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Axl contributes to efficient migration and invasion of melanoma cells

Axl, a member of the TAM receptor family has been broadly suggested to play a key role in tumor metastasis. However, the function of Axl in the invasion and metastasis of melanoma, the most lethal skin cancer, remains largely unknown. In the present study, we found that melanoma cell lines present v...

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Detalles Bibliográficos
Autores principales: Shao, Hanshuang, Teramae, Diana, Wells, Alan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10057740/
https://www.ncbi.nlm.nih.gov/pubmed/36989239
http://dx.doi.org/10.1371/journal.pone.0283749
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author Shao, Hanshuang
Teramae, Diana
Wells, Alan
author_facet Shao, Hanshuang
Teramae, Diana
Wells, Alan
author_sort Shao, Hanshuang
collection PubMed
description Axl, a member of the TAM receptor family has been broadly suggested to play a key role in tumor metastasis. However, the function of Axl in the invasion and metastasis of melanoma, the most lethal skin cancer, remains largely unknown. In the present study, we found that melanoma cell lines present variable protein levels of Axl and Tyro3; interestingly, MerTK is not noted at detectable levels in any of tested MGP (metastatic growth phase) cell lines. Treatment with recombinant human Gas6 significantly activates Akt in the Axl-expressing WM852 and IgR3 lines but just slightly in WM1158. IgR3, WM852 and WM1158 demonstrate different autocrine signaling. Knockdown of Axl by siRNA or the treatment with Axl-specific inhibitor R428 dramatically inhibits the migration and invasion of both IgR3 and WM852 in vitro. These findings suggest that Axl enhances the invasion of melanoma cells.
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spelling pubmed-100577402023-03-30 Axl contributes to efficient migration and invasion of melanoma cells Shao, Hanshuang Teramae, Diana Wells, Alan PLoS One Research Article Axl, a member of the TAM receptor family has been broadly suggested to play a key role in tumor metastasis. However, the function of Axl in the invasion and metastasis of melanoma, the most lethal skin cancer, remains largely unknown. In the present study, we found that melanoma cell lines present variable protein levels of Axl and Tyro3; interestingly, MerTK is not noted at detectable levels in any of tested MGP (metastatic growth phase) cell lines. Treatment with recombinant human Gas6 significantly activates Akt in the Axl-expressing WM852 and IgR3 lines but just slightly in WM1158. IgR3, WM852 and WM1158 demonstrate different autocrine signaling. Knockdown of Axl by siRNA or the treatment with Axl-specific inhibitor R428 dramatically inhibits the migration and invasion of both IgR3 and WM852 in vitro. These findings suggest that Axl enhances the invasion of melanoma cells. Public Library of Science 2023-03-29 /pmc/articles/PMC10057740/ /pubmed/36989239 http://dx.doi.org/10.1371/journal.pone.0283749 Text en © 2023 Shao et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Shao, Hanshuang
Teramae, Diana
Wells, Alan
Axl contributes to efficient migration and invasion of melanoma cells
title Axl contributes to efficient migration and invasion of melanoma cells
title_full Axl contributes to efficient migration and invasion of melanoma cells
title_fullStr Axl contributes to efficient migration and invasion of melanoma cells
title_full_unstemmed Axl contributes to efficient migration and invasion of melanoma cells
title_short Axl contributes to efficient migration and invasion of melanoma cells
title_sort axl contributes to efficient migration and invasion of melanoma cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10057740/
https://www.ncbi.nlm.nih.gov/pubmed/36989239
http://dx.doi.org/10.1371/journal.pone.0283749
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