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Layer-by-Layer Hollow Mesoporous Silica Nanoparticles with Tunable Degradation Profile

Silica nanoparticles (SNPs) have shown promise in biomedical applications such as drug delivery and imaging due to their versatile synthetic methods, tunable physicochemical properties, and ability to load both hydrophilic and hydrophobic cargo with high efficiency. To improve the utility of these n...

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Autores principales: Grunberger, Jason William, Ghandehari, Hamidreza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10057406/
https://www.ncbi.nlm.nih.gov/pubmed/36986693
http://dx.doi.org/10.3390/pharmaceutics15030832
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author Grunberger, Jason William
Ghandehari, Hamidreza
author_facet Grunberger, Jason William
Ghandehari, Hamidreza
author_sort Grunberger, Jason William
collection PubMed
description Silica nanoparticles (SNPs) have shown promise in biomedical applications such as drug delivery and imaging due to their versatile synthetic methods, tunable physicochemical properties, and ability to load both hydrophilic and hydrophobic cargo with high efficiency. To improve the utility of these nanostructures, there is a need to control the degradation profile relative to specific microenvironments. The design of such nanostructures for controlled combination drug delivery would benefit from minimizing degradation and cargo release in circulation while increasing intracellular biodegradation. Herein, we fabricated two types of layer-by-layer hollow mesoporous SNPs (HMSNPs) containing two and three layers with variations in disulfide precursor ratios. These disulfide bonds are redox-sensitive, resulting in a controllable degradation profile relative to the number of disulfide bonds present. Particles were characterized for morphology, size and size distribution, atomic composition, pore structure, and surface area. No difference was observed between in vitro cytotoxicity profiles of the fabricated nanoparticles at 24 h in the concentration range below 100 µg mL(−1). The degradation profiles of particles were evaluated in simulated body fluid in the presence of glutathione. The results demonstrate that the composition and number of layers influence degradation rates, and particles containing a higher number of disulfide bridges were more responsive to enzymatic degradation. These results indicate the potential utility of layer-by-layer HMSNPs for delivery applications where tunable degradation is desired.
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spelling pubmed-100574062023-03-30 Layer-by-Layer Hollow Mesoporous Silica Nanoparticles with Tunable Degradation Profile Grunberger, Jason William Ghandehari, Hamidreza Pharmaceutics Article Silica nanoparticles (SNPs) have shown promise in biomedical applications such as drug delivery and imaging due to their versatile synthetic methods, tunable physicochemical properties, and ability to load both hydrophilic and hydrophobic cargo with high efficiency. To improve the utility of these nanostructures, there is a need to control the degradation profile relative to specific microenvironments. The design of such nanostructures for controlled combination drug delivery would benefit from minimizing degradation and cargo release in circulation while increasing intracellular biodegradation. Herein, we fabricated two types of layer-by-layer hollow mesoporous SNPs (HMSNPs) containing two and three layers with variations in disulfide precursor ratios. These disulfide bonds are redox-sensitive, resulting in a controllable degradation profile relative to the number of disulfide bonds present. Particles were characterized for morphology, size and size distribution, atomic composition, pore structure, and surface area. No difference was observed between in vitro cytotoxicity profiles of the fabricated nanoparticles at 24 h in the concentration range below 100 µg mL(−1). The degradation profiles of particles were evaluated in simulated body fluid in the presence of glutathione. The results demonstrate that the composition and number of layers influence degradation rates, and particles containing a higher number of disulfide bridges were more responsive to enzymatic degradation. These results indicate the potential utility of layer-by-layer HMSNPs for delivery applications where tunable degradation is desired. MDPI 2023-03-03 /pmc/articles/PMC10057406/ /pubmed/36986693 http://dx.doi.org/10.3390/pharmaceutics15030832 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Grunberger, Jason William
Ghandehari, Hamidreza
Layer-by-Layer Hollow Mesoporous Silica Nanoparticles with Tunable Degradation Profile
title Layer-by-Layer Hollow Mesoporous Silica Nanoparticles with Tunable Degradation Profile
title_full Layer-by-Layer Hollow Mesoporous Silica Nanoparticles with Tunable Degradation Profile
title_fullStr Layer-by-Layer Hollow Mesoporous Silica Nanoparticles with Tunable Degradation Profile
title_full_unstemmed Layer-by-Layer Hollow Mesoporous Silica Nanoparticles with Tunable Degradation Profile
title_short Layer-by-Layer Hollow Mesoporous Silica Nanoparticles with Tunable Degradation Profile
title_sort layer-by-layer hollow mesoporous silica nanoparticles with tunable degradation profile
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10057406/
https://www.ncbi.nlm.nih.gov/pubmed/36986693
http://dx.doi.org/10.3390/pharmaceutics15030832
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