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The Role of Globularia alypum Explored Ex Vivo In Vitro on Human Colon Biopsies from Ulcerative Colitis Patients

The existing literature indicates that Globularia alypum L. (GA) influences inflammation and oxidative stress modulation in rats and in vitro. The present study aims to investigate the effects of this plant in patients with ulcerative colitis (UC) and normal controls. In our experiments, we pretreat...

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Autores principales: Hajji, Najla, Russo, Ilaria, Bianco, Jessica, Piazza, Ornella, Iovino, Paola, Santonicola, Antonella, Ciacci, Carolina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10056518/
https://www.ncbi.nlm.nih.gov/pubmed/36986188
http://dx.doi.org/10.3390/nu15061457
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author Hajji, Najla
Russo, Ilaria
Bianco, Jessica
Piazza, Ornella
Iovino, Paola
Santonicola, Antonella
Ciacci, Carolina
author_facet Hajji, Najla
Russo, Ilaria
Bianco, Jessica
Piazza, Ornella
Iovino, Paola
Santonicola, Antonella
Ciacci, Carolina
author_sort Hajji, Najla
collection PubMed
description The existing literature indicates that Globularia alypum L. (GA) influences inflammation and oxidative stress modulation in rats and in vitro. The present study aims to investigate the effects of this plant in patients with ulcerative colitis (UC) and normal controls. In our experiments, we pretreated colon biopsies from 46 UC patients and normal controls with GA leaves aqueous extract (GAAE) used at two concentrations (50 and 100 µg/mL) for 3 h, followed by Lipopolysaccharides (from Escherichia coli) stimulation. We analyzed the effects on inflammation by studying the cyclo-oxygenase-2, the intercellular adhesion molecule-1, the nuclear factor kappa B, and p38 mitogen-activated protein kinase expression. Moreover, we assessed the levels of interleukin 6, the superoxide dismutase activity, and nitric oxide release in the supernatant of cultures. Our data showed that GAAE influences UC patients and normal controls for most studied markers and enzymes. These results acknowledge, with some scientific evidence, the traditional belief in the anti-inflammatory properties of GA and represent the first demonstration of its effect in a human in vitro model of inflammatory conditions.
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spelling pubmed-100565182023-03-30 The Role of Globularia alypum Explored Ex Vivo In Vitro on Human Colon Biopsies from Ulcerative Colitis Patients Hajji, Najla Russo, Ilaria Bianco, Jessica Piazza, Ornella Iovino, Paola Santonicola, Antonella Ciacci, Carolina Nutrients Article The existing literature indicates that Globularia alypum L. (GA) influences inflammation and oxidative stress modulation in rats and in vitro. The present study aims to investigate the effects of this plant in patients with ulcerative colitis (UC) and normal controls. In our experiments, we pretreated colon biopsies from 46 UC patients and normal controls with GA leaves aqueous extract (GAAE) used at two concentrations (50 and 100 µg/mL) for 3 h, followed by Lipopolysaccharides (from Escherichia coli) stimulation. We analyzed the effects on inflammation by studying the cyclo-oxygenase-2, the intercellular adhesion molecule-1, the nuclear factor kappa B, and p38 mitogen-activated protein kinase expression. Moreover, we assessed the levels of interleukin 6, the superoxide dismutase activity, and nitric oxide release in the supernatant of cultures. Our data showed that GAAE influences UC patients and normal controls for most studied markers and enzymes. These results acknowledge, with some scientific evidence, the traditional belief in the anti-inflammatory properties of GA and represent the first demonstration of its effect in a human in vitro model of inflammatory conditions. MDPI 2023-03-17 /pmc/articles/PMC10056518/ /pubmed/36986188 http://dx.doi.org/10.3390/nu15061457 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hajji, Najla
Russo, Ilaria
Bianco, Jessica
Piazza, Ornella
Iovino, Paola
Santonicola, Antonella
Ciacci, Carolina
The Role of Globularia alypum Explored Ex Vivo In Vitro on Human Colon Biopsies from Ulcerative Colitis Patients
title The Role of Globularia alypum Explored Ex Vivo In Vitro on Human Colon Biopsies from Ulcerative Colitis Patients
title_full The Role of Globularia alypum Explored Ex Vivo In Vitro on Human Colon Biopsies from Ulcerative Colitis Patients
title_fullStr The Role of Globularia alypum Explored Ex Vivo In Vitro on Human Colon Biopsies from Ulcerative Colitis Patients
title_full_unstemmed The Role of Globularia alypum Explored Ex Vivo In Vitro on Human Colon Biopsies from Ulcerative Colitis Patients
title_short The Role of Globularia alypum Explored Ex Vivo In Vitro on Human Colon Biopsies from Ulcerative Colitis Patients
title_sort role of globularia alypum explored ex vivo in vitro on human colon biopsies from ulcerative colitis patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10056518/
https://www.ncbi.nlm.nih.gov/pubmed/36986188
http://dx.doi.org/10.3390/nu15061457
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