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Liver Transcriptome Analysis of the Black Porgy (Acanthopagrus schlegelii) under Acute Low-Temperature Stress

High nutritional value and the development of efficient biotechnological methods of controlled production have made black porgy (Acanthopagrus schlegelii) an economically important fish in Chinese aquaculture in recent years. However, aquaculture production of the species faces multiple issues assoc...

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Autores principales: Wang, Yue, Chen, Ziqiang, Wei, Mingliang, Lin, Zhijie, Shen, Mingjun, Zhu, Fei, Jia, Chaofeng, Meng, Qian, Xu, Dafeng, Du, Shuran, Liu, Yanli, Chen, Shuyin, Zhang, Caojin, Zhang, Zhiyong, Zhang, Zhiwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10057800/
https://www.ncbi.nlm.nih.gov/pubmed/36983876
http://dx.doi.org/10.3390/life13030721
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author Wang, Yue
Chen, Ziqiang
Wei, Mingliang
Lin, Zhijie
Shen, Mingjun
Zhu, Fei
Jia, Chaofeng
Meng, Qian
Xu, Dafeng
Du, Shuran
Liu, Yanli
Chen, Shuyin
Zhang, Caojin
Zhang, Zhiyong
Zhang, Zhiwei
author_facet Wang, Yue
Chen, Ziqiang
Wei, Mingliang
Lin, Zhijie
Shen, Mingjun
Zhu, Fei
Jia, Chaofeng
Meng, Qian
Xu, Dafeng
Du, Shuran
Liu, Yanli
Chen, Shuyin
Zhang, Caojin
Zhang, Zhiyong
Zhang, Zhiwei
author_sort Wang, Yue
collection PubMed
description High nutritional value and the development of efficient biotechnological methods of controlled production have made black porgy (Acanthopagrus schlegelii) an economically important fish in Chinese aquaculture in recent years. However, aquaculture production of the species faces multiple issues associated with reduced growth rate, low reproduction ability, and high mortality during production, which are associated with the species’ limited tolerance to low temperatures. To date, comprehensive information on the genetic-based mechanisms of cold tolerance and adaptation to low temperature in the species are still unavailable. In this study, the HiSeq™2500 (Illumina) sequencing platform was used to analyze the transcriptomic profile of the liver tissue in the black porgy subjected to different extents of cold shock, including a control temperature group (AS, T = 15 °C), an intermediate temperature group (AL1, T = 10 °C), and an acute low-temperature stress group (AL2, T = 5 °C). For this purpose, three standardized cDNA libraries of AS, AL1, and AL2 were established. We obtained 43,258,908, 48,239,072, and 38,983,833 clean reads from the AS group, AL1 group, and AL2 group, respectively. After pairwise comparison, 70 differentially expressed genes (DEGs) were identified in the examined fish groups. Among them, 60 genes were found to be significantly differentially expressed after trend analysis. GO annotation and enrichment results showed that they were mainly enriched into three categories: biological processes (12 subcategories), molecular functions (7 subcategories), and cellular components (7 subcategories). KEGG analysis results indicated that all significantly differentially expressed genes were annotated to 102 signaling pathways, including biological rhythm, cholesterol metabolism, glycerolipid metabolism, animal autophagy, FoxO signaling pathway, steroid biosynthesis, and regulation of adipocyte lipolysis and apoptosis. Four of them, namely: G6PC, GPX1, GCK, and HSPE1 were randomly selected for further qRT-PCR verification of data reliability obtained by RNA-Seq technology. In this study, we found that environmental acute cold stress mainly affected the black porgy’s biological processes related to metabolism, apoptosis, and signal transduction. The data that we have reported provides baseline information for further studies concerning the genetic responses of the black porgy under cold stress conditions, the improvement of its aquaculture production, and other economically important matters regarding their limited tolerance to cold shock.
