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Photoacoustic Flow Cytometry Using Functionalized Microspheres for Selective Detection of Bacteria

Photoacoustic flow cytometry is a method to detect rare analytes in fluids. We developed photoacoustic flow cytometry to detect pathological cells in body fluids, such as circulating tumor cells or bacteria in blood. In order to induce specific optical absorption in bacteria, we use modified bacteri...

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Detalles Bibliográficos
Autores principales: Edgar, Robert H., Samson, Anie-Pier, Kocsis, Tori, Viator, John A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10057399/
https://www.ncbi.nlm.nih.gov/pubmed/36984980
http://dx.doi.org/10.3390/mi14030573
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author Edgar, Robert H.
Samson, Anie-Pier
Kocsis, Tori
Viator, John A.
author_facet Edgar, Robert H.
Samson, Anie-Pier
Kocsis, Tori
Viator, John A.
author_sort Edgar, Robert H.
collection PubMed
description Photoacoustic flow cytometry is a method to detect rare analytes in fluids. We developed photoacoustic flow cytometry to detect pathological cells in body fluids, such as circulating tumor cells or bacteria in blood. In order to induce specific optical absorption in bacteria, we use modified bacteriophage that precisely target bacterial species or subspecies for rapid identification. In order to reduce detection variability and to halt the lytic lifescycle that results in lysis of the bacteria, we attached dyed latex microspheres to the tail fibers of bacteriophage that retained the bacterial recognition binding sites. We tested these microsphere complexes using Salmonella enterica (Salmonella) and Escherichia coli (E. coli) bacteria and found robust and specific detection of targeted bacteria. In our work we used LT2, a strain of Salmonella, against K12, a strain of E. coli. Using Det7, a bacteriophage that binds to LT2 and not to K12, we detected an average of [Formula: see text] of LT2 versus [Formula: see text] of K12 using red microspheres and [Formula: see text] of LT2 versus [Formula: see text] of K12 using blue microspheres. These results confirmed our ability to selectively detect bacterial species using photoacoustic flow cytometry.
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spelling pubmed-100573992023-03-30 Photoacoustic Flow Cytometry Using Functionalized Microspheres for Selective Detection of Bacteria Edgar, Robert H. Samson, Anie-Pier Kocsis, Tori Viator, John A. Micromachines (Basel) Article Photoacoustic flow cytometry is a method to detect rare analytes in fluids. We developed photoacoustic flow cytometry to detect pathological cells in body fluids, such as circulating tumor cells or bacteria in blood. In order to induce specific optical absorption in bacteria, we use modified bacteriophage that precisely target bacterial species or subspecies for rapid identification. In order to reduce detection variability and to halt the lytic lifescycle that results in lysis of the bacteria, we attached dyed latex microspheres to the tail fibers of bacteriophage that retained the bacterial recognition binding sites. We tested these microsphere complexes using Salmonella enterica (Salmonella) and Escherichia coli (E. coli) bacteria and found robust and specific detection of targeted bacteria. In our work we used LT2, a strain of Salmonella, against K12, a strain of E. coli. Using Det7, a bacteriophage that binds to LT2 and not to K12, we detected an average of [Formula: see text] of LT2 versus [Formula: see text] of K12 using red microspheres and [Formula: see text] of LT2 versus [Formula: see text] of K12 using blue microspheres. These results confirmed our ability to selectively detect bacterial species using photoacoustic flow cytometry. MDPI 2023-02-28 /pmc/articles/PMC10057399/ /pubmed/36984980 http://dx.doi.org/10.3390/mi14030573 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Edgar, Robert H.
Samson, Anie-Pier
Kocsis, Tori
Viator, John A.
Photoacoustic Flow Cytometry Using Functionalized Microspheres for Selective Detection of Bacteria
title Photoacoustic Flow Cytometry Using Functionalized Microspheres for Selective Detection of Bacteria
title_full Photoacoustic Flow Cytometry Using Functionalized Microspheres for Selective Detection of Bacteria
title_fullStr Photoacoustic Flow Cytometry Using Functionalized Microspheres for Selective Detection of Bacteria
title_full_unstemmed Photoacoustic Flow Cytometry Using Functionalized Microspheres for Selective Detection of Bacteria
title_short Photoacoustic Flow Cytometry Using Functionalized Microspheres for Selective Detection of Bacteria
title_sort photoacoustic flow cytometry using functionalized microspheres for selective detection of bacteria
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10057399/
https://www.ncbi.nlm.nih.gov/pubmed/36984980
http://dx.doi.org/10.3390/mi14030573
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