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Transcriptomic Profiling of Fusarium pseudograminearum in Response to Carbendazim, Pyraclostrobin, Tebuconazole, and Phenamacril

Fusarium pseudograminearum has been identified as a significant pathogen. It causes Fusarium crown rot (FCR), which occurs in several major wheat-producing areas in China. Chemical control is the primary measure with which to control this disease. In this study, transcriptome sequencing (RNA-Seq) wa...

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Autores principales: Zhang, Yuan, He, Kai, Guo, Xuhao, Jiang, Jia, Qian, Le, Xu, Jianqiang, Che, Zhiping, Huang, Xiaobo, Liu, Shengming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10057576/
https://www.ncbi.nlm.nih.gov/pubmed/36983502
http://dx.doi.org/10.3390/jof9030334
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author Zhang, Yuan
He, Kai
Guo, Xuhao
Jiang, Jia
Qian, Le
Xu, Jianqiang
Che, Zhiping
Huang, Xiaobo
Liu, Shengming
author_facet Zhang, Yuan
He, Kai
Guo, Xuhao
Jiang, Jia
Qian, Le
Xu, Jianqiang
Che, Zhiping
Huang, Xiaobo
Liu, Shengming
author_sort Zhang, Yuan
collection PubMed
description Fusarium pseudograminearum has been identified as a significant pathogen. It causes Fusarium crown rot (FCR), which occurs in several major wheat-producing areas in China. Chemical control is the primary measure with which to control this disease. In this study, transcriptome sequencing (RNA-Seq) was used to determine the different mechanisms of action of four frequently used fungicides including carbendazim, pyraclostrobin, tebuconazole, and phenamacril on F. pseudograminearum. In brief, 381, 1896, 842, and 814 differentially expressed genes (DEGs) were identified under the carbendazim, pyraclostrobin, tebuconazole, and phenamacril treatments, respectively. After the joint analysis, 67 common DEGs were obtained, and further functional analysis showed that the ABC transported pathway was significantly enriched. Moreover, FPSE_04130 (FER6) and FPSE_11895 (MDR1), two important ABC multidrug transporter genes whose expression levels simultaneously increased, were mined under the different treatments, which unambiguously demonstrated the common effects. In addition, Mfuzz clustering analysis and WGCNA analysis revealed that the core DEGs are involved in several critical pathways in each of the four treatment groups. Taken together, these genes may play a crucial function in the mechanisms of F. pseudograminearum‘s response to the fungicides stress.
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spelling pubmed-100575762023-03-30 Transcriptomic Profiling of Fusarium pseudograminearum in Response to Carbendazim, Pyraclostrobin, Tebuconazole, and Phenamacril Zhang, Yuan He, Kai Guo, Xuhao Jiang, Jia Qian, Le Xu, Jianqiang Che, Zhiping Huang, Xiaobo Liu, Shengming J Fungi (Basel) Article Fusarium pseudograminearum has been identified as a significant pathogen. It causes Fusarium crown rot (FCR), which occurs in several major wheat-producing areas in China. Chemical control is the primary measure with which to control this disease. In this study, transcriptome sequencing (RNA-Seq) was used to determine the different mechanisms of action of four frequently used fungicides including carbendazim, pyraclostrobin, tebuconazole, and phenamacril on F. pseudograminearum. In brief, 381, 1896, 842, and 814 differentially expressed genes (DEGs) were identified under the carbendazim, pyraclostrobin, tebuconazole, and phenamacril treatments, respectively. After the joint analysis, 67 common DEGs were obtained, and further functional analysis showed that the ABC transported pathway was significantly enriched. Moreover, FPSE_04130 (FER6) and FPSE_11895 (MDR1), two important ABC multidrug transporter genes whose expression levels simultaneously increased, were mined under the different treatments, which unambiguously demonstrated the common effects. In addition, Mfuzz clustering analysis and WGCNA analysis revealed that the core DEGs are involved in several critical pathways in each of the four treatment groups. Taken together, these genes may play a crucial function in the mechanisms of F. pseudograminearum‘s response to the fungicides stress. MDPI 2023-03-08 /pmc/articles/PMC10057576/ /pubmed/36983502 http://dx.doi.org/10.3390/jof9030334 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Yuan
He, Kai
Guo, Xuhao
Jiang, Jia
Qian, Le
Xu, Jianqiang
Che, Zhiping
Huang, Xiaobo
Liu, Shengming
Transcriptomic Profiling of Fusarium pseudograminearum in Response to Carbendazim, Pyraclostrobin, Tebuconazole, and Phenamacril
title Transcriptomic Profiling of Fusarium pseudograminearum in Response to Carbendazim, Pyraclostrobin, Tebuconazole, and Phenamacril
title_full Transcriptomic Profiling of Fusarium pseudograminearum in Response to Carbendazim, Pyraclostrobin, Tebuconazole, and Phenamacril
title_fullStr Transcriptomic Profiling of Fusarium pseudograminearum in Response to Carbendazim, Pyraclostrobin, Tebuconazole, and Phenamacril
title_full_unstemmed Transcriptomic Profiling of Fusarium pseudograminearum in Response to Carbendazim, Pyraclostrobin, Tebuconazole, and Phenamacril
title_short Transcriptomic Profiling of Fusarium pseudograminearum in Response to Carbendazim, Pyraclostrobin, Tebuconazole, and Phenamacril
title_sort transcriptomic profiling of fusarium pseudograminearum in response to carbendazim, pyraclostrobin, tebuconazole, and phenamacril
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10057576/
https://www.ncbi.nlm.nih.gov/pubmed/36983502
http://dx.doi.org/10.3390/jof9030334
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