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Mechanochemical Synergism of Reactive Oxygen Species Influences on RBC Membrane

The influences of various factors on blood lead to the formation of extra reactive oxygen species (ROS), resulting in the disruption of morphology and functions of red blood cells (RBCs). This study considers the mechanisms of the mechanochemical synergism of [Formula: see text] free radicals, which...

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Autores principales: Kozlova, Elena, Sergunova, Viktoria, Sherstyukova, Ekaterina, Grechko, Andrey, Lyapunova, Snezhanna, Inozemtsev, Vladimir, Kozlov, Aleksandr, Gudkova, Olga, Chernysh, Aleksandr
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10057059/
https://www.ncbi.nlm.nih.gov/pubmed/36983026
http://dx.doi.org/10.3390/ijms24065952
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author Kozlova, Elena
Sergunova, Viktoria
Sherstyukova, Ekaterina
Grechko, Andrey
Lyapunova, Snezhanna
Inozemtsev, Vladimir
Kozlov, Aleksandr
Gudkova, Olga
Chernysh, Aleksandr
author_facet Kozlova, Elena
Sergunova, Viktoria
Sherstyukova, Ekaterina
Grechko, Andrey
Lyapunova, Snezhanna
Inozemtsev, Vladimir
Kozlov, Aleksandr
Gudkova, Olga
Chernysh, Aleksandr
author_sort Kozlova, Elena
collection PubMed
description The influences of various factors on blood lead to the formation of extra reactive oxygen species (ROS), resulting in the disruption of morphology and functions of red blood cells (RBCs). This study considers the mechanisms of the mechanochemical synergism of [Formula: see text] free radicals, which are most active in the initiation of lipid peroxidation (LPO) in RBC membranes, and [Formula: see text] molecules, the largest typical diffusion path. Using kinetic models of differential equations describing [Formula: see text] and [Formula: see text] , we discuss two levels of mechanochemical synergism that occur simultaneously: (1) synergism that ensures the delivery of highly active free radicals [Formula: see text] to RBC membranes and (2) a positive feedback system between [Formula: see text] and [Formula: see text] , resulting in the partial restoration of spent molecules. As a result of these ROS synergisms, the efficiency of LPO in RBC membranes sharply increases. In blood, the appearance of [Formula: see text] free radicals is due to the interaction of [Formula: see text] molecules with free iron ions ([Formula: see text]) which arise as a result of heme degradation. We experimentally established the quantitative dependences of [Formula: see text] using the methods of spectrophotometry and nonlinear curve fitting. This study extends the analysis of the influence of ROS mechanisms in RBC suspensions.
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spelling pubmed-100570592023-03-30 Mechanochemical Synergism of Reactive Oxygen Species Influences on RBC Membrane Kozlova, Elena Sergunova, Viktoria Sherstyukova, Ekaterina Grechko, Andrey Lyapunova, Snezhanna Inozemtsev, Vladimir Kozlov, Aleksandr Gudkova, Olga Chernysh, Aleksandr Int J Mol Sci Article The influences of various factors on blood lead to the formation of extra reactive oxygen species (ROS), resulting in the disruption of morphology and functions of red blood cells (RBCs). This study considers the mechanisms of the mechanochemical synergism of [Formula: see text] free radicals, which are most active in the initiation of lipid peroxidation (LPO) in RBC membranes, and [Formula: see text] molecules, the largest typical diffusion path. Using kinetic models of differential equations describing [Formula: see text] and [Formula: see text] , we discuss two levels of mechanochemical synergism that occur simultaneously: (1) synergism that ensures the delivery of highly active free radicals [Formula: see text] to RBC membranes and (2) a positive feedback system between [Formula: see text] and [Formula: see text] , resulting in the partial restoration of spent molecules. As a result of these ROS synergisms, the efficiency of LPO in RBC membranes sharply increases. In blood, the appearance of [Formula: see text] free radicals is due to the interaction of [Formula: see text] molecules with free iron ions ([Formula: see text]) which arise as a result of heme degradation. We experimentally established the quantitative dependences of [Formula: see text] using the methods of spectrophotometry and nonlinear curve fitting. This study extends the analysis of the influence of ROS mechanisms in RBC suspensions. MDPI 2023-03-21 /pmc/articles/PMC10057059/ /pubmed/36983026 http://dx.doi.org/10.3390/ijms24065952 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kozlova, Elena
Sergunova, Viktoria
Sherstyukova, Ekaterina
Grechko, Andrey
Lyapunova, Snezhanna
Inozemtsev, Vladimir
Kozlov, Aleksandr
Gudkova, Olga
Chernysh, Aleksandr
Mechanochemical Synergism of Reactive Oxygen Species Influences on RBC Membrane
title Mechanochemical Synergism of Reactive Oxygen Species Influences on RBC Membrane
title_full Mechanochemical Synergism of Reactive Oxygen Species Influences on RBC Membrane
title_fullStr Mechanochemical Synergism of Reactive Oxygen Species Influences on RBC Membrane
title_full_unstemmed Mechanochemical Synergism of Reactive Oxygen Species Influences on RBC Membrane
title_short Mechanochemical Synergism of Reactive Oxygen Species Influences on RBC Membrane
title_sort mechanochemical synergism of reactive oxygen species influences on rbc membrane
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10057059/
https://www.ncbi.nlm.nih.gov/pubmed/36983026
http://dx.doi.org/10.3390/ijms24065952
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