Cargando…

Pyramiding of Multiple Genes to Improve Rice Blast Resistance of Photo-Thermo Sensitive Male Sterile Line, without Yield Penalty in Hybrid Rice Production

Rice blast caused by pathogenic fungus Magnaporthe oryzae is one of the most serious diseases in rice. The pyramiding of effective resistance genes into rice varieties is a potential approach to reduce the damage of blast disease. In this study, combinations of three resistance genes, Pigm, Pi48 and...

Descripción completa

Detalles Bibliográficos
Autores principales: Peng, Pei, Jiang, Haoyu, Luo, Lihua, Ye, Changrong, Xiao, Yinghui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10058063/
https://www.ncbi.nlm.nih.gov/pubmed/36987076
http://dx.doi.org/10.3390/plants12061389
_version_ 1785016526301036544
author Peng, Pei
Jiang, Haoyu
Luo, Lihua
Ye, Changrong
Xiao, Yinghui
author_facet Peng, Pei
Jiang, Haoyu
Luo, Lihua
Ye, Changrong
Xiao, Yinghui
author_sort Peng, Pei
collection PubMed
description Rice blast caused by pathogenic fungus Magnaporthe oryzae is one of the most serious diseases in rice. The pyramiding of effective resistance genes into rice varieties is a potential approach to reduce the damage of blast disease. In this study, combinations of three resistance genes, Pigm, Pi48 and Pi49, were introduced into a thermo-sensitive genic male sterile (PTGMS) line Chuang5S through marker-assisted selection. The results showed that the blast resistance of improved lines increased significantly compared with Chuang5S, and the three gene pyramiding lines (Pigm + Pi48 + Pi49) had higher rice blast resistance level than monogenic line and digenic lines (Pigm +Pi48, Pigm + Pi49). The genetic backgrounds of the improved lines were highly similar (>90%) to the recurrent parent Chuang5S by using the RICE10K SNP chip. In addition, agronomic traits evaluation also showed pyramiding lines with two or three genes similar to Chuang5S. The yields of the hybrids developed from improved PTGMS lines and Chuang5S are not significantly different. The newly developed PTGMS lines can be practically used for the breeding of parental lines and hybrid varieties with broad spectrum blast resistance.
format Online
Article
Text
id pubmed-10058063
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-100580632023-03-30 Pyramiding of Multiple Genes to Improve Rice Blast Resistance of Photo-Thermo Sensitive Male Sterile Line, without Yield Penalty in Hybrid Rice Production Peng, Pei Jiang, Haoyu Luo, Lihua Ye, Changrong Xiao, Yinghui Plants (Basel) Article Rice blast caused by pathogenic fungus Magnaporthe oryzae is one of the most serious diseases in rice. The pyramiding of effective resistance genes into rice varieties is a potential approach to reduce the damage of blast disease. In this study, combinations of three resistance genes, Pigm, Pi48 and Pi49, were introduced into a thermo-sensitive genic male sterile (PTGMS) line Chuang5S through marker-assisted selection. The results showed that the blast resistance of improved lines increased significantly compared with Chuang5S, and the three gene pyramiding lines (Pigm + Pi48 + Pi49) had higher rice blast resistance level than monogenic line and digenic lines (Pigm +Pi48, Pigm + Pi49). The genetic backgrounds of the improved lines were highly similar (>90%) to the recurrent parent Chuang5S by using the RICE10K SNP chip. In addition, agronomic traits evaluation also showed pyramiding lines with two or three genes similar to Chuang5S. The yields of the hybrids developed from improved PTGMS lines and Chuang5S are not significantly different. The newly developed PTGMS lines can be practically used for the breeding of parental lines and hybrid varieties with broad spectrum blast resistance. MDPI 2023-03-21 /pmc/articles/PMC10058063/ /pubmed/36987076 http://dx.doi.org/10.3390/plants12061389 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Peng, Pei
Jiang, Haoyu
Luo, Lihua
Ye, Changrong
Xiao, Yinghui
Pyramiding of Multiple Genes to Improve Rice Blast Resistance of Photo-Thermo Sensitive Male Sterile Line, without Yield Penalty in Hybrid Rice Production
title Pyramiding of Multiple Genes to Improve Rice Blast Resistance of Photo-Thermo Sensitive Male Sterile Line, without Yield Penalty in Hybrid Rice Production
title_full Pyramiding of Multiple Genes to Improve Rice Blast Resistance of Photo-Thermo Sensitive Male Sterile Line, without Yield Penalty in Hybrid Rice Production
title_fullStr Pyramiding of Multiple Genes to Improve Rice Blast Resistance of Photo-Thermo Sensitive Male Sterile Line, without Yield Penalty in Hybrid Rice Production
title_full_unstemmed Pyramiding of Multiple Genes to Improve Rice Blast Resistance of Photo-Thermo Sensitive Male Sterile Line, without Yield Penalty in Hybrid Rice Production
title_short Pyramiding of Multiple Genes to Improve Rice Blast Resistance of Photo-Thermo Sensitive Male Sterile Line, without Yield Penalty in Hybrid Rice Production
title_sort pyramiding of multiple genes to improve rice blast resistance of photo-thermo sensitive male sterile line, without yield penalty in hybrid rice production
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10058063/
https://www.ncbi.nlm.nih.gov/pubmed/36987076
http://dx.doi.org/10.3390/plants12061389
work_keys_str_mv AT pengpei pyramidingofmultiplegenestoimprovericeblastresistanceofphotothermosensitivemalesterilelinewithoutyieldpenaltyinhybridriceproduction
AT jianghaoyu pyramidingofmultiplegenestoimprovericeblastresistanceofphotothermosensitivemalesterilelinewithoutyieldpenaltyinhybridriceproduction
AT luolihua pyramidingofmultiplegenestoimprovericeblastresistanceofphotothermosensitivemalesterilelinewithoutyieldpenaltyinhybridriceproduction
AT yechangrong pyramidingofmultiplegenestoimprovericeblastresistanceofphotothermosensitivemalesterilelinewithoutyieldpenaltyinhybridriceproduction
AT xiaoyinghui pyramidingofmultiplegenestoimprovericeblastresistanceofphotothermosensitivemalesterilelinewithoutyieldpenaltyinhybridriceproduction