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Nailfold Videocapillaroscopy for Non-Invasive Assessment of Microcirculation and Prognostic Correlation with Endothelial Dysfunction, Cardiovascular Risk Factors, and Non-HLA Antibodies in Heart Transplant Recipients: A Pilot Study

Early identification of allograft vasculopathy and the concomitant elimination of adverse risk factors is essential for improving the long-term prognosis of heart transplant (HTx) recipients with underlying cardiovascular disease (CVD). The major aim of this pilot study was to conduct a non-invasive...

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Autores principales: Sikorska, Dorota, Kamińska, Dorota, Catar, Rusan, Wu, Dashan, Zhao, Hongfan, Wang, Pinchao, Kamhieh-Milz, Julian, Banasik, Mirosław, Kusztal, Mariusz, Cielecka, Magdalena, Zakliczyński, Michał, Rutkowski, Rafał, Korybalska, Katarzyna, Heidecke, Harald, Moll, Guido, Samborski, Włodzimierz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10056970/
https://www.ncbi.nlm.nih.gov/pubmed/36983302
http://dx.doi.org/10.3390/jcm12062302
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author Sikorska, Dorota
Kamińska, Dorota
Catar, Rusan
Wu, Dashan
Zhao, Hongfan
Wang, Pinchao
Kamhieh-Milz, Julian
Banasik, Mirosław
Kusztal, Mariusz
Cielecka, Magdalena
Zakliczyński, Michał
Rutkowski, Rafał
Korybalska, Katarzyna
Heidecke, Harald
Moll, Guido
Samborski, Włodzimierz
author_facet Sikorska, Dorota
Kamińska, Dorota
Catar, Rusan
Wu, Dashan
Zhao, Hongfan
Wang, Pinchao
Kamhieh-Milz, Julian
Banasik, Mirosław
Kusztal, Mariusz
Cielecka, Magdalena
Zakliczyński, Michał
Rutkowski, Rafał
Korybalska, Katarzyna
Heidecke, Harald
Moll, Guido
Samborski, Włodzimierz
author_sort Sikorska, Dorota
collection PubMed
description Early identification of allograft vasculopathy and the concomitant elimination of adverse risk factors is essential for improving the long-term prognosis of heart transplant (HTx) recipients with underlying cardiovascular disease (CVD). The major aim of this pilot study was to conduct a non-invasive imaging evaluation of the HTx patient microcirculation by employing nailfold video-capillaroscopy (NVC) in a well-characterized patient and control cohort, and to correlate these data with endothelial cell function, accompanied by studies of traditional cardiovascular risk factors and non-HLA antibodies in HTx recipients. Ten patients undergoing HTx (mean age of 38 ± 14 years) were recruited for the study and compared to a control group of 12 well-matched healthy volunteers (mean age 35 ± 5 years) with normal body mass index (BMI). Detailed medical records were collected from all individuals. NVC was performed using CapillaryScope 200 MEDL4N microscope. For functional readout and correlation analysis, endothelial cell network formation in conjunction with measurements of patient serum levels of vascular endothelial growth factor (VEGF) and non-HLA autoantibodies directed against the angiotensin II type-1-receptor (anti-AT1R-Ab), endothelin-1 type-A-receptor (anti-ETAR-Ab), protease-activated receptor-1 (anti-PAR-1-Ab), and VEGF-A (anti-VEGF-A-Ab) were studied. Our NVC analysis found that the average apical loop diameter of nailfold capillaries was significantly increased in HTx recipients (p = 0.001). In addition, HTx patients with more prominent changes in capillaroscopic patterns were characterized by the presence of traditional cardiovascular risk factors, and HTx patients had increased levels of anti-AT1R-ab, anti-ETAR-ab, and anti-VEGF-A-Ab (p = 0.017, p = 0.025, and p = 0.003, respectively). Capillary diameters most strongly correlated with elevated serum levels of troponin T and triglycerides (R = 0.69, p = 0.028 and R = 0.81, p = 0.004, respectively). In conclusion, we found that an abnormal NVC pattern in HTx patients is associated with traditional CVD risk factors and that NVC is a useful non-invasive tool to conveniently monitor changes in the microvasculature of HTx patients.
