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Downregulation of DROSHA: Could It Affect miRNA Biogenesis in Endometriotic Menstrual Blood Mesenchymal Stem Cells?

Menstrual blood mesenchymal stem cells (MenSCs) have gained prominence in the endometriosis scientific community, given their multifunctional roles in regenerative medicine as a noninvasive source for future clinical applications. In addition, changes in post-transcriptional regulation via miRNAs ha...

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Autores principales: Cressoni, Ana Clara Lagazzi, Penariol, Letícia B. C., Padovan, Cristiana Carolina, Orellana, Maristela D., Rosa-e-Silva, Júlio Cesar, Poli-Neto, Omero Benedicto, Ferriani, Rui Alberto, de Paz, Cláudia Cristina Paro, Meola, Juliana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10057010/
https://www.ncbi.nlm.nih.gov/pubmed/36983035
http://dx.doi.org/10.3390/ijms24065963
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author Cressoni, Ana Clara Lagazzi
Penariol, Letícia B. C.
Padovan, Cristiana Carolina
Orellana, Maristela D.
Rosa-e-Silva, Júlio Cesar
Poli-Neto, Omero Benedicto
Ferriani, Rui Alberto
de Paz, Cláudia Cristina Paro
Meola, Juliana
author_facet Cressoni, Ana Clara Lagazzi
Penariol, Letícia B. C.
Padovan, Cristiana Carolina
Orellana, Maristela D.
Rosa-e-Silva, Júlio Cesar
Poli-Neto, Omero Benedicto
Ferriani, Rui Alberto
de Paz, Cláudia Cristina Paro
Meola, Juliana
author_sort Cressoni, Ana Clara Lagazzi
collection PubMed
description Menstrual blood mesenchymal stem cells (MenSCs) have gained prominence in the endometriosis scientific community, given their multifunctional roles in regenerative medicine as a noninvasive source for future clinical applications. In addition, changes in post-transcriptional regulation via miRNAs have been explored in endometriotic MenSCs with a role in modulating proliferation, angiogenesis, differentiation, stemness, self-renewal, and the mesenchymal–epithelial transition process. In this sense, homeostasis of the miRNA biosynthesis pathway is essential for several cellular processes and is related to the self-renewal and differentiation of progenitor cells. However, no studies have investigated the miRNA biogenesis pathway in endometriotic MenSCs. In this study, we profiled the expression of eight central genes for the miRNA biosynthesis pathway under experimental conditions involving a two-dimensional culture of MenSCs obtained from healthy women (n = 10) and women with endometriosis (n = 10) using RT-qPCR and reported a two-fold decrease in DROSHA expression in the disease. In addition, miR-128-3p, miR-27a-3p, miR-27b-3p, miR-181a-5p, miR-181b-5p, miR-452-3p, miR-216a-5p, miR-216b-5p, and miR-93-5p, which have been associated with endometriosis, were identified through in silico analyses as negative regulators of DROSHA. Because DROSHA is essential for miRNA maturation, our findings may justify the identification of different profiles of miRNAs with DROSHA-dependent biogenesis in endometriosis.
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spelling pubmed-100570102023-03-30 Downregulation of DROSHA: Could It Affect miRNA Biogenesis in Endometriotic Menstrual Blood Mesenchymal Stem Cells? Cressoni, Ana Clara Lagazzi Penariol, Letícia B. C. Padovan, Cristiana Carolina Orellana, Maristela D. Rosa-e-Silva, Júlio Cesar Poli-Neto, Omero Benedicto Ferriani, Rui Alberto de Paz, Cláudia Cristina Paro Meola, Juliana Int J Mol Sci Article Menstrual blood mesenchymal stem cells (MenSCs) have gained prominence in the endometriosis scientific community, given their multifunctional roles in regenerative medicine as a noninvasive source for future clinical applications. In addition, changes in post-transcriptional regulation via miRNAs have been explored in endometriotic MenSCs with a role in modulating proliferation, angiogenesis, differentiation, stemness, self-renewal, and the mesenchymal–epithelial transition process. In this sense, homeostasis of the miRNA biosynthesis pathway is essential for several cellular processes and is related to the self-renewal and differentiation of progenitor cells. However, no studies have investigated the miRNA biogenesis pathway in endometriotic MenSCs. In this study, we profiled the expression of eight central genes for the miRNA biosynthesis pathway under experimental conditions involving a two-dimensional culture of MenSCs obtained from healthy women (n = 10) and women with endometriosis (n = 10) using RT-qPCR and reported a two-fold decrease in DROSHA expression in the disease. In addition, miR-128-3p, miR-27a-3p, miR-27b-3p, miR-181a-5p, miR-181b-5p, miR-452-3p, miR-216a-5p, miR-216b-5p, and miR-93-5p, which have been associated with endometriosis, were identified through in silico analyses as negative regulators of DROSHA. Because DROSHA is essential for miRNA maturation, our findings may justify the identification of different profiles of miRNAs with DROSHA-dependent biogenesis in endometriosis. MDPI 2023-03-22 /pmc/articles/PMC10057010/ /pubmed/36983035 http://dx.doi.org/10.3390/ijms24065963 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cressoni, Ana Clara Lagazzi
Penariol, Letícia B. C.
Padovan, Cristiana Carolina
Orellana, Maristela D.
Rosa-e-Silva, Júlio Cesar
Poli-Neto, Omero Benedicto
Ferriani, Rui Alberto
de Paz, Cláudia Cristina Paro
Meola, Juliana
Downregulation of DROSHA: Could It Affect miRNA Biogenesis in Endometriotic Menstrual Blood Mesenchymal Stem Cells?
title Downregulation of DROSHA: Could It Affect miRNA Biogenesis in Endometriotic Menstrual Blood Mesenchymal Stem Cells?
title_full Downregulation of DROSHA: Could It Affect miRNA Biogenesis in Endometriotic Menstrual Blood Mesenchymal Stem Cells?
title_fullStr Downregulation of DROSHA: Could It Affect miRNA Biogenesis in Endometriotic Menstrual Blood Mesenchymal Stem Cells?
title_full_unstemmed Downregulation of DROSHA: Could It Affect miRNA Biogenesis in Endometriotic Menstrual Blood Mesenchymal Stem Cells?
title_short Downregulation of DROSHA: Could It Affect miRNA Biogenesis in Endometriotic Menstrual Blood Mesenchymal Stem Cells?
title_sort downregulation of drosha: could it affect mirna biogenesis in endometriotic menstrual blood mesenchymal stem cells?
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10057010/
https://www.ncbi.nlm.nih.gov/pubmed/36983035
http://dx.doi.org/10.3390/ijms24065963
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