoilfromleavesofwithaniaadpressacossexexhibitpotentantioxidantandantimicrobialpropertiesagainstclinicallyrelevantpathogens
AT ouahmanelahcen volatileconstituentsinessentialoilfromleavesofwithaniaadpressacossexexhibitpotentantioxidantandantimicrobialpropertiesagainstclinicallyrelevantpathogens
AT gueddarifatihael volatileconstituentsinessentialoilfromleavesofwithaniaadpressacossexexhibitpotentantioxidantandantimicrobialpropertiesagainstclinicallyrelevantpathogens
AT alsheikhyazeeda volatileconstituentsinessentialoilfromleavesofwithaniaadpressacossexexhibitpotentantioxidantandantimicrobialpropertiesagainstclinicallyrelevantpathogens
AT aboulsoudmouradam volatileconstituentsinessentialoilfromleavesofwithaniaadpressacossexexhibitpotentantioxidantandantimicrobialpropertiesagainstclinicallyrelevantpathogens