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spelling pubmed-100569702023-03-30 Nailfold Videocapillaroscopy for Non-Invasive Assessment of Microcirculation and Prognostic Correlation with Endothelial Dysfunction, Cardiovascular Risk Factors, and Non-HLA Antibodies in Heart Transplant Recipients: A Pilot Study Sikorska, Dorota Kamińska, Dorota Catar, Rusan Wu, Dashan Zhao, Hongfan Wang, Pinchao Kamhieh-Milz, Julian Banasik, Mirosław Kusztal, Mariusz Cielecka, Magdalena Zakliczyński, Michał Rutkowski, Rafał Korybalska, Katarzyna Heidecke, Harald Moll, Guido Samborski, Włodzimierz J Clin Med Article Early identification of allograft vasculopathy and the concomitant elimination of adverse risk factors is essential for improving the long-term prognosis of heart transplant (HTx) recipients with underlying cardiovascular disease (CVD). The major aim of this pilot study was to conduct a non-invasive imaging evaluation of the HTx patient microcirculation by employing nailfold video-capillaroscopy (NVC) in a well-characterized patient and control cohort, and to correlate these data with endothelial cell function, accompanied by studies of traditional cardiovascular risk factors and non-HLA antibodies in HTx recipients. Ten patients undergoing HTx (mean age of 38 ± 14 years) were recruited for the study and compared to a control group of 12 well-matched healthy volunteers (mean age 35 ± 5 years) with normal body mass index (BMI). Detailed medical records were collected from all individuals. NVC was performed using CapillaryScope 200 MEDL4N microscope. For functional readout and correlation analysis, endothelial cell network formation in conjunction with measurements of patient serum levels of vascular endothelial growth factor (VEGF) and non-HLA autoantibodies directed against the angiotensin II type-1-receptor (anti-AT1R-Ab), endothelin-1 type-A-receptor (anti-ETAR-Ab), protease-activated receptor-1 (anti-PAR-1-Ab), and VEGF-A (anti-VEGF-A-Ab) were studied. Our NVC analysis found that the average apical loop diameter of nailfold capillaries was significantly increased in HTx recipients (p = 0.001). In addition, HTx patients with more prominent changes in capillaroscopic patterns were characterized by the presence of traditional cardiovascular risk factors, and HTx patients had increased levels of anti-AT1R-ab, anti-ETAR-ab, and anti-VEGF-A-Ab (p = 0.017, p = 0.025, and p = 0.003, respectively). Capillary diameters most strongly correlated with elevated serum levels of troponin T and triglycerides (R = 0.69, p = 0.028 and R = 0.81, p = 0.004, respectively). In conclusion, we found that an abnormal NVC pattern in HTx patients is associated with traditional CVD risk factors and that NVC is a useful non-invasive tool to conveniently monitor changes in the microvasculature of HTx patients. MDPI 2023-03-16 /pmc/articles/PMC10056970/ /pubmed/36983302 http://dx.doi.org/10.3390/jcm12062302 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sikorska, Dorota
Kamińska, Dorota
Catar, Rusan
Wu, Dashan
Zhao, Hongfan
Wang, Pinchao
Kamhieh-Milz, Julian
Banasik, Mirosław
Kusztal, Mariusz
Cielecka, Magdalena
Zakliczyński, Michał
Rutkowski, Rafał
Korybalska, Katarzyna
Heidecke, Harald
Moll, Guido
Samborski, Włodzimierz
Nailfold Videocapillaroscopy for Non-Invasive Assessment of Microcirculation and Prognostic Correlation with Endothelial Dysfunction, Cardiovascular Risk Factors, and Non-HLA Antibodies in Heart Transplant Recipients: A Pilot Study
title Nailfold Videocapillaroscopy for Non-Invasive Assessment of Microcirculation and Prognostic Correlation with Endothelial Dysfunction, Cardiovascular Risk Factors, and Non-HLA Antibodies in Heart Transplant Recipients: A Pilot Study
title_full Nailfold Videocapillaroscopy for Non-Invasive Assessment of Microcirculation and Prognostic Correlation with Endothelial Dysfunction, Cardiovascular Risk Factors, and Non-HLA Antibodies in Heart Transplant Recipients: A Pilot Study
title_fullStr Nailfold Videocapillaroscopy for Non-Invasive Assessment of Microcirculation and Prognostic Correlation with Endothelial Dysfunction, Cardiovascular Risk Factors, and Non-HLA Antibodies in Heart Transplant Recipients: A Pilot Study
title_full_unstemmed Nailfold Videocapillaroscopy for Non-Invasive Assessment of Microcirculation and Prognostic Correlation with Endothelial Dysfunction, Cardiovascular Risk Factors, and Non-HLA Antibodies in Heart Transplant Recipients: A Pilot Study
title_short Nailfold Videocapillaroscopy for Non-Invasive Assessment of Microcirculation and Prognostic Correlation with Endothelial Dysfunction, Cardiovascular Risk Factors, and Non-HLA Antibodies in Heart Transplant Recipients: A Pilot Study
title_sort nailfold videocapillaroscopy for non-invasive assessment of microcirculation and prognostic correlation with endothelial dysfunction, cardiovascular risk factors, and non-hla antibodies in heart transplant recipients: a pilot study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10056970/
https://www.ncbi.nlm.nih.gov/pubmed/36983302
http://dx.doi.org/10.3390/jcm12062302
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