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The Influence of Prenatal Exposure to Methamphetamine on the Development of Dopaminergic Neurons in the Ventral Midbrain
Methamphetamine, a highly addictive central nervous system (CNS) stimulant, is used worldwide as an anorexiant and attention enhancer. Methamphetamine use during pregnancy, even at therapeutic doses, may harm fetal development. Here, we examined whether exposure to methamphetamine affects the morpho...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10056332/ https://www.ncbi.nlm.nih.gov/pubmed/36982742 http://dx.doi.org/10.3390/ijms24065668 |
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author | Alsanie, Walaa F. Abdelrahman, Sherin Felimban, Raed I. Alkhatabi, Heba A. Gaber, Ahmed Alosimi, Ebtisam Abdulah Alhomrani, Majid Habeeballah, Hamza Hauser, Charlotte A. E. S. Alamri, Abdulhakeem Althobaiti, Aiysha Alsharif, Abdulaziz Alzahrani, Ahmed S. Al-Ghamdi, Mohammad S. Raafat, Bassem M. Alswat, Khaled A. Althobaiti, Yusuf S. Asiri, Yousif A. |
author_facet | Alsanie, Walaa F. Abdelrahman, Sherin Felimban, Raed I. Alkhatabi, Heba A. Gaber, Ahmed Alosimi, Ebtisam Abdulah Alhomrani, Majid Habeeballah, Hamza Hauser, Charlotte A. E. S. Alamri, Abdulhakeem Althobaiti, Aiysha Alsharif, Abdulaziz Alzahrani, Ahmed S. Al-Ghamdi, Mohammad S. Raafat, Bassem M. Alswat, Khaled A. Althobaiti, Yusuf S. Asiri, Yousif A. |
author_sort | Alsanie, Walaa F. |
collection | PubMed |
description | Methamphetamine, a highly addictive central nervous system (CNS) stimulant, is used worldwide as an anorexiant and attention enhancer. Methamphetamine use during pregnancy, even at therapeutic doses, may harm fetal development. Here, we examined whether exposure to methamphetamine affects the morphogenesis and diversity of ventral midbrain dopaminergic neurons (VMDNs). The effects of methamphetamine on morphogenesis, viability, the release of mediator chemicals (such as ATP), and the expression of genes involved in neurogenesis were evaluated using VMDNs isolated from the embryos of timed-mated mice on embryonic day 12.5. We demonstrated that methamphetamine (10 µM; equivalent to its therapeutic dose) did not affect the viability and morphogenesis of VMDNs, but it reduced the ATP release negligibly. It significantly downregulated Lmx1a, En1, Pitx3, Th, Chl1, Dat, and Drd1 but did not affect Nurr1 or Bdnf expression. Our results illustrate that methamphetamine could impair VMDN differentiation by altering the expression of important neurogenesis-related genes. Overall, this study suggests that methamphetamine use may impair VMDNs in the fetus if taken during pregnancy. Therefore, it is essential to exercise strict caution for its use in expectant mothers. |
format | Online Article Text |
id | pubmed-10056332 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100563322023-03-30 The Influence of Prenatal Exposure to Methamphetamine on the Development of Dopaminergic Neurons in the Ventral Midbrain Alsanie, Walaa F. Abdelrahman, Sherin Felimban, Raed I. Alkhatabi, Heba A. Gaber, Ahmed Alosimi, Ebtisam Abdulah Alhomrani, Majid Habeeballah, Hamza Hauser, Charlotte A. E. S. Alamri, Abdulhakeem Althobaiti, Aiysha Alsharif, Abdulaziz Alzahrani, Ahmed S. Al-Ghamdi, Mohammad S. Raafat, Bassem M. Alswat, Khaled A. Althobaiti, Yusuf S. Asiri, Yousif A. Int J Mol Sci Article Methamphetamine, a highly addictive central nervous system (CNS) stimulant, is used worldwide as an anorexiant and attention enhancer. Methamphetamine use during pregnancy, even at therapeutic doses, may harm fetal development. Here, we examined whether exposure to methamphetamine affects the morphogenesis and diversity of ventral midbrain dopaminergic neurons (VMDNs). The effects of methamphetamine on morphogenesis, viability, the release of mediator chemicals (such as ATP), and the expression of genes involved in neurogenesis were evaluated using VMDNs isolated from the embryos of timed-mated mice on embryonic day 12.5. We demonstrated that methamphetamine (10 µM; equivalent to its therapeutic dose) did not affect the viability and morphogenesis of VMDNs, but it reduced the ATP release negligibly. It significantly downregulated Lmx1a, En1, Pitx3, Th, Chl1, Dat, and Drd1 but did not affect Nurr1 or Bdnf expression. Our results illustrate that methamphetamine could impair VMDN differentiation by altering the expression of important neurogenesis-related genes. Overall, this study suggests that methamphetamine use may impair VMDNs in the fetus if taken during pregnancy. Therefore, it is essential to exercise strict caution for its use in expectant mothers. MDPI 2023-03-16 /pmc/articles/PMC10056332/ /pubmed/36982742 http://dx.doi.org/10.3390/ijms24065668 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Alsanie, Walaa F. Abdelrahman, Sherin Felimban, Raed I. Alkhatabi, Heba A. Gaber, Ahmed Alosimi, Ebtisam Abdulah Alhomrani, Majid Habeeballah, Hamza Hauser, Charlotte A. E. S. Alamri, Abdulhakeem Althobaiti, Aiysha Alsharif, Abdulaziz Alzahrani, Ahmed S. Al-Ghamdi, Mohammad S. Raafat, Bassem M. Alswat, Khaled A. Althobaiti, Yusuf S. Asiri, Yousif A. The Influence of Prenatal Exposure to Methamphetamine on the Development of Dopaminergic Neurons in the Ventral Midbrain |
title | The Influence of Prenatal Exposure to Methamphetamine on the Development of Dopaminergic Neurons in the Ventral Midbrain |
title_full | The Influence of Prenatal Exposure to Methamphetamine on the Development of Dopaminergic Neurons in the Ventral Midbrain |
title_fullStr | The Influence of Prenatal Exposure to Methamphetamine on the Development of Dopaminergic Neurons in the Ventral Midbrain |
title_full_unstemmed | The Influence of Prenatal Exposure to Methamphetamine on the Development of Dopaminergic Neurons in the Ventral Midbrain |
title_short | The Influence of Prenatal Exposure to Methamphetamine on the Development of Dopaminergic Neurons in the Ventral Midbrain |
title_sort | influence of prenatal exposure to methamphetamine on the development of dopaminergic neurons in the ventral midbrain |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10056332/ https://www.ncbi.nlm.nih.gov/pubmed/36982742 http://dx.doi.org/10.3390/ijms24065668 |
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