Mechanochemical Synergism of Reactive Oxygen Species Influences on RBC Membrane

The influences of various factors on blood lead to the formation of extra reactive oxygen species (ROS), resulting in the disruption of morphology and functions of red blood cells (RBCs). This study considers the mechanisms of the mechanochemical synergism of [Formula: see text] free radicals, which are most active in the initiation of lipid peroxidation (LPO) in RBC membranes, and [Formula: see text] molecules, the largest typical diffusion path. Using kinetic models of differential equations describing [Formula: see text] and [Formula: see text] , we discuss two levels of mechanochemical synergism that occur simultaneously: (1) synergism that ensures the delivery of highly active free radicals [Formula: see text] to RBC membranes and (2) a positive feedback system between [Formula: see text] and [Formula: see text] , resulting in the partial restoration of spent molecules. As a result of these ROS synergisms, the efficiency of LPO in RBC membranes sharply increases. In blood, the appearance of [Formula: see text] free radicals is due to the interaction of [Formula: see text] molecules with free iron ions ([Formula: see text]) which arise as a result of heme degradation. We experimentally established the quantitative dependences of [Formula: see text] using the methods of spectrophotometry and nonlinear curve fitting. This study extends the analysis of the influence of ROS mechanisms in RBC suspensions.