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Domoic Acid Oxidative Effects on the Microalgae Phaeodactylum tricornutum
Domoic acid (DA) is a natural occurring marine biotoxin. Oxidative stress generation due to DA exposure was reported in animals, but little is known on the phytoplankton community. The aim of this work was to verify whether exposure to DA in the marine diatom Phaeodactylum tricornutum favors reactiv...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10056487/ https://www.ncbi.nlm.nih.gov/pubmed/36983832 http://dx.doi.org/10.3390/life13030676 |
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author | Cabrera, Joaquin Puntarulo, Susana González, Paula Mariela |
author_facet | Cabrera, Joaquin Puntarulo, Susana González, Paula Mariela |
author_sort | Cabrera, Joaquin |
collection | PubMed |
description | Domoic acid (DA) is a natural occurring marine biotoxin. Oxidative stress generation due to DA exposure was reported in animals, but little is known on the phytoplankton community. The aim of this work was to verify whether exposure to DA in the marine diatom Phaeodactylum tricornutum favors reactive oxygen species (ROS) generation in the intracellular environment modifying its antioxidant capacity. Active species production, non-enzymatic antioxidant content, and antioxidant enzyme activities over the three growth phases of P. tricornutum exposed to 64 µM DA were evaluated. Results obtained in exponential growing cells showed a time-depending seven-fold increase in the 2′,7′ dichlorofluorescein diacetate dye oxidation rate. Superoxide dismutase and catalase activities showed a two-fold increase, and glutathione related enzymes activities were also significantly increased in treated diatoms as compared to controls. However, glutathione and ascorbate contents significantly decreased after incubation of the cells with DA. Similar effects were observed in latent and stationary phases of cell development. These results showed that DA could cause a severe oxidant-dependent impact on a non-toxic algae. |
format | Online Article Text |
id | pubmed-10056487 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100564872023-03-30 Domoic Acid Oxidative Effects on the Microalgae Phaeodactylum tricornutum Cabrera, Joaquin Puntarulo, Susana González, Paula Mariela Life (Basel) Article Domoic acid (DA) is a natural occurring marine biotoxin. Oxidative stress generation due to DA exposure was reported in animals, but little is known on the phytoplankton community. The aim of this work was to verify whether exposure to DA in the marine diatom Phaeodactylum tricornutum favors reactive oxygen species (ROS) generation in the intracellular environment modifying its antioxidant capacity. Active species production, non-enzymatic antioxidant content, and antioxidant enzyme activities over the three growth phases of P. tricornutum exposed to 64 µM DA were evaluated. Results obtained in exponential growing cells showed a time-depending seven-fold increase in the 2′,7′ dichlorofluorescein diacetate dye oxidation rate. Superoxide dismutase and catalase activities showed a two-fold increase, and glutathione related enzymes activities were also significantly increased in treated diatoms as compared to controls. However, glutathione and ascorbate contents significantly decreased after incubation of the cells with DA. Similar effects were observed in latent and stationary phases of cell development. These results showed that DA could cause a severe oxidant-dependent impact on a non-toxic algae. MDPI 2023-03-02 /pmc/articles/PMC10056487/ /pubmed/36983832 http://dx.doi.org/10.3390/life13030676 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cabrera, Joaquin Puntarulo, Susana González, Paula Mariela Domoic Acid Oxidative Effects on the Microalgae Phaeodactylum tricornutum |
title | Domoic Acid Oxidative Effects on the Microalgae Phaeodactylum tricornutum |
title_full | Domoic Acid Oxidative Effects on the Microalgae Phaeodactylum tricornutum |
title_fullStr | Domoic Acid Oxidative Effects on the Microalgae Phaeodactylum tricornutum |
title_full_unstemmed | Domoic Acid Oxidative Effects on the Microalgae Phaeodactylum tricornutum |
title_short | Domoic Acid Oxidative Effects on the Microalgae Phaeodactylum tricornutum |
title_sort | domoic acid oxidative effects on the microalgae phaeodactylum tricornutum |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10056487/ https://www.ncbi.nlm.nih.gov/pubmed/36983832 http://dx.doi.org/10.3390/life13030676 |
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