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Bone Healing of Critical-Sized Femoral Defects in Rats Treated with Erythropoietin Alone or in Combination with Xenograft

SIMPLE SUMMARY: The treatment of large bone defects caused by fractures is still a challenge in orthopaedics. In this study, the potential of erythropoietin to promote bone regeneration was investigated in an experimental rat model. Bone healing was monitored through objective radiological, osteoden...

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Autores principales: Vasileva, Radina, Chaprazov, Tzvetan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10056540/
https://www.ncbi.nlm.nih.gov/pubmed/36977235
http://dx.doi.org/10.3390/vetsci10030196
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author Vasileva, Radina
Chaprazov, Tzvetan
author_facet Vasileva, Radina
Chaprazov, Tzvetan
author_sort Vasileva, Radina
collection PubMed
description SIMPLE SUMMARY: The treatment of large bone defects caused by fractures is still a challenge in orthopaedics. In this study, the potential of erythropoietin to promote bone regeneration was investigated in an experimental rat model. Bone healing was monitored through objective radiological, osteodensitometric and histological methods. The assessment suggests that erythropoietin, applied locally on a collagen scaffold alone or in combination with a bone substitute, is capable of inducing bone healing in femoral defects. ABSTRACT: Critical-size bone defect models are the standard in studies of the osteogenic potential of biomaterials. The present investigation aimed to evaluate the ability of recombinant human erythropoietin (EPO) to induce trabecular bone healing either alone or combined with a xenograft in a rat femoral critical-size defect model. Five-mm bone defects were created in the femoral diaphysis of fifty-six skeletally mature male Wistar albino rats. The animals were divided into six groups: one control group and five experimental groups. The defects in the control group were left empty, whereas an absorbable collagen cone soaked either with saline or erythropoietin (alone or in combination with xenograft) was placed in locally treated groups. The systemic treatment group received EPO subcutaneously. Bone formation was objectively evaluated through radiography, osteodensitometry and histological examination on post-operative days 30 and 90. The results demonstrate that EPO, locally applied on a collagen scaffold, was capable of inducing bone healing, whereas the single systemically administered high EPO dose had only an insignificant effect on bone formation. The combination of EPO with a bone substitute under the form of cancellous granules resulted in more rapid integration between the xenograft and host bone.
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spelling pubmed-100565402023-03-30 Bone Healing of Critical-Sized Femoral Defects in Rats Treated with Erythropoietin Alone or in Combination with Xenograft Vasileva, Radina Chaprazov, Tzvetan Vet Sci Article SIMPLE SUMMARY: The treatment of large bone defects caused by fractures is still a challenge in orthopaedics. In this study, the potential of erythropoietin to promote bone regeneration was investigated in an experimental rat model. Bone healing was monitored through objective radiological, osteodensitometric and histological methods. The assessment suggests that erythropoietin, applied locally on a collagen scaffold alone or in combination with a bone substitute, is capable of inducing bone healing in femoral defects. ABSTRACT: Critical-size bone defect models are the standard in studies of the osteogenic potential of biomaterials. The present investigation aimed to evaluate the ability of recombinant human erythropoietin (EPO) to induce trabecular bone healing either alone or combined with a xenograft in a rat femoral critical-size defect model. Five-mm bone defects were created in the femoral diaphysis of fifty-six skeletally mature male Wistar albino rats. The animals were divided into six groups: one control group and five experimental groups. The defects in the control group were left empty, whereas an absorbable collagen cone soaked either with saline or erythropoietin (alone or in combination with xenograft) was placed in locally treated groups. The systemic treatment group received EPO subcutaneously. Bone formation was objectively evaluated through radiography, osteodensitometry and histological examination on post-operative days 30 and 90. The results demonstrate that EPO, locally applied on a collagen scaffold, was capable of inducing bone healing, whereas the single systemically administered high EPO dose had only an insignificant effect on bone formation. The combination of EPO with a bone substitute under the form of cancellous granules resulted in more rapid integration between the xenograft and host bone. MDPI 2023-03-05 /pmc/articles/PMC10056540/ /pubmed/36977235 http://dx.doi.org/10.3390/vetsci10030196 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vasileva, Radina
Chaprazov, Tzvetan
Bone Healing of Critical-Sized Femoral Defects in Rats Treated with Erythropoietin Alone or in Combination with Xenograft
title Bone Healing of Critical-Sized Femoral Defects in Rats Treated with Erythropoietin Alone or in Combination with Xenograft
title_full Bone Healing of Critical-Sized Femoral Defects in Rats Treated with Erythropoietin Alone or in Combination with Xenograft
title_fullStr Bone Healing of Critical-Sized Femoral Defects in Rats Treated with Erythropoietin Alone or in Combination with Xenograft
title_full_unstemmed Bone Healing of Critical-Sized Femoral Defects in Rats Treated with Erythropoietin Alone or in Combination with Xenograft
title_short Bone Healing of Critical-Sized Femoral Defects in Rats Treated with Erythropoietin Alone or in Combination with Xenograft
title_sort bone healing of critical-sized femoral defects in rats treated with erythropoietin alone or in combination with xenograft
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10056540/
https://www.ncbi.nlm.nih.gov/pubmed/36977235
http://dx.doi.org/10.3390/vetsci10030196
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