Cargando…

The Mechanism of Kelulut Honey in Reversing Metabolic Changes in Rats Fed with High-Carbohydrate High-Fat Diet

Metabolic syndrome (MetS) is composed of central obesity, hyperglycemia, dyslipidemia and hypertension that increase an individual’s tendency to develop type 2 diabetes mellitus and cardiovascular diseases. Kelulut honey (KH) produced by stingless bee species has a rich phenolic profile. Recent stud...

Descripción completa

Detalles Bibliográficos
Autores principales: Hashim, Khairun-Nisa, Chin, Kok-Yong, Ahmad, Fairus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10056699/
https://www.ncbi.nlm.nih.gov/pubmed/36985762
http://dx.doi.org/10.3390/molecules28062790
_version_ 1785016187842723840
author Hashim, Khairun-Nisa
Chin, Kok-Yong
Ahmad, Fairus
author_facet Hashim, Khairun-Nisa
Chin, Kok-Yong
Ahmad, Fairus
author_sort Hashim, Khairun-Nisa
collection PubMed
description Metabolic syndrome (MetS) is composed of central obesity, hyperglycemia, dyslipidemia and hypertension that increase an individual’s tendency to develop type 2 diabetes mellitus and cardiovascular diseases. Kelulut honey (KH) produced by stingless bee species has a rich phenolic profile. Recent studies have demonstrated that KH could suppress components of MetS, but its mechanisms of action are unknown. A total of 18 male Wistar rats were randomly divided into control rats (C group) (n = 6), MetS rats fed with a high carbohydrate high fat (HCHF) diet (HCHF group) (n = 6), and MetS rats fed with HCHF diet and treated with KH (HCHF + KH group) (n = 6). The HCHF + KH group received 1.0 g/kg/day KH via oral gavage from week 9 to 16 after HCHF diet initiation. Compared to the C group, the MetS group experienced a significant increase in body weight, body mass index, systolic (SBP) and diastolic blood pressure (DBP), serum triglyceride (TG) and leptin, as well as the area and perimeter of adipocyte cells at the end of the study. The MetS group also experienced a significant decrease in serum HDL levels versus the C group. KH supplementation reversed the changes in serum TG, HDL, leptin, adiponectin and corticosterone levels, SBP, DBP, as well as adipose tissue 11β-hydroxysteroid dehydrogenase type 1 (11βHSD1) level, area and perimeter at the end of the study. In addition, histological observations also showed that KH administration reduced fat deposition within hepatocytes, and prevented deterioration of pancreatic islet and renal glomerulus. In conclusion, KH is effective in preventing MetS by suppressing leptin, corticosterone and 11βHSD1 levels while elevating adiponectin levels.
format Online
Article
Text
id pubmed-10056699
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-100566992023-03-30 The Mechanism of Kelulut Honey in Reversing Metabolic Changes in Rats Fed with High-Carbohydrate High-Fat Diet Hashim, Khairun-Nisa Chin, Kok-Yong Ahmad, Fairus Molecules Article Metabolic syndrome (MetS) is composed of central obesity, hyperglycemia, dyslipidemia and hypertension that increase an individual’s tendency to develop type 2 diabetes mellitus and cardiovascular diseases. Kelulut honey (KH) produced by stingless bee species has a rich phenolic profile. Recent studies have demonstrated that KH could suppress components of MetS, but its mechanisms of action are unknown. A total of 18 male Wistar rats were randomly divided into control rats (C group) (n = 6), MetS rats fed with a high carbohydrate high fat (HCHF) diet (HCHF group) (n = 6), and MetS rats fed with HCHF diet and treated with KH (HCHF + KH group) (n = 6). The HCHF + KH group received 1.0 g/kg/day KH via oral gavage from week 9 to 16 after HCHF diet initiation. Compared to the C group, the MetS group experienced a significant increase in body weight, body mass index, systolic (SBP) and diastolic blood pressure (DBP), serum triglyceride (TG) and leptin, as well as the area and perimeter of adipocyte cells at the end of the study. The MetS group also experienced a significant decrease in serum HDL levels versus the C group. KH supplementation reversed the changes in serum TG, HDL, leptin, adiponectin and corticosterone levels, SBP, DBP, as well as adipose tissue 11β-hydroxysteroid dehydrogenase type 1 (11βHSD1) level, area and perimeter at the end of the study. In addition, histological observations also showed that KH administration reduced fat deposition within hepatocytes, and prevented deterioration of pancreatic islet and renal glomerulus. In conclusion, KH is effective in preventing MetS by suppressing leptin, corticosterone and 11βHSD1 levels while elevating adiponectin levels. MDPI 2023-03-20 /pmc/articles/PMC10056699/ /pubmed/36985762 http://dx.doi.org/10.3390/molecules28062790 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hashim, Khairun-Nisa
Chin, Kok-Yong
Ahmad, Fairus
The Mechanism of Kelulut Honey in Reversing Metabolic Changes in Rats Fed with High-Carbohydrate High-Fat Diet
title The Mechanism of Kelulut Honey in Reversing Metabolic Changes in Rats Fed with High-Carbohydrate High-Fat Diet
title_full The Mechanism of Kelulut Honey in Reversing Metabolic Changes in Rats Fed with High-Carbohydrate High-Fat Diet
title_fullStr The Mechanism of Kelulut Honey in Reversing Metabolic Changes in Rats Fed with High-Carbohydrate High-Fat Diet
title_full_unstemmed The Mechanism of Kelulut Honey in Reversing Metabolic Changes in Rats Fed with High-Carbohydrate High-Fat Diet
title_short The Mechanism of Kelulut Honey in Reversing Metabolic Changes in Rats Fed with High-Carbohydrate High-Fat Diet
title_sort mechanism of kelulut honey in reversing metabolic changes in rats fed with high-carbohydrate high-fat diet
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10056699/
https://www.ncbi.nlm.nih.gov/pubmed/36985762
http://dx.doi.org/10.3390/molecules28062790
work_keys_str_mv AT hashimkhairunnisa themechanismofkeluluthoneyinreversingmetabolicchangesinratsfedwithhighcarbohydratehighfatdiet
AT chinkokyong themechanismofkeluluthoneyinreversingmetabolicchangesinratsfedwithhighcarbohydratehighfatdiet
AT ahmadfairus themechanismofkeluluthoneyinreversingmetabolicchangesinratsfedwithhighcarbohydratehighfatdiet
AT hashimkhairunnisa mechanismofkeluluthoneyinreversingmetabolicchangesinratsfedwithhighcarbohydratehighfatdiet
AT chinkokyong mechanismofkeluluthoneyinreversingmetabolicchangesinratsfedwithhighcarbohydratehighfatdiet
AT ahmadfairus mechanismofkeluluthoneyinreversingmetabolicchangesinratsfedwithhighcarbohydratehighfatdiet