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Leucocyte Abnormalities in Synovial Fluid of Degenerative and Inflammatory Arthropathies

Genome damage has been related to the induction of autoimmune processes, chronic inflammation, and apoptosis. Recent studies suggest that some rheumatological diseases are associated with overall genomic instability in the T cell compartment. However, no data regarding leucocyte abnormalities in syn...

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Autores principales: Baggio, Chiara, Luisetto, Roberto, Boscaro, Carlotta, Scanu, Anna, Ramonda, Roberta, Albiero, Mattia, Sfriso, Paolo, Oliviero, Francesca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10056596/
https://www.ncbi.nlm.nih.gov/pubmed/36982526
http://dx.doi.org/10.3390/ijms24065450
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author Baggio, Chiara
Luisetto, Roberto
Boscaro, Carlotta
Scanu, Anna
Ramonda, Roberta
Albiero, Mattia
Sfriso, Paolo
Oliviero, Francesca
author_facet Baggio, Chiara
Luisetto, Roberto
Boscaro, Carlotta
Scanu, Anna
Ramonda, Roberta
Albiero, Mattia
Sfriso, Paolo
Oliviero, Francesca
author_sort Baggio, Chiara
collection PubMed
description Genome damage has been related to the induction of autoimmune processes, chronic inflammation, and apoptosis. Recent studies suggest that some rheumatological diseases are associated with overall genomic instability in the T cell compartment. However, no data regarding leucocyte abnormalities in synovial fluid (SF) and their relationship with inflammation are available. The aim of this study was to investigate cellular phenotypes in SF collected from patients with different inflammatory arthropathies, including rhematoid arthritis (RA), psoriatic arthritis (PsA), crystal-induced arthritis (CIA), and non-inflammatory arthropathies, such as osteoarthritis (OA). We found high percentage of micronuclei in SF from CIA compared to the other groups and a high frequency of pyknotic cell in RA and CIA patients. A correlation between pyknosis and immature polymorphonuclear cells with local inflammatory indices was observed. The study of the apoptosis process revealed an increased BAX expression in CIA and RA compared to OA and PsA, while Bcl-2 was higher in CIA. Caspase-3 activity was increased in SF from RA patients and correlates with inflammatory and anti-inflammatory cytokines. In conclusion, our results showed that inflammatory SF is associated with genomic instability and abnormal cell subsets.
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spelling pubmed-100565962023-03-30 Leucocyte Abnormalities in Synovial Fluid of Degenerative and Inflammatory Arthropathies Baggio, Chiara Luisetto, Roberto Boscaro, Carlotta Scanu, Anna Ramonda, Roberta Albiero, Mattia Sfriso, Paolo Oliviero, Francesca Int J Mol Sci Article Genome damage has been related to the induction of autoimmune processes, chronic inflammation, and apoptosis. Recent studies suggest that some rheumatological diseases are associated with overall genomic instability in the T cell compartment. However, no data regarding leucocyte abnormalities in synovial fluid (SF) and their relationship with inflammation are available. The aim of this study was to investigate cellular phenotypes in SF collected from patients with different inflammatory arthropathies, including rhematoid arthritis (RA), psoriatic arthritis (PsA), crystal-induced arthritis (CIA), and non-inflammatory arthropathies, such as osteoarthritis (OA). We found high percentage of micronuclei in SF from CIA compared to the other groups and a high frequency of pyknotic cell in RA and CIA patients. A correlation between pyknosis and immature polymorphonuclear cells with local inflammatory indices was observed. The study of the apoptosis process revealed an increased BAX expression in CIA and RA compared to OA and PsA, while Bcl-2 was higher in CIA. Caspase-3 activity was increased in SF from RA patients and correlates with inflammatory and anti-inflammatory cytokines. In conclusion, our results showed that inflammatory SF is associated with genomic instability and abnormal cell subsets. MDPI 2023-03-13 /pmc/articles/PMC10056596/ /pubmed/36982526 http://dx.doi.org/10.3390/ijms24065450 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Baggio, Chiara
Luisetto, Roberto
Boscaro, Carlotta
Scanu, Anna
Ramonda, Roberta
Albiero, Mattia
Sfriso, Paolo
Oliviero, Francesca
Leucocyte Abnormalities in Synovial Fluid of Degenerative and Inflammatory Arthropathies
title Leucocyte Abnormalities in Synovial Fluid of Degenerative and Inflammatory Arthropathies
title_full Leucocyte Abnormalities in Synovial Fluid of Degenerative and Inflammatory Arthropathies
title_fullStr Leucocyte Abnormalities in Synovial Fluid of Degenerative and Inflammatory Arthropathies
title_full_unstemmed Leucocyte Abnormalities in Synovial Fluid of Degenerative and Inflammatory Arthropathies
title_short Leucocyte Abnormalities in Synovial Fluid of Degenerative and Inflammatory Arthropathies
title_sort leucocyte abnormalities in synovial fluid of degenerative and inflammatory arthropathies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10056596/
https://www.ncbi.nlm.nih.gov/pubmed/36982526
http://dx.doi.org/10.3390/ijms24065450
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