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Layer-by-Layer Hollow Mesoporous Silica Nanoparticles with Tunable Degradation Profile
Silica nanoparticles (SNPs) have shown promise in biomedical applications such as drug delivery and imaging due to their versatile synthetic methods, tunable physicochemical properties, and ability to load both hydrophilic and hydrophobic cargo with high efficiency. To improve the utility of these n...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10057406/ https://www.ncbi.nlm.nih.gov/pubmed/36986693 http://dx.doi.org/10.3390/pharmaceutics15030832 |
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author | Grunberger, Jason William Ghandehari, Hamidreza |
author_facet | Grunberger, Jason William Ghandehari, Hamidreza |
author_sort | Grunberger, Jason William |
collection | PubMed |
description | Silica nanoparticles (SNPs) have shown promise in biomedical applications such as drug delivery and imaging due to their versatile synthetic methods, tunable physicochemical properties, and ability to load both hydrophilic and hydrophobic cargo with high efficiency. To improve the utility of these nanostructures, there is a need to control the degradation profile relative to specific microenvironments. The design of such nanostructures for controlled combination drug delivery would benefit from minimizing degradation and cargo release in circulation while increasing intracellular biodegradation. Herein, we fabricated two types of layer-by-layer hollow mesoporous SNPs (HMSNPs) containing two and three layers with variations in disulfide precursor ratios. These disulfide bonds are redox-sensitive, resulting in a controllable degradation profile relative to the number of disulfide bonds present. Particles were characterized for morphology, size and size distribution, atomic composition, pore structure, and surface area. No difference was observed between in vitro cytotoxicity profiles of the fabricated nanoparticles at 24 h in the concentration range below 100 µg mL(−1). The degradation profiles of particles were evaluated in simulated body fluid in the presence of glutathione. The results demonstrate that the composition and number of layers influence degradation rates, and particles containing a higher number of disulfide bridges were more responsive to enzymatic degradation. These results indicate the potential utility of layer-by-layer HMSNPs for delivery applications where tunable degradation is desired. |
format | Online Article Text |
id | pubmed-10057406 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100574062023-03-30 Layer-by-Layer Hollow Mesoporous Silica Nanoparticles with Tunable Degradation Profile Grunberger, Jason William Ghandehari, Hamidreza Pharmaceutics Article Silica nanoparticles (SNPs) have shown promise in biomedical applications such as drug delivery and imaging due to their versatile synthetic methods, tunable physicochemical properties, and ability to load both hydrophilic and hydrophobic cargo with high efficiency. To improve the utility of these nanostructures, there is a need to control the degradation profile relative to specific microenvironments. The design of such nanostructures for controlled combination drug delivery would benefit from minimizing degradation and cargo release in circulation while increasing intracellular biodegradation. Herein, we fabricated two types of layer-by-layer hollow mesoporous SNPs (HMSNPs) containing two and three layers with variations in disulfide precursor ratios. These disulfide bonds are redox-sensitive, resulting in a controllable degradation profile relative to the number of disulfide bonds present. Particles were characterized for morphology, size and size distribution, atomic composition, pore structure, and surface area. No difference was observed between in vitro cytotoxicity profiles of the fabricated nanoparticles at 24 h in the concentration range below 100 µg mL(−1). The degradation profiles of particles were evaluated in simulated body fluid in the presence of glutathione. The results demonstrate that the composition and number of layers influence degradation rates, and particles containing a higher number of disulfide bridges were more responsive to enzymatic degradation. These results indicate the potential utility of layer-by-layer HMSNPs for delivery applications where tunable degradation is desired. MDPI 2023-03-03 /pmc/articles/PMC10057406/ /pubmed/36986693 http://dx.doi.org/10.3390/pharmaceutics15030832 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Grunberger, Jason William Ghandehari, Hamidreza Layer-by-Layer Hollow Mesoporous Silica Nanoparticles with Tunable Degradation Profile |
title | Layer-by-Layer Hollow Mesoporous Silica Nanoparticles with Tunable Degradation Profile |
title_full | Layer-by-Layer Hollow Mesoporous Silica Nanoparticles with Tunable Degradation Profile |
title_fullStr | Layer-by-Layer Hollow Mesoporous Silica Nanoparticles with Tunable Degradation Profile |
title_full_unstemmed | Layer-by-Layer Hollow Mesoporous Silica Nanoparticles with Tunable Degradation Profile |
title_short | Layer-by-Layer Hollow Mesoporous Silica Nanoparticles with Tunable Degradation Profile |
title_sort | layer-by-layer hollow mesoporous silica nanoparticles with tunable degradation profile |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10057406/ https://www.ncbi.nlm.nih.gov/pubmed/36986693 http://dx.doi.org/10.3390/pharmaceutics15030832 |
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