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Anaplasma and Ehrlichia Species in Ixodidae Ticks Collected from Two Regions of Bulgaria

The aim of the study was to determine prevalence of Anaplasmataceae-infected ticks in the Black Sea Coast and the Pleven regions of Bulgaria. A total of 350 ticks from different tick species were collected. Two hundred fifty-five ticks were removed from dogs in the Black Sea Coast region, and 95 Ixo...

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Autores principales: Stanilov, Iskren, Blazhev, Alexander, Miteva, Lyuba
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10056244/
https://www.ncbi.nlm.nih.gov/pubmed/36985168
http://dx.doi.org/10.3390/microorganisms11030594
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author Stanilov, Iskren
Blazhev, Alexander
Miteva, Lyuba
author_facet Stanilov, Iskren
Blazhev, Alexander
Miteva, Lyuba
author_sort Stanilov, Iskren
collection PubMed
description The aim of the study was to determine prevalence of Anaplasmataceae-infected ticks in the Black Sea Coast and the Pleven regions of Bulgaria. A total of 350 ticks from different tick species were collected. Two hundred fifty-five ticks were removed from dogs in the Black Sea Coast region, and 95 Ixodes ricinus ticks were collected by flagging vegetation with a white flannel cloth in two areas in the region of Pleven. After the DNA isolation of the ticks, a genus-specific polymerase chain reaction (PCR) was performed to identify Anaplasmataceae. Second PCRs were performed with species-specific primers to identify Ehrlichia canis (E. canis) and Anaplasma phagocytophilum (A. phagocytophilum). The results showed that 26.9% of the Ixodes ricinus ticks were infected with Anaplasmataceae in the Black Sea Coast region and 36.8% in the Pleven region. The infection with E. canis was detected in 35.7% and A. phagocytophilum in 25.0% of positive ticks from the Black Sea Coast region. In the Pleven region, 22.9% of ticks were positive for E. canis, while 42.9% were positive for A. phagocytophilum. The molecular identification of E. canis in ticks collected from Bulgaria was performed for the first time. In conclusion, the present study revealed a higher prevalence of ticks infected with Anaplasmataceae, particularly A. phagocytophilum, in the Pleven region than in the Black Sea Coast region.
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spelling pubmed-100562442023-03-30 Anaplasma and Ehrlichia Species in Ixodidae Ticks Collected from Two Regions of Bulgaria Stanilov, Iskren Blazhev, Alexander Miteva, Lyuba Microorganisms Article The aim of the study was to determine prevalence of Anaplasmataceae-infected ticks in the Black Sea Coast and the Pleven regions of Bulgaria. A total of 350 ticks from different tick species were collected. Two hundred fifty-five ticks were removed from dogs in the Black Sea Coast region, and 95 Ixodes ricinus ticks were collected by flagging vegetation with a white flannel cloth in two areas in the region of Pleven. After the DNA isolation of the ticks, a genus-specific polymerase chain reaction (PCR) was performed to identify Anaplasmataceae. Second PCRs were performed with species-specific primers to identify Ehrlichia canis (E. canis) and Anaplasma phagocytophilum (A. phagocytophilum). The results showed that 26.9% of the Ixodes ricinus ticks were infected with Anaplasmataceae in the Black Sea Coast region and 36.8% in the Pleven region. The infection with E. canis was detected in 35.7% and A. phagocytophilum in 25.0% of positive ticks from the Black Sea Coast region. In the Pleven region, 22.9% of ticks were positive for E. canis, while 42.9% were positive for A. phagocytophilum. The molecular identification of E. canis in ticks collected from Bulgaria was performed for the first time. In conclusion, the present study revealed a higher prevalence of ticks infected with Anaplasmataceae, particularly A. phagocytophilum, in the Pleven region than in the Black Sea Coast region. MDPI 2023-02-26 /pmc/articles/PMC10056244/ /pubmed/36985168 http://dx.doi.org/10.3390/microorganisms11030594 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Stanilov, Iskren
Blazhev, Alexander
Miteva, Lyuba
Anaplasma and Ehrlichia Species in Ixodidae Ticks Collected from Two Regions of Bulgaria
title Anaplasma and Ehrlichia Species in Ixodidae Ticks Collected from Two Regions of Bulgaria
title_full Anaplasma and Ehrlichia Species in Ixodidae Ticks Collected from Two Regions of Bulgaria
title_fullStr Anaplasma and Ehrlichia Species in Ixodidae Ticks Collected from Two Regions of Bulgaria
title_full_unstemmed Anaplasma and Ehrlichia Species in Ixodidae Ticks Collected from Two Regions of Bulgaria
title_short Anaplasma and Ehrlichia Species in Ixodidae Ticks Collected from Two Regions of Bulgaria
title_sort anaplasma and ehrlichia species in ixodidae ticks collected from two regions of bulgaria
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10056244/
https://www.ncbi.nlm.nih.gov/pubmed/36985168
http://dx.doi.org/10.3390/microorganisms11030594
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