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spelling pubmed-100578002023-03-30 Liver Transcriptome Analysis of the Black Porgy (Acanthopagrus schlegelii) under Acute Low-Temperature Stress Wang, Yue Chen, Ziqiang Wei, Mingliang Lin, Zhijie Shen, Mingjun Zhu, Fei Jia, Chaofeng Meng, Qian Xu, Dafeng Du, Shuran Liu, Yanli Chen, Shuyin Zhang, Caojin Zhang, Zhiyong Zhang, Zhiwei Life (Basel) Article High nutritional value and the development of efficient biotechnological methods of controlled production have made black porgy (Acanthopagrus schlegelii) an economically important fish in Chinese aquaculture in recent years. However, aquaculture production of the species faces multiple issues associated with reduced growth rate, low reproduction ability, and high mortality during production, which are associated with the species’ limited tolerance to low temperatures. To date, comprehensive information on the genetic-based mechanisms of cold tolerance and adaptation to low temperature in the species are still unavailable. In this study, the HiSeq™2500 (Illumina) sequencing platform was used to analyze the transcriptomic profile of the liver tissue in the black porgy subjected to different extents of cold shock, including a control temperature group (AS, T = 15 °C), an intermediate temperature group (AL1, T = 10 °C), and an acute low-temperature stress group (AL2, T = 5 °C). For this purpose, three standardized cDNA libraries of AS, AL1, and AL2 were established. We obtained 43,258,908, 48,239,072, and 38,983,833 clean reads from the AS group, AL1 group, and AL2 group, respectively. After pairwise comparison, 70 differentially expressed genes (DEGs) were identified in the examined fish groups. Among them, 60 genes were found to be significantly differentially expressed after trend analysis. GO annotation and enrichment results showed that they were mainly enriched into three categories: biological processes (12 subcategories), molecular functions (7 subcategories), and cellular components (7 subcategories). KEGG analysis results indicated that all significantly differentially expressed genes were annotated to 102 signaling pathways, including biological rhythm, cholesterol metabolism, glycerolipid metabolism, animal autophagy, FoxO signaling pathway, steroid biosynthesis, and regulation of adipocyte lipolysis and apoptosis. Four of them, namely: G6PC, GPX1, GCK, and HSPE1 were randomly selected for further qRT-PCR verification of data reliability obtained by RNA-Seq technology. In this study, we found that environmental acute cold stress mainly affected the black porgy’s biological processes related to metabolism, apoptosis, and signal transduction. The data that we have reported provides baseline information for further studies concerning the genetic responses of the black porgy under cold stress conditions, the improvement of its aquaculture production, and other economically important matters regarding their limited tolerance to cold shock. MDPI 2023-03-07 /pmc/articles/PMC10057800/ /pubmed/36983876 http://dx.doi.org/10.3390/life13030721 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Yue
Chen, Ziqiang
Wei, Mingliang
Lin, Zhijie
Shen, Mingjun
Zhu, Fei
Jia, Chaofeng
Meng, Qian
Xu, Dafeng
Du, Shuran
Liu, Yanli
Chen, Shuyin
Zhang, Caojin
Zhang, Zhiyong
Zhang, Zhiwei
Liver Transcriptome Analysis of the Black Porgy (Acanthopagrus schlegelii) under Acute Low-Temperature Stress
title Liver Transcriptome Analysis of the Black Porgy (Acanthopagrus schlegelii) under Acute Low-Temperature Stress
title_full Liver Transcriptome Analysis of the Black Porgy (Acanthopagrus schlegelii) under Acute Low-Temperature Stress
title_fullStr Liver Transcriptome Analysis of the Black Porgy (Acanthopagrus schlegelii) under Acute Low-Temperature Stress
title_full_unstemmed Liver Transcriptome Analysis of the Black Porgy (Acanthopagrus schlegelii) under Acute Low-Temperature Stress
title_short Liver Transcriptome Analysis of the Black Porgy (Acanthopagrus schlegelii) under Acute Low-Temperature Stress
title_sort liver transcriptome analysis of the black porgy (acanthopagrus schlegelii) under acute low-temperature stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10057800/
https://www.ncbi.nlm.nih.gov/pubmed/36983876
http://dx.doi.org/10.3390/life13030721
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