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A novel lytic polysaccharide monooxygenase from enrichment microbiota and its application for shrimp shell powder biodegradation

Lytic polysaccharide monooxygenases (LPMO) are expected to change the current status of chitin resource utilization. This study reports that targeted enrichment of the microbiota was performed with chitin by the selective gradient culture technique, and a novel LPMO (M2822) was identified from the e...

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Autores principales: Zhang, Yang, Pan, Delong, Xiao, Peiyao, Xu, Qianqian, Geng, Fan, Zhang, Xinyu, Zhou, Xiuling, Xu, Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10057547/
https://www.ncbi.nlm.nih.gov/pubmed/37007517
http://dx.doi.org/10.3389/fmicb.2023.1097492
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author Zhang, Yang
Pan, Delong
Xiao, Peiyao
Xu, Qianqian
Geng, Fan
Zhang, Xinyu
Zhou, Xiuling
Xu, Hong
author_facet Zhang, Yang
Pan, Delong
Xiao, Peiyao
Xu, Qianqian
Geng, Fan
Zhang, Xinyu
Zhou, Xiuling
Xu, Hong
author_sort Zhang, Yang
collection PubMed
description Lytic polysaccharide monooxygenases (LPMO) are expected to change the current status of chitin resource utilization. This study reports that targeted enrichment of the microbiota was performed with chitin by the selective gradient culture technique, and a novel LPMO (M2822) was identified from the enrichment microbiota metagenome. First, soil samples were screened based on soil bacterial species and chitinase biodiversity. Then gradient enrichment culture with different chitin concentrations was carried out. The efficiency of chitin powder degradation was increased by 10.67 times through enrichment, and chitin degradation species Chitiniphilus and Chitinolyticbacter were enriched significantly. A novel LPMO (M2822) was found in the metagenome of the enriched microbiota. Phylogenetic analysis showed that M2822 had a unique phylogenetic position in auxiliary activity (AA) 10 family. The analysis of enzymatic hydrolysate showed that M2822 had chitin activity. When M2822 synergized with commercial chitinase to degrade chitin, the yield of N-acetyl glycosamine was 83.6% higher than chitinase alone. The optimum temperature and pH for M2822 activity were 35°C and 6.0. The synergistic action of M2822 and chitin-degrading enzymes secreted by Chitiniphilus sp. LZ32 could efficiently hydrolyze shrimp shell powder. After 12 h of enzymatic hydrolysis, chitin oligosaccharides (COS) yield reached 4,724 μg/mL. To our knowledge, this work is the first study to mine chitin activity LPMO in the metagenome of enriched microbiota. The obtained M2822 showed application prospects in the efficient production of COS.
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spelling pubmed-100575472023-03-30 A novel lytic polysaccharide monooxygenase from enrichment microbiota and its application for shrimp shell powder biodegradation Zhang, Yang Pan, Delong Xiao, Peiyao Xu, Qianqian Geng, Fan Zhang, Xinyu Zhou, Xiuling Xu, Hong Front Microbiol Microbiology Lytic polysaccharide monooxygenases (LPMO) are expected to change the current status of chitin resource utilization. This study reports that targeted enrichment of the microbiota was performed with chitin by the selective gradient culture technique, and a novel LPMO (M2822) was identified from the enrichment microbiota metagenome. First, soil samples were screened based on soil bacterial species and chitinase biodiversity. Then gradient enrichment culture with different chitin concentrations was carried out. The efficiency of chitin powder degradation was increased by 10.67 times through enrichment, and chitin degradation species Chitiniphilus and Chitinolyticbacter were enriched significantly. A novel LPMO (M2822) was found in the metagenome of the enriched microbiota. Phylogenetic analysis showed that M2822 had a unique phylogenetic position in auxiliary activity (AA) 10 family. The analysis of enzymatic hydrolysate showed that M2822 had chitin activity. When M2822 synergized with commercial chitinase to degrade chitin, the yield of N-acetyl glycosamine was 83.6% higher than chitinase alone. The optimum temperature and pH for M2822 activity were 35°C and 6.0. The synergistic action of M2822 and chitin-degrading enzymes secreted by Chitiniphilus sp. LZ32 could efficiently hydrolyze shrimp shell powder. After 12 h of enzymatic hydrolysis, chitin oligosaccharides (COS) yield reached 4,724 μg/mL. To our knowledge, this work is the first study to mine chitin activity LPMO in the metagenome of enriched microbiota. The obtained M2822 showed application prospects in the efficient production of COS. Frontiers Media S.A. 2023-03-15 /pmc/articles/PMC10057547/ /pubmed/37007517 http://dx.doi.org/10.3389/fmicb.2023.1097492 Text en Copyright © 2023 Zhang, Pan, Xiao, Xu, Geng, Zhang, Zhou and Xu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Zhang, Yang
Pan, Delong
Xiao, Peiyao
Xu, Qianqian
Geng, Fan
Zhang, Xinyu
Zhou, Xiuling
Xu, Hong
A novel lytic polysaccharide monooxygenase from enrichment microbiota and its application for shrimp shell powder biodegradation
title A novel lytic polysaccharide monooxygenase from enrichment microbiota and its application for shrimp shell powder biodegradation
title_full A novel lytic polysaccharide monooxygenase from enrichment microbiota and its application for shrimp shell powder biodegradation
title_fullStr A novel lytic polysaccharide monooxygenase from enrichment microbiota and its application for shrimp shell powder biodegradation
title_full_unstemmed A novel lytic polysaccharide monooxygenase from enrichment microbiota and its application for shrimp shell powder biodegradation
title_short A novel lytic polysaccharide monooxygenase from enrichment microbiota and its application for shrimp shell powder biodegradation
title_sort novel lytic polysaccharide monooxygenase from enrichment microbiota and its application for shrimp shell powder biodegradation
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10057547/
https://www.ncbi.nlm.nih.gov/pubmed/37007517
http://dx.doi.org/10.3389/fmicb.2023.1097492
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